Accepted Manuscript Title: Rapid quantitation of porcine epidemic diarrhea virus (PEDV) by Virus Counter Author: Xiangbin Zhang Jianwei Hao Jing Zhen Lijuan Yin Qianniu Li Chunyi Xue Yongchang Cao PII: DOI: Reference:
S0166-0934(15)00236-0 http://dx.doi.org/doi:10.1016/j.jviromet.2015.07.003 VIRMET 12834
To appear in:
Journal of Virological Methods
Received date: Revised date: Accepted date:
12-1-2015 2-7-2015 6-7-2015
Please cite this article as: Zhang, X., Hao, J., Zhen, J., Yin, L., Li, Q., Xue, C., Cao, Y.,Rapid quantitation of porcine epidemic diarrhea virus (PEDV) by Virus Counter, Journal of Virological Methods (2015), http://dx.doi.org/10.1016/j.jviromet.2015.07.003 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
1
Rapid quantitation of porcine epidemic diarrhea virus (PEDV) by Virus
3
Counter
4
Xiangbin Zhang, Jianwei Hao, Jing Zhen, Lijuan Yin, Qianniu Li, Chunyi Xue, Yongchang Cao*
5
State Key Laboratory of Biocontrol, School of Life Science, Sun Yat-sen University, Guangzhou
6
510006, P.R.China
* Corresponding author:
13 Yongchang Cao:
15
Rm 504#, Biobuilding,
16
School of Life Sciences
17
East Campus, Sun Yat-sen University
18
Higher Education Mega Center
19
Guangzhou 510006, P R China
20
Tel: 86 20 39332938
23 24 25
cr
d
te
Ac ce p
22
M
14
21
us
12
The number of text pages of this manuscript: 8 pages. There are 1 table and 3figures in this manuscript.
an
7 8 9 10 11
ip t
2
Fax: 86 20 39332841
Email: [email protected]
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Page 1 of 10
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Abstract: Virus Counter was applied for the quantitation of porcine epidemic diarrhea virus (PEDV);
34
the working range and reproducibility of the quantification were characterized, and the
35
performance of Virus Counter was evaluated using the plaque assay as the gold standard. In this
36
study, the single burst curves of PEDV were first constructed with the Virus Counter or plaque
37
assay respectively, and the results from Virus Counter and plaque assay showed similar high
38
correlation for interval of 12-24 hr (r=0.9581, p
S0166-0934(15)00236-0 http://dx.doi.org/doi:10.1016/j.jviromet.2015.07.003 VIRMET 12834
To appear in:
Journal of Virological Methods
Received date: Revised date: Accepted date:
12-1-2015 2-7-2015 6-7-2015
Please cite this article as: Zhang, X., Hao, J., Zhen, J., Yin, L., Li, Q., Xue, C., Cao, Y.,Rapid quantitation of porcine epidemic diarrhea virus (PEDV) by Virus Counter, Journal of Virological Methods (2015), http://dx.doi.org/10.1016/j.jviromet.2015.07.003 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
1
Rapid quantitation of porcine epidemic diarrhea virus (PEDV) by Virus
3
Counter
4
Xiangbin Zhang, Jianwei Hao, Jing Zhen, Lijuan Yin, Qianniu Li, Chunyi Xue, Yongchang Cao*
5
State Key Laboratory of Biocontrol, School of Life Science, Sun Yat-sen University, Guangzhou
6
510006, P.R.China
* Corresponding author:
13 Yongchang Cao:
15
Rm 504#, Biobuilding,
16
School of Life Sciences
17
East Campus, Sun Yat-sen University
18
Higher Education Mega Center
19
Guangzhou 510006, P R China
20
Tel: 86 20 39332938
23 24 25
cr
d
te
Ac ce p
22
M
14
21
us
12
The number of text pages of this manuscript: 8 pages. There are 1 table and 3figures in this manuscript.
an
7 8 9 10 11
ip t
2
Fax: 86 20 39332841
Email: [email protected]
26 27 28 29 30 31 1
Page 1 of 10
32
Abstract: Virus Counter was applied for the quantitation of porcine epidemic diarrhea virus (PEDV);
34
the working range and reproducibility of the quantification were characterized, and the
35
performance of Virus Counter was evaluated using the plaque assay as the gold standard. In this
36
study, the single burst curves of PEDV were first constructed with the Virus Counter or plaque
37
assay respectively, and the results from Virus Counter and plaque assay showed similar high
38
correlation for interval of 12-24 hr (r=0.9581, p
