©) 1990 Oxford University Press 657

Nucleic Acids Research, Vol. 18, No. 3

Nucleotide sequence of the sporulation gene spoliGA from Bacillus subtilis Esteban S.Masuda, Hiroyuki Anaguchi, Tsutomu Sato1, Michio Takeuchi' and Yasuo Kobayashi Faculty of Applied Biological Science, Hiroshima University, Higashi Hiroshima 724 and 'Laboratory of Enzymology and Microbial Chemistry, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183, Japan Submitted January 9, 1990

EMBL accession no. X17344*

The activation of sporulation-specific genes in Bacillus subtilis is in part due to the modification of RNA polymerase by the association of different sigma factors. The spoIIGB gene encodes for an inactive precursor protein which is processed to form the sporulation-specific sigma factor, sigma-E (1,2). This processing is thought to be carried out by the product of the spoIIGA gene (3). We report here the nucleotide sequence of the spoIIGA gene. We suggest that its product is a membrane protein with five

membrane-spanning regions (boxed) and that its C-terminal half shares some characteristics with serine proteases (underlined).

REFERENCES 1. Trempy,J.E. et al. (1985) Proc. Natl. Acad. Sci. USA 82, 4189-4192. 2. LaBell,T.L. et al. (1987) Proc. Natl. Acad. Sci. USA 84, 1784-1788. 3. Stragier,P. et al. (1988) Cell 52, 697-704.

GATCGTCCGAGATGATTATGGAAATGAAACAAGAAAAACTGCGAATGGAAAACTTAATATGAACACAGAAAATTAAGTGGAAAAAAAGCTGCGTCATT;_ I V R D D Y G N E T R K T A N G K L N M N T E N

100

*

GCAG(-TTTTTTTATATCCTCTCATTATACTTCCTCGACAAATTAAGCAGATTTCCCTGAAAAATTGTATTTTCCTCTCAACATTAATTGACAGACTTTCC

200

1

CAGATGTGAAAATCTATTTAGATGTCATTTGGCTG M K I Y L. D IV I W L

300

TTAAACTTTTGTTTTGATGCACTTTTGCTTTTGCTCACGGCATTTATTTTAAAACGGCATGTAAAAAAAAGAAGACTGGTAGGCGGAGCGTTCATC'GGTT L N F C F D A L L L L L T A F L K R H V K K R R IL V G G A F I

400

11

CCAGTATTGTCCTTTTGATGTTTACTCCTTTTTCGCCGATTGTTGAACATCCGGCTGGGAAGCTAGCTTTTTCAGTTGTGATTGTGGTGGTGACATTTGG

Soo

A~AAG

CACAGAGCTTGCTTTATACTTATGAAGCAAGAAGGGGAACAGCGTGAGGCAAGA

S

44 -

I

L

V

L

F

M

T

SI P

F

P

I

V

E

H

rP

A

G

K

L

A

F

S

V

V

V

T

V

V

F

G

(-TTTAAAA(,ATTTCGGTTTTTCTTTCAAAATTTGTTTTCCTTTTATTTTGCCACTTTTTTAATGGGGGGAGGAATTATCGGAGCCCATTCTTTGCTGCAG 1GAl H S L L Q m K R F R F F F Q N IL F S F Y F A T F L M G G G

600

78

TCAAATTCTATT(,TTCAAAACGGTGTCATGATTACCAATCAAACAGGGTTTGGAGACCCGATCAGCTGGTTGTTTATTGTTG(,TGGCTTTCCAGCATTAT S N S V Q N G V M I T N Q T G F G D IP S W L F I V G G F P A L

700

111

(GGTT TT TTTCT AAAAGAAGAATTGAAGAT ATTGAAACAAAAAACATTCAATATGAGGAACGAGTCAGCGTGCAGGCAGATTTGGGCAGCCAAACACTTCA F FS K RR I E D I E T K N 1 Q Y E E R V S V Q A D L G S Q T L H

80 0

T GTCAGAGGCCTGAT TGATTCCGGT AACCAGCTGTACGATCCTCTTACAAAAACACCGGTAATGATCATTT ACATTGAT AAACTGGAGCCGATTTTC(iGA V R G L I D S G N Q L Y D P L T K T P V M I I Y I D K L E P I F G

90 0

A(C'AGCC-GAAACGATGATCATTCGAAACACAGACCCATTGGAAGCCATCGAACAGCTCGATGATTCCTTTC(iCTTTTTAGAT AAAATGAGGTTGATT(C-CAT

1000

144 W

178 211

T

E

I

M

I

N

R

T

D

P

L

E

A

I

E

Q

L

D

D

S

F

R

L

F

M

I P' ACAGGGGAGTTGGT CAGCAAAATCAATTTTT ATTATGCGTTAAACCGGATCACGTAACGATTATGACAAAAGAAGAAATGATTTCTGCAGAT AAATGTTT 244 Y R G V G Q Q N Q F L L C V K P D H V T I M T K E E M I S A D K C L

278

A

T

D

K

R

L

1 10 0

A AT CGGT ATCAGCACAACAAAGCTGTCGGCAGATGGAGAGTTTGACGCGATTATTCATCCGAAAATGCTTTCGGGCAAGGCTGT(:AAACACGTTTCAT AA I H P K M L S G K A V K H V S * 1 G I S T T K L S A D G E F D A 1

12'0O0

GGAAGATGAAAAAACTGAAATTACGGTTGACGCACCTCTGGTA M K K L K L R L T H L W Y

1300

TGTTCGCAAATGTCCGTTACTTATCATACTCAAACGGCGTGACATT

T

TAAGCTGCT(iATGAAACTTGGGCTGAAAAGTGATGAAGTCTATTACATAGGCGGGAGTGAAGCCCTGCCGCCTCCATTATCTAAAGATGAGGAGCAGGTT L

L

K

K

P1

L

G

L

K

TTGTTAATGAAGCTCCCAAACGGCGATC L

L

M

K

*DDBJ accession no. D00433

L

P

N

G

D

S

D

E

1428

V

Y

Y

I

G

G

S

E

A

L

P

P

P

L

S

K

D

E

E

Q

V

1400