RESEARCH ARTICLE
Oral Microbiota and Risk for Esophageal Squamous Cell Carcinoma in a High-Risk Area of China Xingdong Chen1,2,3, Björn Winckler1, Ming Lu3,4*, Hongwei Cheng5, Ziyu Yuan2,3, Yajun Yang2,3, Li Jin2,3, Weimin Ye1,3
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1 Department of Medical Epidemiology and Biostatistics, Karolinska Institutet, Stockholm, Sweden, 2 Ministry of Education Key Laboratory of Contemporary Anthropology and State Key Laboratory of Genetic Engineering, Collaborative Innovation Center for Genetics and Development, School of Life Sciences, Fudan University, Shanghai, China, 3 Fudan-Taizhou Institute of Health Sciences, Taizhou, China, 4 Clinical Epidemiology Unit, Qilu Hospital of Shandong University, Jinan, China, 5 Taixing People’s Hospital, Taixing, China * [email protected]
Abstract OPEN ACCESS Citation: Chen X, Winckler B, Lu M, Cheng H, Yuan Z, Yang Y, et al. (2015) Oral Microbiota and Risk for Esophageal Squamous Cell Carcinoma in a HighRisk Area of China. PLoS ONE 10(12): e0143603. doi:10.1371/journal.pone.0143603 Editor: Qing-Yi Wei, Duke Cancer Institute, UNITED STATES Received: September 4, 2015 Accepted: November 6, 2015 Published: December 7, 2015 Copyright: © 2015 Chen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability Statement: All relevant data are within the paper and its Supporting Information files. Funding: This work was supported by a grant from Natural Science Foundation of China (81072358, 81273151), and a grant from Science and Technology Committee of Shandong Province (2012GSF11857). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Poor oral health has been linked with an increased risk of esophageal squamous cell carcinoma (ESCC). We investigated whether alteration of oral microbiota is associated with ESCC risk. Fasting saliva samples were collected from 87 incident and histopathologicallly diagnosed ESCC cases, 63 subjects with dysplasia and 85 healthy controls. All subjects were also interviewed with a questionnaire. V3–V4 region of 16S rRNA was amplified and sequenced by 454-pyrosequencing platform. Carriage of each genus was compared by means of multivariate-adjusted odds ratios derived from logistic regression model. Relative abundance was compared using Metastats method. Beta diversity was estimated using Unifrac and weighted Unifrac distances. Principal coordinate analysis (PCoA) was applied to ordinate dissimilarity matrices. Multinomial logistic regression was used to compare the coordinates between different groups. ESCC subjects had an overall decreased microbial diversity compared to control and dysplasia subjects (P
Oral Microbiota and Risk for Esophageal Squamous Cell Carcinoma in a High-Risk Area of China Xingdong Chen1,2,3, Björn Winckler1, Ming Lu3,4*, Hongwei Cheng5, Ziyu Yuan2,3, Yajun Yang2,3, Li Jin2,3, Weimin Ye1,3
a11111
1 Department of Medical Epidemiology and Biostatistics, Karolinska Institutet, Stockholm, Sweden, 2 Ministry of Education Key Laboratory of Contemporary Anthropology and State Key Laboratory of Genetic Engineering, Collaborative Innovation Center for Genetics and Development, School of Life Sciences, Fudan University, Shanghai, China, 3 Fudan-Taizhou Institute of Health Sciences, Taizhou, China, 4 Clinical Epidemiology Unit, Qilu Hospital of Shandong University, Jinan, China, 5 Taixing People’s Hospital, Taixing, China * [email protected]
Abstract OPEN ACCESS Citation: Chen X, Winckler B, Lu M, Cheng H, Yuan Z, Yang Y, et al. (2015) Oral Microbiota and Risk for Esophageal Squamous Cell Carcinoma in a HighRisk Area of China. PLoS ONE 10(12): e0143603. doi:10.1371/journal.pone.0143603 Editor: Qing-Yi Wei, Duke Cancer Institute, UNITED STATES Received: September 4, 2015 Accepted: November 6, 2015 Published: December 7, 2015 Copyright: © 2015 Chen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability Statement: All relevant data are within the paper and its Supporting Information files. Funding: This work was supported by a grant from Natural Science Foundation of China (81072358, 81273151), and a grant from Science and Technology Committee of Shandong Province (2012GSF11857). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Poor oral health has been linked with an increased risk of esophageal squamous cell carcinoma (ESCC). We investigated whether alteration of oral microbiota is associated with ESCC risk. Fasting saliva samples were collected from 87 incident and histopathologicallly diagnosed ESCC cases, 63 subjects with dysplasia and 85 healthy controls. All subjects were also interviewed with a questionnaire. V3–V4 region of 16S rRNA was amplified and sequenced by 454-pyrosequencing platform. Carriage of each genus was compared by means of multivariate-adjusted odds ratios derived from logistic regression model. Relative abundance was compared using Metastats method. Beta diversity was estimated using Unifrac and weighted Unifrac distances. Principal coordinate analysis (PCoA) was applied to ordinate dissimilarity matrices. Multinomial logistic regression was used to compare the coordinates between different groups. ESCC subjects had an overall decreased microbial diversity compared to control and dysplasia subjects (P
