Plasma Adenine and Cellular ATP in Red Cell Concentrates Collected and Stored in Modified CPD at 4 C G.L. MOORE, M. E. LEDFORD, A N D D. E. BROOKS From the Blood Reseurch Division, Department of Surgery, Lettermun Army Institute of Reseurch. Presidio of Sun Francisco, California
take of the remaining plasma adenine over a period of 42 days at 4 C.'' Zuck et ~ 1 . indicate ' ~ that red blood cell concentrates (RCC) stored fof 35 days (Hct = 75) in modified CPD containing adenine have a marginal mean survival (71 per cent) with a much larger standard deviation than similar units stored as whole blood (mean survival = 81 per cent). The ATP concentrations in the RCC had fallen to 45 per cent of the starting value (the 50% level is generally considered minimal for acceptable survival). Thus, the uptake of adenine and its effect on red blood cell ATP needs further evaluation in RCC. In this report, eight units of modified CPD bloodI4 were stored as RCC (mean Hct = 70) with weekly monitoring of plasma adenine and red blood cell ATP. The results are compared to whole blood units of the same anticoagulant.
Eipbt units of blood were drawn into modified CPD contahing 25 per cent higher glucose and 17.3 mg adenhe (0.25 m M In blood). Red blood cell concentrates (RCC)were prepared to a mean hematocrit (Hct) of 70, the celb stored at 4 C, and plasma adenine and red Mood cell rdcawine triphosphate (ATP) were mamured weekly for 42 days. The removal of plasma h the preparation of RCC reduced by 39 per cent the 8vdabk dcnlm. As a result measurable plasma adenine was depleted by 21 days. The loss of ATP in RCC occurs at a significantly faster rate than h whole blood stored under the same conditions. When red blood cells are stored at higher Hct or for periods longer than 35 days, increased anticoagulant adenine levels are recommen&d.
MANYS T . U D I E S ~have ~ ~ - been ~~ done in the last 15 years to document the efficacy of adenine to assist in the maintenance of high levels of red blood cell adenosine triphosphate (ATP) during blood storage. In the last four years, our laboratory has evaluated CPD adenine fortified blood with regard to its in vivo survival,14 variation in glucose and adenine levels? adenine t o x i ~ i t y and , ~ ~ the ~ ~ uptake and egress of adenine by red blood cells.6When adenine is mixed with whole blood (0.25 mM), we observed a quick distribution (
take of the remaining plasma adenine over a period of 42 days at 4 C.'' Zuck et ~ 1 . indicate ' ~ that red blood cell concentrates (RCC) stored fof 35 days (Hct = 75) in modified CPD containing adenine have a marginal mean survival (71 per cent) with a much larger standard deviation than similar units stored as whole blood (mean survival = 81 per cent). The ATP concentrations in the RCC had fallen to 45 per cent of the starting value (the 50% level is generally considered minimal for acceptable survival). Thus, the uptake of adenine and its effect on red blood cell ATP needs further evaluation in RCC. In this report, eight units of modified CPD bloodI4 were stored as RCC (mean Hct = 70) with weekly monitoring of plasma adenine and red blood cell ATP. The results are compared to whole blood units of the same anticoagulant.
Eipbt units of blood were drawn into modified CPD contahing 25 per cent higher glucose and 17.3 mg adenhe (0.25 m M In blood). Red blood cell concentrates (RCC)were prepared to a mean hematocrit (Hct) of 70, the celb stored at 4 C, and plasma adenine and red Mood cell rdcawine triphosphate (ATP) were mamured weekly for 42 days. The removal of plasma h the preparation of RCC reduced by 39 per cent the 8vdabk dcnlm. As a result measurable plasma adenine was depleted by 21 days. The loss of ATP in RCC occurs at a significantly faster rate than h whole blood stored under the same conditions. When red blood cells are stored at higher Hct or for periods longer than 35 days, increased anticoagulant adenine levels are recommen&d.
MANYS T . U D I E S ~have ~ ~ - been ~~ done in the last 15 years to document the efficacy of adenine to assist in the maintenance of high levels of red blood cell adenosine triphosphate (ATP) during blood storage. In the last four years, our laboratory has evaluated CPD adenine fortified blood with regard to its in vivo survival,14 variation in glucose and adenine levels? adenine t o x i ~ i t y and , ~ ~ the ~ ~ uptake and egress of adenine by red blood cells.6When adenine is mixed with whole blood (0.25 mM), we observed a quick distribution (
