2176 Nucleic Acids Research, Vol. 18, No. 8
© 1990 Oxford University Press
Nucleotide sequence of the PHO81 gene involved in the regulation of the repressible acid phosphatase gene in Saccharomyces cerevisiae Thierry Coche, Deborah Prozzi, Michele Legrain', Franqois Hilgerl and Jean Vandenhaute* Laboratoire de Gen6tique Moleculaire, Facultes Universitaires Notre-Dame de la Paix, B-5000 Namur and 'Unite de Microbiologie, Faculte des Sciences Agronomiques, B-5800 Gembloux, Belgium Submitted March 16, 1990 Expression of the repressible acid phosphatase gene PH05 in Saccharomyces cerevisiae involves a complex network of regulatory elements, composed of at least 5 gene products: PHO2, PHO4, PHO80, PHO81 and PHO85 (1). The PHO81 gene was isolated on a 7 kilobase BamHI-Sall clone by complementation of a pho8l mutant (2). The sequence of a 4191 base pair (bp) SpeI-HpaI fragment bearing the PHO81 gene is presented here. The nucleotide sequence contains one large 3531 bp open reading
EMBL accession no. X52482 frame spanning bases 127 to 3657. Discrepancies between the sequence data and results presented by Yoshida et al. (1) have been noticed. REFERENCES 1. Yoshida,K., Ogawa,N. and Oshima,Y. (1989) Mol. Gen. Genet. 217, 40-46. 2. Vandenhaute,J., Legrain,M., Coquette,V., Tibor,A. and Hilger,F. (1988)
Arch. Int. Physiol. Bioch. 96, Bi 16.
1
ACTAGTTGAGCTTTTGACAAGACATACTGCTCAAAAAATCTTCATAACATTATTTTTCGGTTCCACAGTGATTGAGCTTTTTGAGAGAATAACCCTTTGG
101 201 301 401 501 601 701 801 901 1001
AGGCAACATAGATAGATAAACGTGCAATGAAATTCGGCAAGTATTTGGAAGCCAGGCAGTTGGAACTGGCGGAGTACAACAGCCATTTTATTGATTATAA GGCTCTAAAGAAACTTATCAAACAATTGGCCATTCCACGCTAAAGGCTAGTTCAGATTTAGATTTGCATTTAACATTAGATGACATCGACGAAAAAATT ATACATCAGAGACTGCAAGAAAATAAAGCTGCCTTTTTTTTTAAACTAGAGAGGGAGCTGGAAAAAGTAAATGGTTATTATTTGGCGAGAGAGTCCGATT TAAGAATAAAATTCAATATATTACACTCAAAATATAAAGATTATAAGATAAATGGTAAGCTAAACTCAAACCAAGCTACCTCATTTAAAAACTTATATGC GGCTTTCAAAAAGTTTCAAAAGGATCTTAGAAATTTGGAGCAGTATGTTGAGTTAAATAAAACGGGATTTTCAAAAGCTCTGAAGAAATGGGACAAGAGA TCTCAATCTCACGATAAAGATTTTTACCTTGCTACTGTTGTTTCCATTCAACCAATTTTTACCAGAGATGGGCCACTAAAATTAAACGATGAAACTTTAC ATATTCTCTTAGAGCTGAATGATATTGACAACAACAATAGAAGGGCAGACATACAATCCAGTACGTTTACTAATGACGATGATGACGACAATAATACCAG CAACAATAATAAACACAATAATAATAATAACAATAATAATAACAACAACAACAACAACAATAATAATAACATATTACACAATAATTATGAATTAACAACA TCAAAGATTTCGGAAAACCAACTTGAACATCTTTTTCAAGCATCTTCCTCCTCCTTGGACATGGAGATGGAAATCGAAAACTGGTATAAGGAAATTTTGA ATATAGCCACGGTGAAAGATGTTCAAAGAAAGCATGCACTACTAAGAAACTTCAGAGAAACCAAGATATTCACATATCTGCTACAAAATTCCTCAGAATC TTTTCATAAAAATGTCTTTTCTTTGTTGAAGGAGTGCTTAACGACGCTGTTTTTATTACTGGTGGCTAGTCCTTTGGACGATAACTCTCTGCATATTTTC TATAAAAGCAATCAGGATCATATTGATCTGTCATATTGTGACGAAGATGATCAAGTATTTTCACGAAAAAACGTATTTCACGAGGCTGCAAGTTGTCCAG AAAAATCACGCTTATTTATTCTAGATGAGGCTTTAACAACATCAAAGTTATCTAAAGAAACAGTACAAAAACTACTGAATGCCCAAGATATACATTCGCG GGTGCCGCTACATTATGCTGCAGAGCTAGGAAAACTGGAATTTGTTCACTCGTTGCTAATCACCAATCTTTTAGAGGACGTTGATCCCATTGATAGTGAT TCTAAGACACCCTTGGTTTTGGCTATCACTAACAACCACATAGATGTTGTTAGGGATCTGCTGACTATTGGTGGTGCGAACGCATCACCCATTGAAAAGC CAATTCTGGATTATAGTAAGAATGTAATCAGCTCTACTAAAGTCCAGTTCGATCCTTTAAATGTTGCTTGTAAATTTAATAATCATGACGCCGCCAAGTT ACTTTTAGAAATTCGGAGTAAGCAAAATGCTGATAATGCCAAGAACAAAAGTTCACAACACTTGTGTCAACCGCTTTTCAAAAAGAACTCAACAGGTTTA TGCACTCTTCATATAGTGGCAAAAATTGGCGGCGATCCACAATTAATTCAACTATTGATACGTTATGGGGCGGATCCCAACGAAATTGATGGGTTTAATA AATGGACGCCCATTTTTTACGCTGTTCGTTCAGGTCATTCTGAAGTTATTACTGAACTATTGAAACATAATGCACGTCTGGATATTGAAGATGACAACGG CCATTCGCCACTTTTTTACGCCTTATGGGAGAGCCACGTTGATGTTTTGAATGCACTTTTACAAAGACCATTAAATTTGCCATCTGCACCCCTGAATGAA ATAAATTCGCAGTCTAGTACGCAACGCCTTAATACAATAGATTTAACCCCAAATGATGACAAATTTGATTTAGACATTCAAGATAGCATTCCGGATTTTG CTTTACCACCGCCAATCATTCCACTAAGGAAATATGGTCATAATTTTTTGGAGAAAAAAATTTTCATCAAATTAAAGTTGAGGCCAGGTCTCGAGTCTAT CAAGTTGACTCAGGATAACGGCATTATTATGTCATCATCACCAGGCAGAATTACTTTATCCTCAAACTTACCTGAAATAATACCGCGAAATGTTATTTTA CCTGTTAGATCTGGCGAAATTAATAACTTTTGTAAGGATATCAGTGAAACAAATGATGAAGAAGATGATGATGAAATTAGTGAAGATCATGATGATGGA
1101 L201 1301 1401 1501
1601 1701 1801 1901 2001 2101 2201 2301 2401 2501 2601
AGATAATTTTCCAAGTAGATTCAATCGACGATTTTTCAATGGATTTCGAGATATTTCCTTCATTTGGCACAAGGATAATTGCCAAAACAACGGCAATGCC ATTCCTTTTCAAGAAGGTGGCAATAAATAGTATTGCAACCATGAATTTACCCTTATTCGACACAAGACTAAATAATATTG-TTCTCTCACTTTGGATTAC 2701 CAAATTATTTTTCCTTATCCGGGAAATCCACTGAAAATCATAAATTATGAGCCGTATTGGAAATCTACAGGAAGTGATTTAATGAC-TCTAGTAAAGA-G 2801 GTAATTTTGTTACTTCTTCATCGTTGAATGGCAGTTTTATTAGTGTATTAGTTTGCGCTCTGAATGATGAAACCATArTGGCGCCA,;AA\CCA-ACGT-;GA 2901 ATTCAAAGGCACGAAGATTCTGTTAAATGATTTAACGAAAGAACAATTGGAAAAGGTAGTGGATTATGACTTCGGTAAAATTGATGGAAGCTTCGATGAl"% 3001 GTAACATTGAAACAATACTTATCTTCGAGAGTGGTGCCTCTCAGAAGCCTACTAGAAGTTATTCCG-rGTCA-CCCAr CT-GTAAT.STGTATATTT'C
3101 3201 3301 3401 3501 3601 3701 3801
.3901 1001 q101
CTACAGATAA-GAAATTGACACAATTCCATCAAAATATCGCCATTTATAAATATCAATCAATTCATTGATAAGCTTTTACTAATCATrTTCGAGCATGA ACGTTTTTTGCGTCACAGCGGAAGTGGGAGTATGCGCCAAATAGTTTTCAGTTCATGCAATTGGGAAGCTTGCTCGATCCTTAACTGGAAACAACCCAAT TTTCCGGTTTTGTTGCAAATGAAAAATCTACTCAGAGATTCAACACAGGCAAATTTGTAGGTGATACTCCTAATTGTTTAAAGGAACTGGCCGTAAATC CTCAAAAAATGTCGTATTTAAACACGGAACTAATAAACATACATACAATGGTTCAATTTGCTATGAAcAATAATTTGTTAGGTGTAACGCTTCCATATGA AGTACTGAAGATATGCCCGTCGTTGGCTAGGATCATCAAACAAAACGGACTACTGTTGATTGCATCGGTCGGGGAAAATGACCAAATACCAGCTGATGGA
GGTTACAGCGGGATCTACTACGCTTGTGAGTTGCTTTTTGAGAATAATATTGATATGTAAAGTTCTGTAATATGTAGTTTTGAAATCTTATACATTATTT AGGAAAAACTTTTTAACCTGTTCATTAAGGGCAACATCTTCTGCCTTAGCGCCTTTATTATCTACAAGATCATATTCTGCACCATACTTTTCCACCAACA ATACAGCTGCGTCACCGTGCCTTCAGCCAAAGCATGAAATAGTGGGGTCCAACCTGCTTATCCTGCCAATTTACAGCGCTTTTGCCAAACCGCATAA TAGTTCAATTAATTTCAAAGAACCACCGACGCAGCTCTATGCAATGGAATTTGATTAAATTTATCTTTAATTCTTACGGACGCACCATTTTCAATTAAA AATTGGGATACTTCAAACCATTTCTTACCAACTGCCAAGTGCAAGCATGTAACTCCTTGATTGGTTATCTTATTTAGATCAGGCTTTAGAGGTCTGTCGT
AAAGAGACTTTACCACCTCCAAATTCcCCACTGAAcAAGCAATATGGAAAGGTGTCCATCCAGAATCATCTGGATAATCATCTAAGTTAAC
*To whom correspondence should be addressed at Laboratoire de Genetique Moleculaire, Facultes Universitaires Notre-Dame de la Paix, B-5000 Namur, Belgium
© 1990 Oxford University Press
Nucleotide sequence of the PHO81 gene involved in the regulation of the repressible acid phosphatase gene in Saccharomyces cerevisiae Thierry Coche, Deborah Prozzi, Michele Legrain', Franqois Hilgerl and Jean Vandenhaute* Laboratoire de Gen6tique Moleculaire, Facultes Universitaires Notre-Dame de la Paix, B-5000 Namur and 'Unite de Microbiologie, Faculte des Sciences Agronomiques, B-5800 Gembloux, Belgium Submitted March 16, 1990 Expression of the repressible acid phosphatase gene PH05 in Saccharomyces cerevisiae involves a complex network of regulatory elements, composed of at least 5 gene products: PHO2, PHO4, PHO80, PHO81 and PHO85 (1). The PHO81 gene was isolated on a 7 kilobase BamHI-Sall clone by complementation of a pho8l mutant (2). The sequence of a 4191 base pair (bp) SpeI-HpaI fragment bearing the PHO81 gene is presented here. The nucleotide sequence contains one large 3531 bp open reading
EMBL accession no. X52482 frame spanning bases 127 to 3657. Discrepancies between the sequence data and results presented by Yoshida et al. (1) have been noticed. REFERENCES 1. Yoshida,K., Ogawa,N. and Oshima,Y. (1989) Mol. Gen. Genet. 217, 40-46. 2. Vandenhaute,J., Legrain,M., Coquette,V., Tibor,A. and Hilger,F. (1988)
Arch. Int. Physiol. Bioch. 96, Bi 16.
1
ACTAGTTGAGCTTTTGACAAGACATACTGCTCAAAAAATCTTCATAACATTATTTTTCGGTTCCACAGTGATTGAGCTTTTTGAGAGAATAACCCTTTGG
101 201 301 401 501 601 701 801 901 1001
AGGCAACATAGATAGATAAACGTGCAATGAAATTCGGCAAGTATTTGGAAGCCAGGCAGTTGGAACTGGCGGAGTACAACAGCCATTTTATTGATTATAA GGCTCTAAAGAAACTTATCAAACAATTGGCCATTCCACGCTAAAGGCTAGTTCAGATTTAGATTTGCATTTAACATTAGATGACATCGACGAAAAAATT ATACATCAGAGACTGCAAGAAAATAAAGCTGCCTTTTTTTTTAAACTAGAGAGGGAGCTGGAAAAAGTAAATGGTTATTATTTGGCGAGAGAGTCCGATT TAAGAATAAAATTCAATATATTACACTCAAAATATAAAGATTATAAGATAAATGGTAAGCTAAACTCAAACCAAGCTACCTCATTTAAAAACTTATATGC GGCTTTCAAAAAGTTTCAAAAGGATCTTAGAAATTTGGAGCAGTATGTTGAGTTAAATAAAACGGGATTTTCAAAAGCTCTGAAGAAATGGGACAAGAGA TCTCAATCTCACGATAAAGATTTTTACCTTGCTACTGTTGTTTCCATTCAACCAATTTTTACCAGAGATGGGCCACTAAAATTAAACGATGAAACTTTAC ATATTCTCTTAGAGCTGAATGATATTGACAACAACAATAGAAGGGCAGACATACAATCCAGTACGTTTACTAATGACGATGATGACGACAATAATACCAG CAACAATAATAAACACAATAATAATAATAACAATAATAATAACAACAACAACAACAACAATAATAATAACATATTACACAATAATTATGAATTAACAACA TCAAAGATTTCGGAAAACCAACTTGAACATCTTTTTCAAGCATCTTCCTCCTCCTTGGACATGGAGATGGAAATCGAAAACTGGTATAAGGAAATTTTGA ATATAGCCACGGTGAAAGATGTTCAAAGAAAGCATGCACTACTAAGAAACTTCAGAGAAACCAAGATATTCACATATCTGCTACAAAATTCCTCAGAATC TTTTCATAAAAATGTCTTTTCTTTGTTGAAGGAGTGCTTAACGACGCTGTTTTTATTACTGGTGGCTAGTCCTTTGGACGATAACTCTCTGCATATTTTC TATAAAAGCAATCAGGATCATATTGATCTGTCATATTGTGACGAAGATGATCAAGTATTTTCACGAAAAAACGTATTTCACGAGGCTGCAAGTTGTCCAG AAAAATCACGCTTATTTATTCTAGATGAGGCTTTAACAACATCAAAGTTATCTAAAGAAACAGTACAAAAACTACTGAATGCCCAAGATATACATTCGCG GGTGCCGCTACATTATGCTGCAGAGCTAGGAAAACTGGAATTTGTTCACTCGTTGCTAATCACCAATCTTTTAGAGGACGTTGATCCCATTGATAGTGAT TCTAAGACACCCTTGGTTTTGGCTATCACTAACAACCACATAGATGTTGTTAGGGATCTGCTGACTATTGGTGGTGCGAACGCATCACCCATTGAAAAGC CAATTCTGGATTATAGTAAGAATGTAATCAGCTCTACTAAAGTCCAGTTCGATCCTTTAAATGTTGCTTGTAAATTTAATAATCATGACGCCGCCAAGTT ACTTTTAGAAATTCGGAGTAAGCAAAATGCTGATAATGCCAAGAACAAAAGTTCACAACACTTGTGTCAACCGCTTTTCAAAAAGAACTCAACAGGTTTA TGCACTCTTCATATAGTGGCAAAAATTGGCGGCGATCCACAATTAATTCAACTATTGATACGTTATGGGGCGGATCCCAACGAAATTGATGGGTTTAATA AATGGACGCCCATTTTTTACGCTGTTCGTTCAGGTCATTCTGAAGTTATTACTGAACTATTGAAACATAATGCACGTCTGGATATTGAAGATGACAACGG CCATTCGCCACTTTTTTACGCCTTATGGGAGAGCCACGTTGATGTTTTGAATGCACTTTTACAAAGACCATTAAATTTGCCATCTGCACCCCTGAATGAA ATAAATTCGCAGTCTAGTACGCAACGCCTTAATACAATAGATTTAACCCCAAATGATGACAAATTTGATTTAGACATTCAAGATAGCATTCCGGATTTTG CTTTACCACCGCCAATCATTCCACTAAGGAAATATGGTCATAATTTTTTGGAGAAAAAAATTTTCATCAAATTAAAGTTGAGGCCAGGTCTCGAGTCTAT CAAGTTGACTCAGGATAACGGCATTATTATGTCATCATCACCAGGCAGAATTACTTTATCCTCAAACTTACCTGAAATAATACCGCGAAATGTTATTTTA CCTGTTAGATCTGGCGAAATTAATAACTTTTGTAAGGATATCAGTGAAACAAATGATGAAGAAGATGATGATGAAATTAGTGAAGATCATGATGATGGA
1101 L201 1301 1401 1501
1601 1701 1801 1901 2001 2101 2201 2301 2401 2501 2601
AGATAATTTTCCAAGTAGATTCAATCGACGATTTTTCAATGGATTTCGAGATATTTCCTTCATTTGGCACAAGGATAATTGCCAAAACAACGGCAATGCC ATTCCTTTTCAAGAAGGTGGCAATAAATAGTATTGCAACCATGAATTTACCCTTATTCGACACAAGACTAAATAATATTG-TTCTCTCACTTTGGATTAC 2701 CAAATTATTTTTCCTTATCCGGGAAATCCACTGAAAATCATAAATTATGAGCCGTATTGGAAATCTACAGGAAGTGATTTAATGAC-TCTAGTAAAGA-G 2801 GTAATTTTGTTACTTCTTCATCGTTGAATGGCAGTTTTATTAGTGTATTAGTTTGCGCTCTGAATGATGAAACCATArTGGCGCCA,;AA\CCA-ACGT-;GA 2901 ATTCAAAGGCACGAAGATTCTGTTAAATGATTTAACGAAAGAACAATTGGAAAAGGTAGTGGATTATGACTTCGGTAAAATTGATGGAAGCTTCGATGAl"% 3001 GTAACATTGAAACAATACTTATCTTCGAGAGTGGTGCCTCTCAGAAGCCTACTAGAAGTTATTCCG-rGTCA-CCCAr CT-GTAAT.STGTATATTT'C
3101 3201 3301 3401 3501 3601 3701 3801
.3901 1001 q101
CTACAGATAA-GAAATTGACACAATTCCATCAAAATATCGCCATTTATAAATATCAATCAATTCATTGATAAGCTTTTACTAATCATrTTCGAGCATGA ACGTTTTTTGCGTCACAGCGGAAGTGGGAGTATGCGCCAAATAGTTTTCAGTTCATGCAATTGGGAAGCTTGCTCGATCCTTAACTGGAAACAACCCAAT TTTCCGGTTTTGTTGCAAATGAAAAATCTACTCAGAGATTCAACACAGGCAAATTTGTAGGTGATACTCCTAATTGTTTAAAGGAACTGGCCGTAAATC CTCAAAAAATGTCGTATTTAAACACGGAACTAATAAACATACATACAATGGTTCAATTTGCTATGAAcAATAATTTGTTAGGTGTAACGCTTCCATATGA AGTACTGAAGATATGCCCGTCGTTGGCTAGGATCATCAAACAAAACGGACTACTGTTGATTGCATCGGTCGGGGAAAATGACCAAATACCAGCTGATGGA
GGTTACAGCGGGATCTACTACGCTTGTGAGTTGCTTTTTGAGAATAATATTGATATGTAAAGTTCTGTAATATGTAGTTTTGAAATCTTATACATTATTT AGGAAAAACTTTTTAACCTGTTCATTAAGGGCAACATCTTCTGCCTTAGCGCCTTTATTATCTACAAGATCATATTCTGCACCATACTTTTCCACCAACA ATACAGCTGCGTCACCGTGCCTTCAGCCAAAGCATGAAATAGTGGGGTCCAACCTGCTTATCCTGCCAATTTACAGCGCTTTTGCCAAACCGCATAA TAGTTCAATTAATTTCAAAGAACCACCGACGCAGCTCTATGCAATGGAATTTGATTAAATTTATCTTTAATTCTTACGGACGCACCATTTTCAATTAAA AATTGGGATACTTCAAACCATTTCTTACCAACTGCCAAGTGCAAGCATGTAACTCCTTGATTGGTTATCTTATTTAGATCAGGCTTTAGAGGTCTGTCGT
AAAGAGACTTTACCACCTCCAAATTCcCCACTGAAcAAGCAATATGGAAAGGTGTCCATCCAGAATCATCTGGATAATCATCTAAGTTAAC
*To whom correspondence should be addressed at Laboratoire de Genetique Moleculaire, Facultes Universitaires Notre-Dame de la Paix, B-5000 Namur, Belgium
