Article pubs.acs.org/JAFC
Production of Monoclonal Antibody and Development of a New Immunoassay for Apramycin in Food Fei Xu,†,∥ Wenxiao Jiang,†,∥ Jie Zhou,† Kai Wen,† Zhanhui Wang,‡ Haiyang Jiang,‡ and Shuangyang Ding*,†,‡ †
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 1000193, People’s Republic of China ‡ National Reference Laboratory for Residues of Veterinary Drugs, Beijing 100193, People’s Republic of China S Supporting Information *
ABSTRACT: Apramycin (APR) residue in food of animal origin can cause harmful effects on human health. In this study, a monoclonal antibody (mAb) was successfully produced using APR−BSA as immunogen, which was prepared by using the glutaraldehyde method. mAb 2A2 showed low cross-reactivity (
Production of Monoclonal Antibody and Development of a New Immunoassay for Apramycin in Food Fei Xu,†,∥ Wenxiao Jiang,†,∥ Jie Zhou,† Kai Wen,† Zhanhui Wang,‡ Haiyang Jiang,‡ and Shuangyang Ding*,†,‡ †
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 1000193, People’s Republic of China ‡ National Reference Laboratory for Residues of Veterinary Drugs, Beijing 100193, People’s Republic of China S Supporting Information *
ABSTRACT: Apramycin (APR) residue in food of animal origin can cause harmful effects on human health. In this study, a monoclonal antibody (mAb) was successfully produced using APR−BSA as immunogen, which was prepared by using the glutaraldehyde method. mAb 2A2 showed low cross-reactivity (
