1900 Nucleic Acids Research, Vol. 18, No. 7
.=/ 1990 Oxford University Press
Nucleotide sequences of the variable regions of monoclonal antibody MRK16
a mouse
Hirofumi Hamada, Kayoko Maezawa and Takashi Tsuruo Cancer Chemotherapy Center, Japanese Foundation for Cancer Research, 1-37-1 Kami-ikebukuro, Toshima-ku, Tokyo 170, Japan Submitted February 24, 1990
MRK16 is a mouse monoclonal antibody generated against the multidrug transporter P-glycoprotein (1), which is widely used for the diagnosis and experimental therapeutics of multidrugresistant tumors. Full-length kappa and heavy chain cDNA clones were isolated from a MRK16-producing myeloma cDNA library screened respectively with the correspondent variable region genomic DNA probes (2). Nucleotide sequences of the variable region genes of the kappa (A) and heavy (B) chain of MRK16 were determined. The N-terminal twenty amino acids of the
EMBL accession nos X51720, X51721
purified kappa and heavy chain proteins were sequenced and found to be identical with the predicted amino acid sequences (which are indicated by underlines).
REFERENCES 1. Hamada,H. and Tsuruo,T. (1986) Proc. Natl. Acad. Sci. USA 83, 7785-7789. 2. Hamada,H. (1990) Cancer Res. in press.
A CTCAGGTTGCCT CCTCAAA ATG AAG TTG CCT GTT AGG CTG TTG GTG CTG ATG TTC TGG w F M L V L L R P V L K M AGT TCT GTC CTG TCC GTC ACT CCA ACC CAA ATG TTG GTT AGT GAT AGC TCC GCT V S T S T L p L M V S D S A S GAT CAA GCC TCC ATC TCT TGC AGA TCT AGT CAG AGC ATT GTA CAT AGT ACT GGA T G V H S I S S R S C S A _ I Q D TAC ATC CTG TAT TTA GAA TGG TAC CTG CAG AAA CCA GGC CAG TCT CCA AAG CTC y I L K L p S G w Y L E Q L y Q K p TCC AAC CGC TTT TCT GGG GTC CCA GAC AGG TTC AGT GGC AGT GGA TCA GGG ACA T G S G F G S R S D V p S G R F N S ACA CTC AAA ATC AGC AGA GTG GAG GCT GAG GAT CTG GGA GTT TAT TAC TGC TTT F y V y C D L G E A V E R I S K T L AAA C ATC GAA AAG CTG ACC GGA GGC GGT TTC TTT ACG CGG CCT TCA CAT E I K L K T G T F G G R p F H S
ATT I CTT L AAT N AAA K GAT D
CCT p GGA
G ACC
T ATT I TTC F CAA GCT A Q
B GGAGGCAGGTCCTAGATTTGAGTTCCTCACATTCAGTGATCAGCACTGAACACGGAACCCTCACC ATG AAC TTT N F M GGG CTG AGC L S G GTG GAG TCT E TCT GGA TTC F S G CTG GAG TGG w L E AAG GGT CGA K R G AGT CTG AGG S L R TGG TTT GCT w F A
ATT TTA AAA GGT GTC V K I L G CCT GGA GGG TCC CTG L G G p ATG TCT TGG GTT CGC w V M R S GGT GGT GGT AAC ACC N V T G G G TTC ACC ATC TCC AGA GAC AAT GCC AAG AAC AAC N R D N A K N F S T I TCT GAG GAC ACG GCC TTG TAT TAC TGT GCA AGA T R S E D A L y y A C TCC TGG GGC CAA GGG ACT CTG GTC ACT GTC TCT w S V V T S T L G G Q
TTG L GGG G ACT T GTC
ATT TTC CTT GTC CTA V L F L I GGA GGC TTA GTG AAG K L V G G TTC AGT AGC TAT ACC y T F S S GCA ACC ATT AGT AGT A T I S S
TGT GAA GTG ATA V I CTC TCC TGT GCA OT. S A ACT CCG GAG AAG T p E K TAT CCA GAC AGT y y D p S CTG TAC CTG CAA ATG
CAG Q AAA K CAG Q TAC
CTG -IL GCC A AGG R GTG V AGC
y L L S Q M TAC TAT AGG TAC GAG GCC y
GCA G A
y
R
y
E
A
.=/ 1990 Oxford University Press
Nucleotide sequences of the variable regions of monoclonal antibody MRK16
a mouse
Hirofumi Hamada, Kayoko Maezawa and Takashi Tsuruo Cancer Chemotherapy Center, Japanese Foundation for Cancer Research, 1-37-1 Kami-ikebukuro, Toshima-ku, Tokyo 170, Japan Submitted February 24, 1990
MRK16 is a mouse monoclonal antibody generated against the multidrug transporter P-glycoprotein (1), which is widely used for the diagnosis and experimental therapeutics of multidrugresistant tumors. Full-length kappa and heavy chain cDNA clones were isolated from a MRK16-producing myeloma cDNA library screened respectively with the correspondent variable region genomic DNA probes (2). Nucleotide sequences of the variable region genes of the kappa (A) and heavy (B) chain of MRK16 were determined. The N-terminal twenty amino acids of the
EMBL accession nos X51720, X51721
purified kappa and heavy chain proteins were sequenced and found to be identical with the predicted amino acid sequences (which are indicated by underlines).
REFERENCES 1. Hamada,H. and Tsuruo,T. (1986) Proc. Natl. Acad. Sci. USA 83, 7785-7789. 2. Hamada,H. (1990) Cancer Res. in press.
A CTCAGGTTGCCT CCTCAAA ATG AAG TTG CCT GTT AGG CTG TTG GTG CTG ATG TTC TGG w F M L V L L R P V L K M AGT TCT GTC CTG TCC GTC ACT CCA ACC CAA ATG TTG GTT AGT GAT AGC TCC GCT V S T S T L p L M V S D S A S GAT CAA GCC TCC ATC TCT TGC AGA TCT AGT CAG AGC ATT GTA CAT AGT ACT GGA T G V H S I S S R S C S A _ I Q D TAC ATC CTG TAT TTA GAA TGG TAC CTG CAG AAA CCA GGC CAG TCT CCA AAG CTC y I L K L p S G w Y L E Q L y Q K p TCC AAC CGC TTT TCT GGG GTC CCA GAC AGG TTC AGT GGC AGT GGA TCA GGG ACA T G S G F G S R S D V p S G R F N S ACA CTC AAA ATC AGC AGA GTG GAG GCT GAG GAT CTG GGA GTT TAT TAC TGC TTT F y V y C D L G E A V E R I S K T L AAA C ATC GAA AAG CTG ACC GGA GGC GGT TTC TTT ACG CGG CCT TCA CAT E I K L K T G T F G G R p F H S
ATT I CTT L AAT N AAA K GAT D
CCT p GGA
G ACC
T ATT I TTC F CAA GCT A Q
B GGAGGCAGGTCCTAGATTTGAGTTCCTCACATTCAGTGATCAGCACTGAACACGGAACCCTCACC ATG AAC TTT N F M GGG CTG AGC L S G GTG GAG TCT E TCT GGA TTC F S G CTG GAG TGG w L E AAG GGT CGA K R G AGT CTG AGG S L R TGG TTT GCT w F A
ATT TTA AAA GGT GTC V K I L G CCT GGA GGG TCC CTG L G G p ATG TCT TGG GTT CGC w V M R S GGT GGT GGT AAC ACC N V T G G G TTC ACC ATC TCC AGA GAC AAT GCC AAG AAC AAC N R D N A K N F S T I TCT GAG GAC ACG GCC TTG TAT TAC TGT GCA AGA T R S E D A L y y A C TCC TGG GGC CAA GGG ACT CTG GTC ACT GTC TCT w S V V T S T L G G Q
TTG L GGG G ACT T GTC
ATT TTC CTT GTC CTA V L F L I GGA GGC TTA GTG AAG K L V G G TTC AGT AGC TAT ACC y T F S S GCA ACC ATT AGT AGT A T I S S
TGT GAA GTG ATA V I CTC TCC TGT GCA OT. S A ACT CCG GAG AAG T p E K TAT CCA GAC AGT y y D p S CTG TAC CTG CAA ATG
CAG Q AAA K CAG Q TAC
CTG -IL GCC A AGG R GTG V AGC
y L L S Q M TAC TAT AGG TAC GAG GCC y
GCA G A
y
R
y
E
A
