Nucleic Acids Research, Vol. 18, No. 10 3061
Nucleotide sequence of Chlamydomonas moewusii chloroplastic tRNA Martine Richard and Guy Bellemare Departement de Biochimie, Facuite de Sciences et de Genie, Universite Laval, Quebec Gl K 7P4, Canada EMBL accession no. X51398
Submitted April 9, 1990
Following the physical mapping of Chlamydomonas moewusii chloroplastic DNA (1), we selected clones on the basis of their hybridization with positively light-regulated transcripts from the same organism (2, 3). One such clone containing a 13.5 kb insert was subcloned and a SalI-XbaI 2.4 kb fragment sequenced. It contained, among other features, a 1,689 bp ORF 563 (4) similar to ORF 513 from the chloroplastic genome of Marchantia polymorpha (5) and a (tgt) tRNAthr spanning nucleotides -602 to -528 with respect to ORF 563 initiation codon. For comparison, in M. polymorpha a similar (tgt) tRNAthr is located about 26,000 bp downstream from ORF 513, indicating evolutionary rearrangements; moreover in this organism a (ttg) tRNAgIn can be found between -245 and -176 (5).
ACKNOWLEDGEMENTS This work was supported by NSERC A6923.
REFERENCES 1. Turmel,M., Bellemare,G. and Lemieux,C. (1987) Curr. Genet. 11, 543-552. 2. Estable,M. (1986) M. Sc. Thesis, Universite Laval, Qu6bec, Canada. 3. Turmel,M., Lemieux,B. and Lemieux,C. (1988) Mol. Gen. Genet. 214, 412-419. 4. Richard,M. and Bellemare,G. (in preparation). 5. Unesomo,K., Inokuchi,H., Shiki,Y., Takeuchi,M., Chang,Z., Fukuzawa,H., Kohchi,T., Shirai,H., Ohyama,K. and Ozeki,H. (1988) J. Mol. Biol. 203, 299-331.
1
GCTTGCTTAG
CTCAATTCGG
TAGAGCTTTG
GTTTTGTAAA
41
CCAAAGGTTA
TCGGTTCAAG
TCCGATAGCA
AGCT