Environ Sci Pollut Res DOI 10.1007/s11356-015-4243-y
RESEARCH ARTICLE
Physiological and biochemical response of soil-grown barley (Hordeum vulgare L.) to cerium oxide nanoparticles Cyren M. Rico & Ana C. Barrios & Wenjuan Tan & Rosnah Rubenecia & Sang Chul Lee & Armando Varela-Ramirez & Jose R. Peralta-Videa & Jorge L. Gardea-Torresdey
Received: 12 December 2014 / Accepted: 16 February 2015 # Springer-Verlag Berlin Heidelberg 2015
Abstract A soil microcosm study was performed to examine the impacts of cerium oxide nanoparticles (nCeO2) on the physiology, productivity, and macromolecular composition of barley (Hordeum vulgare L.). The plants were cultivated in soil treated with nCeO2 at 0, 125, 250, and 500 mg kg−1 (control, nCeO2-L, nCeO2-M, and nCeO2-H, respectively). Accumulation of Ce in leaves/grains and its effects on plant stress and nutrient loading were analyzed. The data revealed that nCeO2-H promoted plant development resulting in 331 % Responsible editor: Elena Maestri Electronic supplementary material The online version of this article (doi:10.1007/s11356-015-4243-y) contains supplementary material, which is available to authorized users. C. M. Rico : A. C. Barrios : W. Tan : J. R. Peralta-Videa : J. L. Gardea-Torresdey (*) Department of Chemistry, The University of Texas at El Paso, 500 W. University Avenue, El Paso, TX 79968, USA e-mail: [email protected] J. R. Peralta-Videa e-mail: [email protected] C. M. Rico : J. R. Peralta-Videa : J. L. Gardea-Torresdey University of California Center for Environmental Implications of Nanotechnology (UC CEIN), The University of Texas at El Paso, El Paso, USA R. Rubenecia : S. C. Lee School of Applied Biosciences, Kyungpook National University, Daegu 702-701, Republic of Korea A. Varela-Ramirez Department of Biological Sciences, Border Biomedical Research Center, The University of Texas at El Paso, 500 W. University Avenue, El Paso, TX 79968, USA J. R. Peralta-Videa : J. L. Gardea-Torresdey Environmental Science and Engineering PhD Program, The University of Texas at El Paso, 500 W. University Avenue, El Paso, TX 79968, USA
increase in shoot biomass compared with the control. nCeO2 treatment modified the stress levels in leaves without apparent signs of toxicity. However, plants exposed to nCeO2-H treatment did not form grains. Compared with control, nCeO2-M enhanced grain Ce accumulation by as much as 294 % which was accompanied by remarkable increases in P, K, Ca, Mg, S, Fe, Zn, Cu, and Al. Likewise, nCeO2-M enhanced the methionine, aspartic acid, threonine, tyrosine, arginine, and linolenic acid contents in the grains by up to 617, 31, 58, 141, 378, and 2.47 % respectively, compared with the rest of the treatments. The findings illustrate the beneficial and harmful effects of nanoceria in barley. Keywords Amino acids . Engineered nanomaterials . Enzymes . Fatty acids . Oxidative stress . Radical scavenging
Introduction Management of environmental impacts of engineered nanomaterials (ENMs) requires understanding of their potential risks and benefits in plants. ENMs exert beneficial or harmful effects on food crops; however, the current understanding on ENM-edible plants interaction provides little insight on the long-term impacts of ENMs on crop physiology and food quality. There is a limited number of reports available in full-grown plants to make a conclusive assessment of the long-term risks and benefits of ENMs in plants. Only less than 30 studies from more than 200 reported in the last 2 years covered the full life cycle of studied plants (Gardea-Torresdey et al. 2014). Thus, more studies involving plants complete life cycle are needed to fully assess the sustainable use of ENMs in agricultural productivity.
Environ Sci Pollut Res
Cerium oxide nanoparticles (nCeO2) are widely utilized in catalytic applications, mechanical planarization, and fuel additives (Reed et al. 2014). Modeling studies have predicted that nCeO2 could contaminate agricultural lands through biosolid application (Keller et al. 2013). Since nCeO2 has very high stability in soil, i.e.,
RESEARCH ARTICLE
Physiological and biochemical response of soil-grown barley (Hordeum vulgare L.) to cerium oxide nanoparticles Cyren M. Rico & Ana C. Barrios & Wenjuan Tan & Rosnah Rubenecia & Sang Chul Lee & Armando Varela-Ramirez & Jose R. Peralta-Videa & Jorge L. Gardea-Torresdey
Received: 12 December 2014 / Accepted: 16 February 2015 # Springer-Verlag Berlin Heidelberg 2015
Abstract A soil microcosm study was performed to examine the impacts of cerium oxide nanoparticles (nCeO2) on the physiology, productivity, and macromolecular composition of barley (Hordeum vulgare L.). The plants were cultivated in soil treated with nCeO2 at 0, 125, 250, and 500 mg kg−1 (control, nCeO2-L, nCeO2-M, and nCeO2-H, respectively). Accumulation of Ce in leaves/grains and its effects on plant stress and nutrient loading were analyzed. The data revealed that nCeO2-H promoted plant development resulting in 331 % Responsible editor: Elena Maestri Electronic supplementary material The online version of this article (doi:10.1007/s11356-015-4243-y) contains supplementary material, which is available to authorized users. C. M. Rico : A. C. Barrios : W. Tan : J. R. Peralta-Videa : J. L. Gardea-Torresdey (*) Department of Chemistry, The University of Texas at El Paso, 500 W. University Avenue, El Paso, TX 79968, USA e-mail: [email protected] J. R. Peralta-Videa e-mail: [email protected] C. M. Rico : J. R. Peralta-Videa : J. L. Gardea-Torresdey University of California Center for Environmental Implications of Nanotechnology (UC CEIN), The University of Texas at El Paso, El Paso, USA R. Rubenecia : S. C. Lee School of Applied Biosciences, Kyungpook National University, Daegu 702-701, Republic of Korea A. Varela-Ramirez Department of Biological Sciences, Border Biomedical Research Center, The University of Texas at El Paso, 500 W. University Avenue, El Paso, TX 79968, USA J. R. Peralta-Videa : J. L. Gardea-Torresdey Environmental Science and Engineering PhD Program, The University of Texas at El Paso, 500 W. University Avenue, El Paso, TX 79968, USA
increase in shoot biomass compared with the control. nCeO2 treatment modified the stress levels in leaves without apparent signs of toxicity. However, plants exposed to nCeO2-H treatment did not form grains. Compared with control, nCeO2-M enhanced grain Ce accumulation by as much as 294 % which was accompanied by remarkable increases in P, K, Ca, Mg, S, Fe, Zn, Cu, and Al. Likewise, nCeO2-M enhanced the methionine, aspartic acid, threonine, tyrosine, arginine, and linolenic acid contents in the grains by up to 617, 31, 58, 141, 378, and 2.47 % respectively, compared with the rest of the treatments. The findings illustrate the beneficial and harmful effects of nanoceria in barley. Keywords Amino acids . Engineered nanomaterials . Enzymes . Fatty acids . Oxidative stress . Radical scavenging
Introduction Management of environmental impacts of engineered nanomaterials (ENMs) requires understanding of their potential risks and benefits in plants. ENMs exert beneficial or harmful effects on food crops; however, the current understanding on ENM-edible plants interaction provides little insight on the long-term impacts of ENMs on crop physiology and food quality. There is a limited number of reports available in full-grown plants to make a conclusive assessment of the long-term risks and benefits of ENMs in plants. Only less than 30 studies from more than 200 reported in the last 2 years covered the full life cycle of studied plants (Gardea-Torresdey et al. 2014). Thus, more studies involving plants complete life cycle are needed to fully assess the sustainable use of ENMs in agricultural productivity.
Environ Sci Pollut Res
Cerium oxide nanoparticles (nCeO2) are widely utilized in catalytic applications, mechanical planarization, and fuel additives (Reed et al. 2014). Modeling studies have predicted that nCeO2 could contaminate agricultural lands through biosolid application (Keller et al. 2013). Since nCeO2 has very high stability in soil, i.e.,
