FULL PAPER Bacteriology
Protectivity conferred by immunization with intranasal recombinant outer membrane protein H from Pasteurella multocida serovar A:1 in chickens Arunee THANASARASAKULPONG1), Pichayanut POOLPERM2), Pallop TANKAEW1), Takuo SAWADA3) and Nattawooti STHITMATEE1)* 1)Faculty
of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand of Pharmacy, Payap University, Chiang Mai 50000, Thailand 3)Laboratory of Veterinary Microbiology, Nippon Veterinary and Life Science University, Musashino, Tokyo 180–8602, Japan 2)Faculty
(Received 10 October 2014/Accepted 18 November 2014/Published online in J-STAGE 20 December 2014.) Abstract.
Recombinant outer membrane protein H (rOmpH) is a potential fowl cholera vaccine candidate. The present study was aimed at developing rOmpH formulations for intranasal administration. The rOmpH was purified and formulated with either Escherichia coli enterotoxin B (LTB) or CpG oligodeoxynucleotides (ODN) as an adjuvant. Antibody responses in chickens intranasally immunized with rOmpH in combination with 2 different adjuvants were significantly increased (P
Protectivity conferred by immunization with intranasal recombinant outer membrane protein H from Pasteurella multocida serovar A:1 in chickens Arunee THANASARASAKULPONG1), Pichayanut POOLPERM2), Pallop TANKAEW1), Takuo SAWADA3) and Nattawooti STHITMATEE1)* 1)Faculty
of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand of Pharmacy, Payap University, Chiang Mai 50000, Thailand 3)Laboratory of Veterinary Microbiology, Nippon Veterinary and Life Science University, Musashino, Tokyo 180–8602, Japan 2)Faculty
(Received 10 October 2014/Accepted 18 November 2014/Published online in J-STAGE 20 December 2014.) Abstract.
Recombinant outer membrane protein H (rOmpH) is a potential fowl cholera vaccine candidate. The present study was aimed at developing rOmpH formulations for intranasal administration. The rOmpH was purified and formulated with either Escherichia coli enterotoxin B (LTB) or CpG oligodeoxynucleotides (ODN) as an adjuvant. Antibody responses in chickens intranasally immunized with rOmpH in combination with 2 different adjuvants were significantly increased (P
