0 1990 Wiley-Liss, Inc.
Cytometry 11:292-299 (1990)
Partition of Protein and DNA During Cytokinesis in Human Breast Cancer Cell Lines Roland Sennerstam and Gert Auer Department of Tumour Pathology, Karolinska Institute and Hospital, S-104 01 Stockholm Sweden Received for publication October 3, 1988; accepted August 16, 1989
Three human breast cancer cell lines (HTB-126, MDA-231, and HTB-122) with DNA index (DI) values between 1.26 and 1.72 were analysed together with a diploid mouse embryonal carcinoma cell line (PCC3) by a TV-video time-lapse technique (pedigree analysis). Cytochemical parameters (DNA and proteins) were studied in individual cells in a rapid scanning microspectrophotometer. Postmitotic sister cell pairs were analysed after Feulgen-naphthol-yellow staining. The DI values of the cell lines were selected to reflect various well-known clinical ploidy entities differing in malignancy potentials. A mitotic disturbance
of the partition of DNA and protein to daughter cells was found in particular in MDA-231 closest to the triploid DNA modal value (DI = 1.37). Duration of mitosis was considerably longer in the near triploid line compared to the other lines. The MDA-231 line was also least sensitive to suboptimal growth conditions. This report calls attention to a possible causality between mitotic error and intraclonal genotype and cell mass heterogeneity. Key terms: Mitotic error, tumor heterogeneity, aneuploidy, euploidy growth rate heterogeneity -
Numerous reports have described how tumor cell populations frequently exhibit heterogeneity, e.g., with respect to growth rate, structural and numerical karyotype abnormalities, or antigeneity (17,18,23), and to drug resistance (12,271. Regarding chromosomal number and DNA, analysed as cytochemical parameters after staining with DNA specific dyes, many reports over the last decade have revealed that highly malignant adult tumors, wherever they arise in the human body, are closely correlated to tumor cell aneuploidy. This has been confirmed in systematic studies, e.g., of human breast cancers (1,2,3,9), lung cancer (30), cancer in thyroid tissues (71, prostatic cancer (311, and urogenital and gynecologic cancers (8,20,22,28). Tumors consisting of cells having aneuploid DNA values were found to behave more aggressively than their near-diploid or polyploid counterDarts. All three human breast cancer cell stem-lines analysed in this paper presented are by definition in the anemloid area between 2C and 4C. However. in a recent report (lo), 409 breast cancer patients were subdivided according to DI into three classes: 1)DIC1.30, 2) 1.31
Cytometry 11:292-299 (1990)
Partition of Protein and DNA During Cytokinesis in Human Breast Cancer Cell Lines Roland Sennerstam and Gert Auer Department of Tumour Pathology, Karolinska Institute and Hospital, S-104 01 Stockholm Sweden Received for publication October 3, 1988; accepted August 16, 1989
Three human breast cancer cell lines (HTB-126, MDA-231, and HTB-122) with DNA index (DI) values between 1.26 and 1.72 were analysed together with a diploid mouse embryonal carcinoma cell line (PCC3) by a TV-video time-lapse technique (pedigree analysis). Cytochemical parameters (DNA and proteins) were studied in individual cells in a rapid scanning microspectrophotometer. Postmitotic sister cell pairs were analysed after Feulgen-naphthol-yellow staining. The DI values of the cell lines were selected to reflect various well-known clinical ploidy entities differing in malignancy potentials. A mitotic disturbance
of the partition of DNA and protein to daughter cells was found in particular in MDA-231 closest to the triploid DNA modal value (DI = 1.37). Duration of mitosis was considerably longer in the near triploid line compared to the other lines. The MDA-231 line was also least sensitive to suboptimal growth conditions. This report calls attention to a possible causality between mitotic error and intraclonal genotype and cell mass heterogeneity. Key terms: Mitotic error, tumor heterogeneity, aneuploidy, euploidy growth rate heterogeneity -
Numerous reports have described how tumor cell populations frequently exhibit heterogeneity, e.g., with respect to growth rate, structural and numerical karyotype abnormalities, or antigeneity (17,18,23), and to drug resistance (12,271. Regarding chromosomal number and DNA, analysed as cytochemical parameters after staining with DNA specific dyes, many reports over the last decade have revealed that highly malignant adult tumors, wherever they arise in the human body, are closely correlated to tumor cell aneuploidy. This has been confirmed in systematic studies, e.g., of human breast cancers (1,2,3,9), lung cancer (30), cancer in thyroid tissues (71, prostatic cancer (311, and urogenital and gynecologic cancers (8,20,22,28). Tumors consisting of cells having aneuploid DNA values were found to behave more aggressively than their near-diploid or polyploid counterDarts. All three human breast cancer cell stem-lines analysed in this paper presented are by definition in the anemloid area between 2C and 4C. However. in a recent report (lo), 409 breast cancer patients were subdivided according to DI into three classes: 1)DIC1.30, 2) 1.31
