Annals of the Rheumatic Diseases 1990; 49: 661-664
661
SCIENTIFIC PAPERS
Oxidative response of polymorphonuclear leucocytes to synovial fluids from patients with rheumatoid arthritis B Dularay, J S Badesha, P A Dieppe, C J Elson
classical or definite RA were studied. They were Abstract Only a minority (7/35, 20%) of synovial fluids receiving a variety of non-steroidal antifrom patients with rheumatoid arthritis (RA) inflammatory and slow acting antirheumatic and none from patients with other arthritides drugs. Synovial fluids were obtained by therastimulated the oxidative response of poly- peutic aspiration of inflamed joints which were morphonuclear leucocytes (PMNs). Super- free from bacterial infection. Samples were oxide anion generation was measured by collected in 0-1 volume of acid citrate dextrose superoxide dismutase inhibitable reduction of and centrifuged to remove the cells. The synocytochrome c. The same synovial fluids vial fluids were divided into aliquots and stored stimulated superoxide release by PMNs re- at -700C. Synovial fluids were also obtained from four gardless of their source, though they elicited a greater response from RA synovial fluid patients with osteoarthritis, four with psoriatic PMNs than from either RA blood PMNs or arthritis, and four with pyrophosphate arthroblood PMNs from normal subjects. The pathy. remaining synovial fluids failed to stimulate any of the PMNs, though some (2/10) stimulated PMNs pretreated with cytochalasin B. PMN PREPARATION The stimulatory activity was removed from Peripheral blood from normal subjects or RA synovial fluids by protein A-Sepharose patients with RA was collected in 3-8% sodium and eluted with the void volume on gel citrate. The PMNs were isolated by sedimenchromatography. It is considered that tation on dextran and density gradient separimmunoglobulin aggregates in some RA ation in Percoll as described by Dooley and synovial fluids may stimulate the oxidative colleagues.'3 This yielded a preparation of 98% response of PMNs in the joint space but that PMNs as judged by Wright's stain with
661
SCIENTIFIC PAPERS
Oxidative response of polymorphonuclear leucocytes to synovial fluids from patients with rheumatoid arthritis B Dularay, J S Badesha, P A Dieppe, C J Elson
classical or definite RA were studied. They were Abstract Only a minority (7/35, 20%) of synovial fluids receiving a variety of non-steroidal antifrom patients with rheumatoid arthritis (RA) inflammatory and slow acting antirheumatic and none from patients with other arthritides drugs. Synovial fluids were obtained by therastimulated the oxidative response of poly- peutic aspiration of inflamed joints which were morphonuclear leucocytes (PMNs). Super- free from bacterial infection. Samples were oxide anion generation was measured by collected in 0-1 volume of acid citrate dextrose superoxide dismutase inhibitable reduction of and centrifuged to remove the cells. The synocytochrome c. The same synovial fluids vial fluids were divided into aliquots and stored stimulated superoxide release by PMNs re- at -700C. Synovial fluids were also obtained from four gardless of their source, though they elicited a greater response from RA synovial fluid patients with osteoarthritis, four with psoriatic PMNs than from either RA blood PMNs or arthritis, and four with pyrophosphate arthroblood PMNs from normal subjects. The pathy. remaining synovial fluids failed to stimulate any of the PMNs, though some (2/10) stimulated PMNs pretreated with cytochalasin B. PMN PREPARATION The stimulatory activity was removed from Peripheral blood from normal subjects or RA synovial fluids by protein A-Sepharose patients with RA was collected in 3-8% sodium and eluted with the void volume on gel citrate. The PMNs were isolated by sedimenchromatography. It is considered that tation on dextran and density gradient separimmunoglobulin aggregates in some RA ation in Percoll as described by Dooley and synovial fluids may stimulate the oxidative colleagues.'3 This yielded a preparation of 98% response of PMNs in the joint space but that PMNs as judged by Wright's stain with
