BiorhOni,c, c.xtochlonlc I+ or :\11 ~ synlhasc, a l t h o u g h on t h e basis o : an cnd-pt+int titration +,,ith ( ' A I . an a p p a r e n t incrcar, c is o b s c l ~ c d in the Zl!llOtilll o f ;.ldCalill+.." n L I c l c o I i d c
II-atlMOC;ASC.
Materials
(.'ollagcw.,.sc. carbcqliv dlth.vdi-asc iliad ClIZVIllCS nccc'.s:,,T for the assa.v o f nlct;,bolitcs ~ c r c o b t a i n e d frolll I A o c h r i n g c r - M a n n h c i m I(;cnmarL~ L as . ~ x bovh~c SCl'UIll alb,.min ( f r a c t i o n V ) ~ hich ~ as tictatt~ d b.~ the nlct hod o f C h c n [25]. I:('('1 ) c a m e from A l d r i c h ( ' h e t l l i c a l ( ' o . (.U.S.A.). atld atltinlycin, oligotll.x.;n, l o t c n t ) l l c , car[',oxyatrdcl.vh~sidc. 3 . 3 ' . 5 - t r i i o d o - t - t h ~ r o n i n c and palmitic acid ~ c r c pLIl'Ch;.t~¢d f r o l l l .~i~111;.I ( ' h c n l i c a l ( ' o . t LLS.A.). I'almitic acid was n c u t r a h e c d a n d d i s s o h cd in 0.15 M N a ( ' l c o n t a i n i n g t),; (~ :,, ) bo~ inc s c r u n l albumhl. \ V a t c r - i n s o l u l q c inhibitors ~ c : c di.,,sol~cd in acclOl'C alld d i s p e n s e d as r c q u h c d iiltO dl~. "vessels. T h e ;.ic~2t¢'q++t.2 ~A,;.IS thcll allo~ ~1 to cvapor~_ltc ~.lTld tilt- dF3 rcM.luc ~ a s dixsol~ cd in , +, ml o f tl. 15 M N a ( ' l c o n t a i n ing tv,; (~,~., ,.) bo~. illC :.-c. ;.till a l b u m i n (~ ith o l - ~ ithout S m M ~ a l l n i t a l c ) | , c f ~ l . . otht'F ;Itlll~2OLlS additiom, ~cl-c Ill:iCe. ( ) t l l c r chcmical~ ~,.?,," ,~1 the highest qualit.'+ c o m m , ' r c i a l l y a~ ailablc. Methods
l i~pcrthvr~idi~,m ~a,, i n d u c e d in m a l e :
. \Vis-
,,:- l-t~i~ { tS(l_ I'~t) ,,i b~,' ]'o[lr dail~ ~,vr-." njcct~t~ia~ iu the ingt, in,d region, o f 171) r ~lass. ;14d tilt." l;.tl.~ ;~,cl'c r a s e d f o r c x | 3 e r i t n c n ~ , ,,., t n c 5 t h +.[;it} i.i[[Cl conlnlcnct2111Cllt o1 ;idllliltistrzlliOll o f I';. l h c
1~ solution ~a:, p r e p a r e d by di~,>ol~ing 1 nag I ~ in 511 #1 0.1 NI NaOt-t a n d diluting this s o l u t i o n tc 2 ml with
().~Y; (~ /~) NaCI. t l y p o l h y r t f i d rats ~vcue o b t a i n e d by Ih~.rt~id-1)arath.xroidcctonly o f .s,(ltlll~, i;llS vvhcn tilt." :|ninlals had a b o d y nl~lS% of 131)-Ih0 g. T h e y w e r e t h e n kept tk+, at Ica.,,t h x~¢cks. ,tltct w h i c h t h e i r b o d y m a s s ++as 220-2hll g a n d p l a s m a T+ a n d i+ c o n c e n t r a t i o n s x+ct+c ,. I}.5 n M a n d
II-atlMOC;ASC.
Materials
(.'ollagcw.,.sc. carbcqliv dlth.vdi-asc iliad ClIZVIllCS nccc'.s:,,T for the assa.v o f nlct;,bolitcs ~ c r c o b t a i n e d frolll I A o c h r i n g c r - M a n n h c i m I(;cnmarL~ L as . ~ x bovh~c SCl'UIll alb,.min ( f r a c t i o n V ) ~ hich ~ as tictatt~ d b.~ the nlct hod o f C h c n [25]. I:('('1 ) c a m e from A l d r i c h ( ' h e t l l i c a l ( ' o . (.U.S.A.). atld atltinlycin, oligotll.x.;n, l o t c n t ) l l c , car[',oxyatrdcl.vh~sidc. 3 . 3 ' . 5 - t r i i o d o - t - t h ~ r o n i n c and palmitic acid ~ c r c pLIl'Ch;.t~¢d f r o l l l .~i~111;.I ( ' h c n l i c a l ( ' o . t LLS.A.). I'almitic acid was n c u t r a h e c d a n d d i s s o h cd in 0.15 M N a ( ' l c o n t a i n i n g t),; (~ :,, ) bo~ inc s c r u n l albumhl. \ V a t c r - i n s o l u l q c inhibitors ~ c : c di.,,sol~cd in acclOl'C alld d i s p e n s e d as r c q u h c d iiltO dl~. "vessels. T h e ;.ic~2t¢'q++t.2 ~A,;.IS thcll allo~ ~1 to cvapor~_ltc ~.lTld tilt- dF3 rcM.luc ~ a s dixsol~ cd in , +, ml o f tl. 15 M N a ( ' l c o n t a i n ing tv,; (~,~., ,.) bo~. illC :.-c. ;.till a l b u m i n (~ ith o l - ~ ithout S m M ~ a l l n i t a l c ) | , c f ~ l . . otht'F ;Itlll~2OLlS additiom, ~cl-c Ill:iCe. ( ) t l l c r chcmical~ ~,.?,," ,~1 the highest qualit.'+ c o m m , ' r c i a l l y a~ ailablc. Methods
l i~pcrthvr~idi~,m ~a,, i n d u c e d in m a l e :
. \Vis-
,,:- l-t~i~ { tS(l_ I'~t) ,,i b~,' ]'o[lr dail~ ~,vr-." njcct~t~ia~ iu the ingt, in,d region, o f 171) r ~lass. ;14d tilt." l;.tl.~ ;~,cl'c r a s e d f o r c x | 3 e r i t n c n ~ , ,,., t n c 5 t h +.[;it} i.i[[Cl conlnlcnct2111Cllt o1 ;idllliltistrzlliOll o f I';. l h c
1~ solution ~a:, p r e p a r e d by di~,>ol~ing 1 nag I ~ in 511 #1 0.1 NI NaOt-t a n d diluting this s o l u t i o n tc 2 ml with
().~Y; (~ /~) NaCI. t l y p o l h y r t f i d rats ~vcue o b t a i n e d by Ih~.rt~id-1)arath.xroidcctonly o f .s,(ltlll~, i;llS vvhcn tilt." :|ninlals had a b o d y nl~lS% of 131)-Ih0 g. T h e y w e r e t h e n kept tk+, at Ica.,,t h x~¢cks. ,tltct w h i c h t h e i r b o d y m a s s ++as 220-2hll g a n d p l a s m a T+ a n d i+ c o n c e n t r a t i o n s x+ct+c ,. I}.5 n M a n d
