Parasitol Res (2014) 113:903–909 DOI 10.1007/s00436-013-3721-8
ORIGINAL PAPER
Proteomic analysis of the effect of diclazuril on second-generation merozoites of Eimeria tenella Xiao-jiong Shen & Tao Li & Jian-jun Fu & Ke-yu Zhang & Xiao-yang Wang & Yin-chun Liu & Hua-jing Zhang & Chao Fan & Cheng-zhong Fei & Fei-qun Xue
Received: 8 May 2013 / Accepted: 27 November 2013 / Published online: 18 December 2013 # Springer-Verlag Berlin Heidelberg 2013
Abstract Diclazuril has long been used as an effective benzeneacetonitrile anticoccidial for the control of Eimeria tenella that causes coccidiosis. However, the molecular mechanism underlying the anticoccidial effects of diclazuril remains elusive. In this study, a proteomic analysis of the effect of diclazuril on second-generation merozoites of E. tenella was performed. Using two-dimensional gel electrophoresis and real-time quantitative polymerase chain reaction (RTPCR), 13 target proteins were found to be significantly affected by diclazuril treatment, with 11 of these proteins being identified as annotated proteins from E. tenella or other Apicomplexa parasites. These proteins contribute to various functions, including metabolism, protein synthesis, and host cell invasion. Using RT-PCR, we identified the potential pattern of transcriptional regulation induced by diclazuril, and we suggest some promising targets for the intervention of E. tenella infection.
Xiao-jiong Shen and Tao Li contributed equally to this work. Electronic supplementary material The online version of this article (doi:10.1007/s00436-013-3721-8) contains supplementary material, which is available to authorized users. X.
ORIGINAL PAPER
Proteomic analysis of the effect of diclazuril on second-generation merozoites of Eimeria tenella Xiao-jiong Shen & Tao Li & Jian-jun Fu & Ke-yu Zhang & Xiao-yang Wang & Yin-chun Liu & Hua-jing Zhang & Chao Fan & Cheng-zhong Fei & Fei-qun Xue
Received: 8 May 2013 / Accepted: 27 November 2013 / Published online: 18 December 2013 # Springer-Verlag Berlin Heidelberg 2013
Abstract Diclazuril has long been used as an effective benzeneacetonitrile anticoccidial for the control of Eimeria tenella that causes coccidiosis. However, the molecular mechanism underlying the anticoccidial effects of diclazuril remains elusive. In this study, a proteomic analysis of the effect of diclazuril on second-generation merozoites of E. tenella was performed. Using two-dimensional gel electrophoresis and real-time quantitative polymerase chain reaction (RTPCR), 13 target proteins were found to be significantly affected by diclazuril treatment, with 11 of these proteins being identified as annotated proteins from E. tenella or other Apicomplexa parasites. These proteins contribute to various functions, including metabolism, protein synthesis, and host cell invasion. Using RT-PCR, we identified the potential pattern of transcriptional regulation induced by diclazuril, and we suggest some promising targets for the intervention of E. tenella infection.
Xiao-jiong Shen and Tao Li contributed equally to this work. Electronic supplementary material The online version of this article (doi:10.1007/s00436-013-3721-8) contains supplementary material, which is available to authorized users. X.
