THROMBOSIS RESEARCH 58; 233-242,199O 0049-3848/90 $3.00 + .OO Printed in the USA. Copyright (c) 1990 Pergamon Press pk. All rights reserved.
PLATELET PIEBBRANE GLYCOPROTEIN Ib: GENETIC POLYMORPHISII DETECTED IN THE INTACT I'lOLECULE AND IN PROTEOLYTIC FRAG?IENTS
Il. ileyer Department
(Received
of
Medical
9.11 .1989;
and
I. Schellenberg
Genetics, Iledical Erfurt, G.D.R.
accepted
in revised form 8.2.1990
Academy
of
Erfurt,
by Editor F. Markwardt)
ABSTRACT Genetic polymorphism of platelet membrane glycoprotein (GP) Ib was studied in 128 healthy German blood donors using immunoblotting with monospecific anti-glycocalicin antibodies. Only three GP Ib forms smaller (B,C and D) were detected. In a number of Vietnamese individuals analysed for comparison all four found. The frequency polymorphic GP Ib forms were the German distribution of GP Ib phenotypes in for U.S. that reported population was similar to of Caucasian origin. GP Ib polymorphism nas Americans samples of observed in purified glycocalicin also Using trypsin-cleaved glycocalicin the single donors. polymorphic region could be located in the C-terminal glycocalicin fragment carbohydrate-rich (macroglycopeptide).
INTRODUCTION Glycoprotein
PLATELET PIEBBRANE GLYCOPROTEIN Ib: GENETIC POLYMORPHISII DETECTED IN THE INTACT I'lOLECULE AND IN PROTEOLYTIC FRAG?IENTS
Il. ileyer Department
(Received
of
Medical
9.11 .1989;
and
I. Schellenberg
Genetics, Iledical Erfurt, G.D.R.
accepted
in revised form 8.2.1990
Academy
of
Erfurt,
by Editor F. Markwardt)
ABSTRACT Genetic polymorphism of platelet membrane glycoprotein (GP) Ib was studied in 128 healthy German blood donors using immunoblotting with monospecific anti-glycocalicin antibodies. Only three GP Ib forms smaller (B,C and D) were detected. In a number of Vietnamese individuals analysed for comparison all four found. The frequency polymorphic GP Ib forms were the German distribution of GP Ib phenotypes in for U.S. that reported population was similar to of Caucasian origin. GP Ib polymorphism nas Americans samples of observed in purified glycocalicin also Using trypsin-cleaved glycocalicin the single donors. polymorphic region could be located in the C-terminal glycocalicin fragment carbohydrate-rich (macroglycopeptide).
INTRODUCTION Glycoprotein
