Journal of Chromatographic Science Advance Access published December 17, 2014 Journal of Chromatographic Science 2014;1– 6 doi:10.1093/chromsci/bmu167
Article
Rapid Determination of Bile Acids in Bile from Various Mammals by Reversed-Phase Ultra-Fast Liquid Chromatography Gu Leng Ri Si1,2, Peng Yao1 and Luwen Shi1* 1
Pharmacy Administration and Clinical Pharmacy of School of Pharmaceutical Science, Peking University, Xueyuan Road Haidian District, Beijing 100191, China, and 2Inner Mongolia Autonomous Region People’s Hospital, Inner Mongolia Autonomous Region, Hohhot 010010, China *Author to whom correspondence should be addressed. Email: [email protected] Received 25 February 2014; revised 19 November 2014
A valid and efficient reversed-phase ultra-fast liquid chromatography method was developed for the simultaneous determination of 13 bile acids in the bile of three mammal species, including rat, pig and human gallstone patients. Chromatographic separation was performed with a Shim-pack XR-ODS column, and the mobile phase consisted of acetonitrile and potassium phosphate buffer ( pH 2.6) at a flow rate of 0.5 mL min21. The linear detection range of most bile acids ranged from 2 to 600 ng mL21 with a good correlation coefficient (>0.9995). The precision of each bile acid was
Article
Rapid Determination of Bile Acids in Bile from Various Mammals by Reversed-Phase Ultra-Fast Liquid Chromatography Gu Leng Ri Si1,2, Peng Yao1 and Luwen Shi1* 1
Pharmacy Administration and Clinical Pharmacy of School of Pharmaceutical Science, Peking University, Xueyuan Road Haidian District, Beijing 100191, China, and 2Inner Mongolia Autonomous Region People’s Hospital, Inner Mongolia Autonomous Region, Hohhot 010010, China *Author to whom correspondence should be addressed. Email: [email protected] Received 25 February 2014; revised 19 November 2014
A valid and efficient reversed-phase ultra-fast liquid chromatography method was developed for the simultaneous determination of 13 bile acids in the bile of three mammal species, including rat, pig and human gallstone patients. Chromatographic separation was performed with a Shim-pack XR-ODS column, and the mobile phase consisted of acetonitrile and potassium phosphate buffer ( pH 2.6) at a flow rate of 0.5 mL min21. The linear detection range of most bile acids ranged from 2 to 600 ng mL21 with a good correlation coefficient (>0.9995). The precision of each bile acid was
