.) 1990

Nucleic Acids Research, Vol. 18, No. 23 7155

Oxford University Press

Nucleotide sequences of two tRNA Micrococcus luteus

gene

clusters from

Ryoji Ikeda*, Takeshi Ohama, Akira Muto and Syozo Osawa Department of Biology, School of Science, Nagoya University, Chikusa-ku, Nagoya 464-01, Japan EMBL accession

Submitted October 29, 1990

X55099, X55100

ACKNOWLEDGEMENT

Two Pstl fragments (2.4 and 4.4 kb) from Micrococcus luteus DNA, that hybridized with the 32P-labeled total tRNA, were cloned and sequenced. The 2.4 kb DNA (MLTROOl) contained a gene cluster for four tRNAs, Gly(GCC)-Cys(GCA)-Val(GAC)Gly(GCC). The 4.4 kb DNA fragment (MLTRO02) contained two tRNA genes for Thr(GGU) and Met(CAU). Each cluster is preceded by a putative promoter structure and followed by a probable termination signal. The 3'-terminal CCA only for one of the Gly tRNA genes in MLTROO1 is coded in DNA. The first nucleotide of the T ,6C-loop (position 54) of both Thr and Met tRNAs in MLTRO02 is A, whereas the corresponding nucleotide of most of the known tRNAs is T (1). (1) MLTRO01

nos

Supported by grants from the Ministry of Education, Science and Culture of Japan.

REFERENCE 1. Sprinzl,M., Hartmann,T., Meissner,F., Moll,J. and VorderwulbeckeT. (1987) Nucl. Acids Res. 14, 5223-5232.

Gly(GCC)1-Cys(GCA)-Val(GAC)-Gly(GCC)2

;,CATCCGGGCGGAGATGGCCTAGAGATCCTTCTCGTTGGAAAACGCAGGTGAGCGGCTCAGACGAGCGGATCCCGGCGGCACCGACAC GCGGATGTAGCTCAGTTGGCTAGAGCGCCACCTTGCCAAGGTGGAGGTCGCGAGTTCGAATCTCGTCATCCGCTCGCTCAACCTTCACCCCTGGTCGGGT GACGCCGATT

100

200

Gly

GGCCGAGAGGCGAGGCAGCGGCCTGCAAACCGTATACGCGGGTTCGAA-TCCCGTCCCGACCTCGCAGGCTTCTCCGACCGGGAGTGGCGGCATGTTCAC Cys

300

CACCTCCTCGGAGGTGGACGAGCGCG TTGGCGCAGCGGTAGCGCGCTTCCCTGACACGGAAGAGGTCACTGGTTCGAACCCAGTATCGCGCACGGAGAC Val

400

500

Gly CGCGAAGGGCCCCTCTAGGsGaCTTCGTCATTCCGGGGCGCACCCGCCCGGGTGGCGAGGCCCGCCTCGGCGACGAGGGGCCGGGCAG ACGT (2) MLTRO02

600

Thr(GGU)-Met(CAU)

GCCGATTTTGCACGGTCCCGGGGCCCTGG GGCTCACCTCGTTGCCCCGATAGCTCAGTGGTAGAGCGCCATCTTGGTAAGATGGAGGTCCCGGGA

100

TCGATTCCCGGTCGGGGCwTCTGACATGAGAGCCCGCCCTCGCGGCGTGAAACCGTGAGTGCGGGCGTCTCGTGTCGTGGCGGCGTAGCTCAGCTGGTTA

200

GAGCGCACGACTCATAATCGTGAGGTCGGGGGATCGAGTCCCCCCGCCGCTI&CGGAATCGGACGGACCCGGTCACCTC,;TCG=GAGGGGCCGGGTCCGGGC

300

Thr

Met

Figure 1. Putative promoter and terminator sequences

are shown

by double underlines and

*Present address: Department of Biosciences, Teikyo University, Toyosato-dai

I

arrows,

respectively.

1, Utsunomiya, Ibaragi 320, Japan