Nucleic Acids Research, Vol. 18, No. 11 3413

Nucleotide sequence of carbonic anhydrase

pea

cDNA encoding chloroplast

Chrissl A.Roeske and William L.Ogrenl * Department of Plant Biology, University of Illinois at Urbana-Champaign and 1Agricultural Research Service, United States Department of Agriculture, 1102 South Goodwin Avenue, Urbana, IL 61801, USA Submitted April 3, 1990 A 1.3 kb cDNA was isolated from a pea (Pisum sativum L.) cDNA library by immunoscreening with rabbit antiserum to spinach carbonic anhydrase (E.C. 4.2.1.1). The 984 bp open reading frame contained the entire coding region of the mature chloroplast protein and its transit peptide. The identity of the protein is verified by the strong homology with carbonic anhydrase from spinach (1). There is little sequence homology with mammalian isozymes (2). The transit peptide, based on Edman degradation of the mature polypeptide, is 104 amino acids. This is longer than commonly found for nuclear-encoded chloroplast proteins (3). Also unusual, even for the serine- and

EMBL accession

REFERENCES 1. Burnell,J.N., Gibbs,M.J. and Mason,J.G. (1990) Plant Physiol. 92, 37-40. 2. Hewett-Emmett,D., Hopkins,P.J., Tashian,R.E. and Czelusniak,J. (1984) Annals N. Y Acad. Sci. 429, 338-358. 3. Keegstra,K., Olsen,L.J. and Thea,S.M. (1989) Ann. Rev. Plant Physiol. Plant Mol. Biol. 40, 471-501.

AAGCCTACCAACTTTCATCATATGATATTTTATATATATGAGAATCTATGAACTTGTATTATGTACTATATATCATGTATCCCATCTTAAA TAAGAGTTTCTMTTCCTATTGAGGCAMMAATTTCMGAGTTTGTTCAAAAAAAMAAMAM

To whom correspondence should be addressed

X52558

theonine-rich chloroplast transit peptides, is a region of seven consecutive Ser residues bounded by Thr residues (amino acid residues 36-43). The molecular mass of the deduced 224 amino acid mature chloroplast polypeptide is 24.2 kd.

GGTTTGAATCTTCATTGCACCA ATG TCT ACC TCT TCA ATA AAC GGC TTT AGT CTT TCT TCT TTG TCC CCT GCC S P A S S S L S S L N G F I M S T AAA ACT TCT ACC AAA AGA ACT ACA TTG AGA CCC TTT GTT TCT GCA TCT CTT AAC ACT TCT TCT TCA TCT S T S S S S L N P F T V S A R T L K R T S T K TCT TCC TCG ACT TTC CCT TCT CTT ATT CAA GAC AAG CCG GTT TTC GCT TCT TCT TCT CCT ATC ATC ACC Q S S S P I I T F D K A P V I P S F SS S L T CCA GTT TTG AGA GAA GM ATG GGA AAG GGC TAT GAT GAA GCT ATT GAA GAA CTC CAA AAG TTG TTG AGG E R I E L L L Q K P V L R E E M G K G Y D E A ACA GCT GCT GAG AAG GTT GAG CM ATC GAG MG ACT GAA CTG AAA GCC ACA GCT CAG CTA GGA ACA ACA T A Q G T T I L E K T L K A T A A E K V E Q E TCA TCA TCT GAT GGC ATT CCA AAA TCT GM GCC TCT GAA AGG ATC AAA ACT GGT TTC CTT CAC TTC AAG H F K T G F I K L R A S E E P K S I G S S S D AAA GAG AAA TAT GAC MG AAT CCA GCT TTG TAT GGT GAA CTT GCC AAA GGC CAA AGC CCT CCG TTT ATG F M N A L A K G Q S P P D K P L Y G E Y K K E GTG TTT GCA TGT TCA GAC TCA AGA GTC TGC CCA TCT CAT GTG CTA GAT TTC CAG CCA GGT GAA GCC TTT E A F Q P G F V H L D S D S R V C P S F A C V GTG GTC AGA AAT GTT GCT MC TTG GTT CCA CCA TAT GAC CAG CGA AAA TAT GCC GGA ACT GGT GCT GCA G A A N N V A R L V p P Y D Q A K Y A G T V V ATT GAG TAC GCA GTT CTG CAT CTC AAG GTT TCC AAC ATT IGTT GTC ATT GGA CAC AGT GCT TGT GGT GGT I G H S A C G G I V V Y A V L H L K V S N I E ATT AAG GGA CTT TTG TCC TTT CCA TTT GAT GGA ACC TAC TCC ACT GAT TTC ATT GAG GAG TGG GTC AAA I E E W V K I K G L L S F P F D G T Y S T D F ATT GGT TTA CCT GCA AAG GCG AAG GTG AM GCA CM CAT GGA GAT GCA CCT TTT GCA GAG CTA TGC ACA L C T G I L P A K A K V K A Q H G D A P F A E CAC TGT GAG AAG GAA GCT GTG AAT GCT TCC CTT GGA MC CTT CTC ACC TAC CCA TTT GTG AGA GAG GGA V R E G F N A S L G N L L T Y P H C E K E A V TTG GTG AAC MG ACA TTG GCA CTC AM GTA GGA TAC TAT GAC TTT GTG AAA GGA TCC TTT GAG CTT TGG S F E V K G F L W L V N K T L A L K G G Y Y D GGA CTT GAA TTT GGC CTT TCG TCC ACT TTC TCC GTA TGAACATCAACCATATATCAATGACCACATCTTGATTACTAAG F S V F G G L S S T L E TATTCTTCTCCCTAAMATGTCAAAGATGTGGCCACGATTCTACATTGGAAGCTATAATCTCGTGGTTAAAGGTCGTGTGTCTGCAATGAAG

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no.

17 40 63 86 109 132

155 178 201 224 247 270 293

316 328

Nucleotide sequence of pea cDNA encoding chloroplast carbonic anhydrase.

Nucleic Acids Research, Vol. 18, No. 11 3413 Nucleotide sequence of carbonic anhydrase pea cDNA encoding chloroplast Chrissl A.Roeske and William...
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