Summaries of papers
21
REFERENCE PARRISH, J.A., FiTZPATRiCK, T.B., TANENBAUM, L . & PATHAK, A . (1974) Photochemotherapy of psoriasis with oral methoxsalen and longwave ultraviolet light. New England Journal of Medicine, 291, 1207.
Effects and side-effects of 8-inethoxypsoralen-blacklight-therapy in more than 1500 patients GERHARD WEBER
Skin Clinic of City Hospital Nuernberg j FRG
"^
Since 1972 about 1500 in- and outpatients have been treated with the combined 8-methoxypsoralen and blacklight therapy (for details of the technique see Weber, 1974) and also with new blacklight lamps. This therapy has proved to be very good in psoriasis, acne, bacterial disseminated eczema, parapsoriasis en plaques, mycosis fungoides I-IH, herpes zoster, and good in herpes simplex and papular urticaria. We have not had sufficient experience with vitiligo, pityriasis lichenoides, disseminated hchen planus, carcinomas. This therapy did not show any effect with atopic dermatitis or with mycotic eczema. The rate of relapse in psoriasis, the side effects and their exogenous as well as endogenous causes will be discussed; parallel examinations of metabolism will be demonstrated. The organization of our radiation section for 120 to 150 patients treated every day and the geographical extension of the blacklight therapy in our country will be explained. REFERENCE WEBER, G . (1974) Combined 8-methoxypsoralen and black light therapy of psoriasis. Technique and results. British Journal of Dermatology, 90, 317.
Increase in prostaglandin E2 and F2^ in inflammatory exudate at 4-48 h after UVB irradiation of human skin A.KOBZA BLACK, M.W.GREAVES, C . N . H E N S B Y , A.PAZZAGLIA AND N.PLUMMER Institute of Dermatology, London
Little is known of the relationship of prostaglandin (PG) activity and other mediators to the time course of ultraviolet (UV) induced inflammation. A suction blister method was used to obtain inflammatory exudate from human skin 4-48 h after irradiation with UVB (3 x MED). Results in normal (control) and irradiated skin of the same subjects were compared. Preliminary pharmacological analysis for PG, histamine and bradykinin activity was carried out by cascade superfusion
22
Summaries of papers
bioassay. PGs were further characterized by solvent extraction and gel partition chromatography. PGF2t, was also measured by radio-immunoassay. At 24 h after irradiation there was a 20-fold increase in total PG activity (bioassayed in terms of PGE2) in UVB irradiated skin compared with control. Gel partition chromatography showed an approximately 20-fold increase in PGE2 and PGF2J, activity compared with control. Further conflrmation of these flndings was obtained by radioimmunoassay which revealed a 3-fold increase in PGF2a. Increase in PG activity was detectable 4 h after irradiation but there was little or no increase in activity at 48 h compared with control. No increase in histamine or bradykinin activity was detected at any time. The correlation of PGE and PGF activity with the time course of the inflammatory response to UVB lends support to the view that prostaglandins play an important role in the mediation and regulation of the inflammatory response to UV.
21
REFERENCE PARRISH, J.A., FiTZPATRiCK, T.B., TANENBAUM, L . & PATHAK, A . (1974) Photochemotherapy of psoriasis with oral methoxsalen and longwave ultraviolet light. New England Journal of Medicine, 291, 1207.
Effects and side-effects of 8-inethoxypsoralen-blacklight-therapy in more than 1500 patients GERHARD WEBER
Skin Clinic of City Hospital Nuernberg j FRG
"^
Since 1972 about 1500 in- and outpatients have been treated with the combined 8-methoxypsoralen and blacklight therapy (for details of the technique see Weber, 1974) and also with new blacklight lamps. This therapy has proved to be very good in psoriasis, acne, bacterial disseminated eczema, parapsoriasis en plaques, mycosis fungoides I-IH, herpes zoster, and good in herpes simplex and papular urticaria. We have not had sufficient experience with vitiligo, pityriasis lichenoides, disseminated hchen planus, carcinomas. This therapy did not show any effect with atopic dermatitis or with mycotic eczema. The rate of relapse in psoriasis, the side effects and their exogenous as well as endogenous causes will be discussed; parallel examinations of metabolism will be demonstrated. The organization of our radiation section for 120 to 150 patients treated every day and the geographical extension of the blacklight therapy in our country will be explained. REFERENCE WEBER, G . (1974) Combined 8-methoxypsoralen and black light therapy of psoriasis. Technique and results. British Journal of Dermatology, 90, 317.
Increase in prostaglandin E2 and F2^ in inflammatory exudate at 4-48 h after UVB irradiation of human skin A.KOBZA BLACK, M.W.GREAVES, C . N . H E N S B Y , A.PAZZAGLIA AND N.PLUMMER Institute of Dermatology, London
Little is known of the relationship of prostaglandin (PG) activity and other mediators to the time course of ultraviolet (UV) induced inflammation. A suction blister method was used to obtain inflammatory exudate from human skin 4-48 h after irradiation with UVB (3 x MED). Results in normal (control) and irradiated skin of the same subjects were compared. Preliminary pharmacological analysis for PG, histamine and bradykinin activity was carried out by cascade superfusion
22
Summaries of papers
bioassay. PGs were further characterized by solvent extraction and gel partition chromatography. PGF2t, was also measured by radio-immunoassay. At 24 h after irradiation there was a 20-fold increase in total PG activity (bioassayed in terms of PGE2) in UVB irradiated skin compared with control. Gel partition chromatography showed an approximately 20-fold increase in PGE2 and PGF2J, activity compared with control. Further conflrmation of these flndings was obtained by radioimmunoassay which revealed a 3-fold increase in PGF2a. Increase in PG activity was detectable 4 h after irradiation but there was little or no increase in activity at 48 h compared with control. No increase in histamine or bradykinin activity was detected at any time. The correlation of PGE and PGF activity with the time course of the inflammatory response to UVB lends support to the view that prostaglandins play an important role in the mediation and regulation of the inflammatory response to UV.
