Protective Effect of 2-APB on Testicular Ischemia-Reperfusion Injury in Rats Erhan Sari,* Hasan Aksit, Haydar Ali Erken, Arzu Yay, Dilek Aksit, Akin Soner Amasyali, Onur Yildiz, Ahmet Kucukyangoz, Derya Akkus and Kamil Seyrek From the Departments of Urology (ES, AK), Physiology (HAE) and Biochemistry (KS), Faculty of Medicine and Departments of Biochemistry (HA, OY) and Pharmacology (DA), Veterinary Faculty, Balikesir University, Balikesir, Department of Histology and Embryology, Faculty of Medicine, Erciyes University (AY, DA), Kayseri and Department of Urology, Faculty of Medicine, Adnan Menderes University (ASA), Aydin, Turkey
Abbreviations and Acronyms 2-APB ¼ 2-aminoethoxydiphenyl borate DMSO ¼ dimethyl sulfoxide GSH ¼ glutathione I/R ¼ ischemia-reperfusion I/R2APB2 ¼ I/R plus 2 mg/kg 2-APB I/R2APB4 ¼ I/R plus 4 mg/kg 2-APB mCa2þ ¼ mitochondrial Ca2þ MDA ¼ malondialdehyde ROS ¼ reactive oxygen species SOC ¼ store operated Ca2þ SOD ¼ superoxide dismutase TAC ¼ total antioxidant capacity TBA ¼ thiobarbituric acid Accepted for publication September 2, 2014. Study received local ethical committee approval. * Correspondence: Department of Urology, Faculty of Medicine, Balikesir University, Balikesir, Turkey (telephone: þ905056410198; e-mail: [email protected]).
Purpose: We performed biochemical and histopathological evaluations to assess the effects of 2-APB on ischemia-reperfusion induced testicular damage. Materials and Methods: A total of 28 rats were randomly divided into 4 groups, including sham treated, ischemia-reperfusion, ischemia-reperfusion plus 2 mg/kg 2-APB and ischemia-reperfusion plus 4 mg/kg 2-APB. Testicular tissue superoxide dismutase, glutathione, malondialdehyde, total antioxidant capacity and DNA fragmentation levels were determined. Testicular tissue samples were examined by histopathology and TUNEL staining. Results: Mean superoxide dismutase, total antioxidant capacity and glutathione were significantly higher in the sham treated group than in the ischemiaperfusion group (p
Abbreviations and Acronyms 2-APB ¼ 2-aminoethoxydiphenyl borate DMSO ¼ dimethyl sulfoxide GSH ¼ glutathione I/R ¼ ischemia-reperfusion I/R2APB2 ¼ I/R plus 2 mg/kg 2-APB I/R2APB4 ¼ I/R plus 4 mg/kg 2-APB mCa2þ ¼ mitochondrial Ca2þ MDA ¼ malondialdehyde ROS ¼ reactive oxygen species SOC ¼ store operated Ca2þ SOD ¼ superoxide dismutase TAC ¼ total antioxidant capacity TBA ¼ thiobarbituric acid Accepted for publication September 2, 2014. Study received local ethical committee approval. * Correspondence: Department of Urology, Faculty of Medicine, Balikesir University, Balikesir, Turkey (telephone: þ905056410198; e-mail: [email protected]).
Purpose: We performed biochemical and histopathological evaluations to assess the effects of 2-APB on ischemia-reperfusion induced testicular damage. Materials and Methods: A total of 28 rats were randomly divided into 4 groups, including sham treated, ischemia-reperfusion, ischemia-reperfusion plus 2 mg/kg 2-APB and ischemia-reperfusion plus 4 mg/kg 2-APB. Testicular tissue superoxide dismutase, glutathione, malondialdehyde, total antioxidant capacity and DNA fragmentation levels were determined. Testicular tissue samples were examined by histopathology and TUNEL staining. Results: Mean superoxide dismutase, total antioxidant capacity and glutathione were significantly higher in the sham treated group than in the ischemiaperfusion group (p
