Terrell‑Hall et al. Fluids Barriers CNS (2017) 14:3 DOI 10.1186/s12987-017-0050-9
Fluids and Barriers of the CNS Open Access
RESEARCH
Permeability across a novel microfluidic blood‑tumor barrier model Tori B. Terrell‑Hall1, Amanda G. Ammer2, Jessica I. G. Griffith1 and Paul R. Lockman1*
Abstract Background: The lack of translatable in vitro blood-tumor barrier (BTB) models creates challenges in the develop‑ ment of drugs to treat tumors of the CNS and our understanding of how the vascular changes at the BBB in the pres‑ ence of a tumor. Methods: In this study, we characterize a novel microfluidic model of the BTB (and BBB model as a reference) that incorporates flow and induces shear stress on endothelial cells. Cell lines utilized include human umbilical vein endothelial cells co-cultured with CTX-TNA2 rat astrocytes (BBB) or Met-1 metastatic murine breast cancer cells (BTB). Cells were capable of communicating across microfluidic compartments via a porous interface. We characterized the device by comparing permeability of three passive permeability markers and one marker subject to efflux. Results: The permeability of Sulforhodamine 101 was significantly (p
Fluids and Barriers of the CNS Open Access
RESEARCH
Permeability across a novel microfluidic blood‑tumor barrier model Tori B. Terrell‑Hall1, Amanda G. Ammer2, Jessica I. G. Griffith1 and Paul R. Lockman1*
Abstract Background: The lack of translatable in vitro blood-tumor barrier (BTB) models creates challenges in the develop‑ ment of drugs to treat tumors of the CNS and our understanding of how the vascular changes at the BBB in the pres‑ ence of a tumor. Methods: In this study, we characterize a novel microfluidic model of the BTB (and BBB model as a reference) that incorporates flow and induces shear stress on endothelial cells. Cell lines utilized include human umbilical vein endothelial cells co-cultured with CTX-TNA2 rat astrocytes (BBB) or Met-1 metastatic murine breast cancer cells (BTB). Cells were capable of communicating across microfluidic compartments via a porous interface. We characterized the device by comparing permeability of three passive permeability markers and one marker subject to efflux. Results: The permeability of Sulforhodamine 101 was significantly (p
