Vo1.173, No. 3,1990
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 0-
December 31, 1990
Pages 1157-1160
PANCREASTATIN: CHARACTERIZATION OF BIOLOGICAL ACTIVITY 1
2
3
Tao Zhang , Tohru Mochizuki , Masafumi Kogire , 2 Jin Ishizuka, Noboru Yanaihara , James C. Thompson and
George H. Greeley, Jr.
Department of Surgery The University of Texas Medical Branch Galveston, Texas 77550 Received October 26, 1990
SUMMARY: Pancreastatin (PST) (1-49) was first isolated from the porcine pancreas and can inhibit glucose-induced insulin release. PST (33-49), a PST C-terminal fragment, can also inhibit insulin release. The purpose of this study was to determine the shortest C-terminal biologically active fragment of PST, in terms of inhibition of insulin release from the isolated perfused rat pancreas. Porcine PST (1-49) and C-terminal fragments, PST (33-49), PST (35-49), PST (37-49) and PST (39-49) were synthesized by solid-phase methodology. PST (1-49), PST (33-49) and PST (35-49), at I0 nM, significantly (p
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 0-
December 31, 1990
Pages 1157-1160
PANCREASTATIN: CHARACTERIZATION OF BIOLOGICAL ACTIVITY 1
2
3
Tao Zhang , Tohru Mochizuki , Masafumi Kogire , 2 Jin Ishizuka, Noboru Yanaihara , James C. Thompson and
George H. Greeley, Jr.
Department of Surgery The University of Texas Medical Branch Galveston, Texas 77550 Received October 26, 1990
SUMMARY: Pancreastatin (PST) (1-49) was first isolated from the porcine pancreas and can inhibit glucose-induced insulin release. PST (33-49), a PST C-terminal fragment, can also inhibit insulin release. The purpose of this study was to determine the shortest C-terminal biologically active fragment of PST, in terms of inhibition of insulin release from the isolated perfused rat pancreas. Porcine PST (1-49) and C-terminal fragments, PST (33-49), PST (35-49), PST (37-49) and PST (39-49) were synthesized by solid-phase methodology. PST (1-49), PST (33-49) and PST (35-49), at I0 nM, significantly (p
