Journal of Visualized Experiments
www.jove.com
Materials List for:
Physical, Chemical and Biological Characterization of Six Biochars Produced for the Remediation of Contaminated Sites 1
1
2
Mackenzie J. Denyes , Michèle A. Parisien , Allison Rutter , Barbara A. Zeeb
1
1
Department of Chemistry and Chemical Engineering, Royal Military College of Canada
2
Analytical Services Unit, School of Environmental Studies, Queen's University
Correspondence to: Mackenzie J. Denyes at [email protected] URL: http://www.jove.com/video/52183 DOI: doi:10.3791/52183
Materials Name
Company
Biochar
Burt's Greenhouses
NaOAc
Fisher Scientific
E124-4
Acetic Acid
Fisher Scientific
A38-212
Sodium Hydroxide
Fisher Scientific
SS284-1
Isopropanol
Fisher Scientific
A416P4
80% IPA: 800 ml IPA with 200 ml DDI water.
NH4Cl
Fisher Scientific
A649500
Dissolving 5.35 g NH4Cl into 1 L DDI water.
Alumminum Drying Pan
Fisher Scientific
08-732-110
Drying Oven
Fisher Scientific
508N0024
Desiccator
Fisher Scientific
08-595A
Balance
Mettler
1113032410
Saturating Solution
Fisher Scientific
06-664-25
Vortex
Barnstead/Thermolyne
871000536389
Centrifuge
International Equipment Company
24372808
Rinsing Solution
Fisher Scientific (Ricca Chemistry Company)
06-664-24
Conductivity Meter
WESCAN
88298
Replacing Solution
Fisher Scientific
06-664-24
ICP-AES
Varian
EL00053841
ASAP 2000 Surface Area Analyser
Cavlon
885
Degassing at 120 °C for a minimum of 2 hr.
Muffle Furnace
Fisher Scientific
806N0024
Heat for 16 hr covering at 420 °C.
pH Meter
Fisher Scientific
1230185263
Sieve
Fisher Scientific
2288926
4.7 mm sieve being at the top.
Sieve Skaker
Meinzer II
0414-02
Shake for 10 min.
Sodium Sulphate
VWR
EM-SX0761-5
Ottawa Sand
Fisher Scientific
S23-3
Soxhlet Apparatus
Fisher Scientific (Pyrex)
09-557A
DCBP
Suprlco Analytical
48318
Dichloromethane
Sigma Aldrich
40042-40855-U
Copyright © 2014 Journal of Visualized Experiments
Catalog Number
Comments All six biochars were produced at Burt's Greenhouses via BlueFlame Boiler system Dissolving 136.08 g of . NaOAC 3H2O in 750 ml distilled, deionized water (DDI water)
200 °C for 2 hr.
3,000 x g for 5 min.
4 hr at 4–6 cycles/hr.
Page 1 of 3
Journal of Visualized Experiments
www.jove.com
6890 Plus Gas Chromatograph Micro 63 Ni ECD
Agilent
US00034778
Helium
AlphaGaz
SPG-NIT1AL50SMART
Nitrogen
AlphaGaz
SPG-HEL1AL50SMART
Mortor and Pestle
Fisher Scientific (CoorsTeh)
12-948G
Nitric Acid
Fisher Scientific
351288212
No. 40 Filter Paper
Fisher Scientific (Whatman)
09-845A
Quartz/Nickel weigh boats
Fisher Scientific
11-474-210
DMA-80
ATS Scientific
5090264
98–99% Formic Acid
Sigma Aldrich
33015-1L
Sonicator
Fisher Sientific
15338284
Rotating Shaker
New Brunswick Scientific (Innova 2100)
14-278-108
No. 42 Filter Paper
Fisher Scientific (Whatman)
09-855A
WhirlPacks
Fisher Scientific
R55048
Potassium Dihydrogen Orthophospahte
Fisher Scientific
181525
2 M KCl
Fisher Scientific
P282100
Plastic Vials
Fisher Scientific
03-337-20
Ammonium Chloride
Fisher Scientific
PX05115
Allow to warm up to room temperature
Colour Reagent
Fisher Scientific
361028260
Allow to warm up to room temperature
Colorimeter
Fisher Scientific
13-642-400
Turn on to let the lamp warm up and run for 5 min.
ASEAL Auto Analyzer 2
SEAL
4723A12068
Liquified Phenol
Fisher Scientific
MPX05115
NaOH
Fisher Scientific
S318-3
Commercial Bleach
Retail Store
NaOH Pellets
Fisher Scientific
S320-1
Disodium EDTA
Sigma Aldrich
E5124
Sodium Hyprchlorite
Fisher Scientific
SS290-1
Triton (10%)
Fisher Scientific
BP151-100
Sodium Nitroprusside
Fisher Scientific
S350-100
Ammonium Salts
Fisher Scientific
A637-10
Phenoxide
Fisher Scientific
AC388611000
Eisenia Fetida
The Worm Factory
Spade
Retail Store
Bucket
Retail Store
Potting Soil
Retail Store
Copyright © 2014 Journal of Visualized Experiments
1 L volumetric filled to 750 ml with DDI water add 20 ml formic acid and fill to volume with DDI water. 1 hr at 200 rpm.
Alkaline Phenol: Measure 87 ml of liquefied phenol into 1-L volumetric filled 2/3 with DDI water. Add 34 g NaOH, make up to volume with DDI water. Hypochlorite Solution: Using 100ml graduated cylinder measure 31.5 ml of commercial bleach and fill to 100 ml with DDI water.
Page 2 of 3
Journal of Visualized Experiments
www.jove.com
Avoidance Wheel
Environment Canada
Alumminum Foil
Fisher Scientific
01-213-100
Petri Dishes
Fisher Scientific
08-757-11
Pumpkin Seeds
Ontario Seed Company (OSC)
2055
Alfalpha Seeds
Ontario Seed Company (OSC)
6675
Centrifuge Tubes (30 ml)
Fisher Scientific
22-038-906
Beakers (50 ml)
Fisher Scientific (Pyrex)
02-540G
Beakers (30 ml)
Fisher Scientific (Pyrex)
20-540C
Erlenmeyer Flasks (125 ml)
Fisher Scientific (Pyrex)
S76106C
Volumetric Flask (100 ml)
Fisher Scientific (Pyrex)
10-211C
Estuarine Sediment
National Insititute of Standards
1546A
Bleach
Clorox Ultra (5–10% sodium hypochlorite)
Copyright © 2014 Journal of Visualized Experiments
Constructed by a modified design from Environment Canada’s Acute Avoidance Test. 8.5 cm in diameter.
Oven dry at 105 °C.
Standard Reference Material
Page 3 of 3
www.jove.com
Materials List for:
Physical, Chemical and Biological Characterization of Six Biochars Produced for the Remediation of Contaminated Sites 1
1
2
Mackenzie J. Denyes , Michèle A. Parisien , Allison Rutter , Barbara A. Zeeb
1
1
Department of Chemistry and Chemical Engineering, Royal Military College of Canada
2
Analytical Services Unit, School of Environmental Studies, Queen's University
Correspondence to: Mackenzie J. Denyes at [email protected] URL: http://www.jove.com/video/52183 DOI: doi:10.3791/52183
Materials Name
Company
Biochar
Burt's Greenhouses
NaOAc
Fisher Scientific
E124-4
Acetic Acid
Fisher Scientific
A38-212
Sodium Hydroxide
Fisher Scientific
SS284-1
Isopropanol
Fisher Scientific
A416P4
80% IPA: 800 ml IPA with 200 ml DDI water.
NH4Cl
Fisher Scientific
A649500
Dissolving 5.35 g NH4Cl into 1 L DDI water.
Alumminum Drying Pan
Fisher Scientific
08-732-110
Drying Oven
Fisher Scientific
508N0024
Desiccator
Fisher Scientific
08-595A
Balance
Mettler
1113032410
Saturating Solution
Fisher Scientific
06-664-25
Vortex
Barnstead/Thermolyne
871000536389
Centrifuge
International Equipment Company
24372808
Rinsing Solution
Fisher Scientific (Ricca Chemistry Company)
06-664-24
Conductivity Meter
WESCAN
88298
Replacing Solution
Fisher Scientific
06-664-24
ICP-AES
Varian
EL00053841
ASAP 2000 Surface Area Analyser
Cavlon
885
Degassing at 120 °C for a minimum of 2 hr.
Muffle Furnace
Fisher Scientific
806N0024
Heat for 16 hr covering at 420 °C.
pH Meter
Fisher Scientific
1230185263
Sieve
Fisher Scientific
2288926
4.7 mm sieve being at the top.
Sieve Skaker
Meinzer II
0414-02
Shake for 10 min.
Sodium Sulphate
VWR
EM-SX0761-5
Ottawa Sand
Fisher Scientific
S23-3
Soxhlet Apparatus
Fisher Scientific (Pyrex)
09-557A
DCBP
Suprlco Analytical
48318
Dichloromethane
Sigma Aldrich
40042-40855-U
Copyright © 2014 Journal of Visualized Experiments
Catalog Number
Comments All six biochars were produced at Burt's Greenhouses via BlueFlame Boiler system Dissolving 136.08 g of . NaOAC 3H2O in 750 ml distilled, deionized water (DDI water)
200 °C for 2 hr.
3,000 x g for 5 min.
4 hr at 4–6 cycles/hr.
Page 1 of 3
Journal of Visualized Experiments
www.jove.com
6890 Plus Gas Chromatograph Micro 63 Ni ECD
Agilent
US00034778
Helium
AlphaGaz
SPG-NIT1AL50SMART
Nitrogen
AlphaGaz
SPG-HEL1AL50SMART
Mortor and Pestle
Fisher Scientific (CoorsTeh)
12-948G
Nitric Acid
Fisher Scientific
351288212
No. 40 Filter Paper
Fisher Scientific (Whatman)
09-845A
Quartz/Nickel weigh boats
Fisher Scientific
11-474-210
DMA-80
ATS Scientific
5090264
98–99% Formic Acid
Sigma Aldrich
33015-1L
Sonicator
Fisher Sientific
15338284
Rotating Shaker
New Brunswick Scientific (Innova 2100)
14-278-108
No. 42 Filter Paper
Fisher Scientific (Whatman)
09-855A
WhirlPacks
Fisher Scientific
R55048
Potassium Dihydrogen Orthophospahte
Fisher Scientific
181525
2 M KCl
Fisher Scientific
P282100
Plastic Vials
Fisher Scientific
03-337-20
Ammonium Chloride
Fisher Scientific
PX05115
Allow to warm up to room temperature
Colour Reagent
Fisher Scientific
361028260
Allow to warm up to room temperature
Colorimeter
Fisher Scientific
13-642-400
Turn on to let the lamp warm up and run for 5 min.
ASEAL Auto Analyzer 2
SEAL
4723A12068
Liquified Phenol
Fisher Scientific
MPX05115
NaOH
Fisher Scientific
S318-3
Commercial Bleach
Retail Store
NaOH Pellets
Fisher Scientific
S320-1
Disodium EDTA
Sigma Aldrich
E5124
Sodium Hyprchlorite
Fisher Scientific
SS290-1
Triton (10%)
Fisher Scientific
BP151-100
Sodium Nitroprusside
Fisher Scientific
S350-100
Ammonium Salts
Fisher Scientific
A637-10
Phenoxide
Fisher Scientific
AC388611000
Eisenia Fetida
The Worm Factory
Spade
Retail Store
Bucket
Retail Store
Potting Soil
Retail Store
Copyright © 2014 Journal of Visualized Experiments
1 L volumetric filled to 750 ml with DDI water add 20 ml formic acid and fill to volume with DDI water. 1 hr at 200 rpm.
Alkaline Phenol: Measure 87 ml of liquefied phenol into 1-L volumetric filled 2/3 with DDI water. Add 34 g NaOH, make up to volume with DDI water. Hypochlorite Solution: Using 100ml graduated cylinder measure 31.5 ml of commercial bleach and fill to 100 ml with DDI water.
Page 2 of 3
Journal of Visualized Experiments
www.jove.com
Avoidance Wheel
Environment Canada
Alumminum Foil
Fisher Scientific
01-213-100
Petri Dishes
Fisher Scientific
08-757-11
Pumpkin Seeds
Ontario Seed Company (OSC)
2055
Alfalpha Seeds
Ontario Seed Company (OSC)
6675
Centrifuge Tubes (30 ml)
Fisher Scientific
22-038-906
Beakers (50 ml)
Fisher Scientific (Pyrex)
02-540G
Beakers (30 ml)
Fisher Scientific (Pyrex)
20-540C
Erlenmeyer Flasks (125 ml)
Fisher Scientific (Pyrex)
S76106C
Volumetric Flask (100 ml)
Fisher Scientific (Pyrex)
10-211C
Estuarine Sediment
National Insititute of Standards
1546A
Bleach
Clorox Ultra (5–10% sodium hypochlorite)
Copyright © 2014 Journal of Visualized Experiments
Constructed by a modified design from Environment Canada’s Acute Avoidance Test. 8.5 cm in diameter.
Oven dry at 105 °C.
Standard Reference Material
Page 3 of 3
