INFECTION AND IMMUNITY, June 1992, p. 2465-2469
Vol. 60, No. 6
0019-9567/92/062465-05$02.00/0
Preliminary Characterization of Pseudomonas aeruginosa Peptide Chemotactins for Polymorphonuclear Leukocytes PATRICIA A. FONTAN,l* CLAUDIA R. AMURA,' VERONICA E. GARCIA,1 M. CRISTINA CERQUETTI,' AND DANIEL 0. SORDELLI" 2 Departamento de Microbiologia, Parasitologia e Inmunologia, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155 P-12 1121 Buenos Aires, Argentina, 1 and Department of Pediatrics, Georgetown University, Washington, D.C. 200072 Received 10 January 1992/Accepted 3 April 1992
In a previous report, we showed that supernatants of Pseudononas aeruginosa cultures exhibit chemotactic activity for polymorphonuclear leukocytes (PMNL). In this study, P. aeruginosa chemotactins were isolated, purified, and partially characterized. The organisms were cultured in Vogel-Bonner defined medium, and cultures were stopped in late log phase. Chemotactins withstood heating, remained unaltered after acid or alkali treatment in a pH range from 4 to 10, and resisted digestion by trypsin or carboxypeptidase, but chemotactic activity was decreased by 73% after incubation with pronase. Only 2% of the total chemotactic activity of culture supernatants could be extracted with chloroform. Chemotactins with molecular sizes
Vol. 60, No. 6
0019-9567/92/062465-05$02.00/0
Preliminary Characterization of Pseudomonas aeruginosa Peptide Chemotactins for Polymorphonuclear Leukocytes PATRICIA A. FONTAN,l* CLAUDIA R. AMURA,' VERONICA E. GARCIA,1 M. CRISTINA CERQUETTI,' AND DANIEL 0. SORDELLI" 2 Departamento de Microbiologia, Parasitologia e Inmunologia, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155 P-12 1121 Buenos Aires, Argentina, 1 and Department of Pediatrics, Georgetown University, Washington, D.C. 200072 Received 10 January 1992/Accepted 3 April 1992
In a previous report, we showed that supernatants of Pseudononas aeruginosa cultures exhibit chemotactic activity for polymorphonuclear leukocytes (PMNL). In this study, P. aeruginosa chemotactins were isolated, purified, and partially characterized. The organisms were cultured in Vogel-Bonner defined medium, and cultures were stopped in late log phase. Chemotactins withstood heating, remained unaltered after acid or alkali treatment in a pH range from 4 to 10, and resisted digestion by trypsin or carboxypeptidase, but chemotactic activity was decreased by 73% after incubation with pronase. Only 2% of the total chemotactic activity of culture supernatants could be extracted with chloroform. Chemotactins with molecular sizes
