©D 1990 Oxford University Press
4926 Nucleic Acids Research, Vol. 18, No. 16
Nucleotide sequence of cDNA encoding the BYMV coat protein gene K.Boye*, P.E.Jensen, B.M.Stummann and K.W.Henningsen Department of Genetics, The Royal Veterinary and Agricultural University, BOlowsvej 13, DK-1970 Copenhagen F, Denmark Submitted July 13, 1990
EMBL accession
cDNA representing the 3' end of the genomic RNA of a Danish isolate of Bean Yellow Mosaic Virus (BYMV, a potyvirus) has been cloned and sequenced. The location of the coat protein gene was determined by comparison with the coat protein gene of an American BYMV isolate (1). The coding regions are 94% identical in deduced amino acid sequences (fig. 1) and 96% identical in nucleotide sequences. The 3' non-translated regions are 92.5% identical in nucleotide sequences.
no.
X53684
ACKNOWLEDGEMENTS This work was supported by the Research Centre for Plant Biotechnology, Danish Biotechnology Programme 1987-90. Virus preparation was kindly supplied by the Biotechnology Group, Danish Research Service for Plant and Soil Science. REFERENCE 1. Hammond,J. and Hammond,R.W. (1989) J. Gen. Virol. 70, 1961-1974.
60 120 TCTGACCAAGAACAACTCAATGCAGGTGAGGAGAAGAAGGATAAAAAGAAAAAGAATGAA GAAAATCCTGATAAGAACTCTGAGGGGCAGAATAGCAGGCAAATAGTACCAGACAGAGAT S D Q E Q L N A G E E K K D K K K K N E E N P D K N S E G Q N S R Q I V P D R D G
R
N
D
S V
180 240 GTGAATGCAGGAACTGTTGGAACATTTTCAGTTCCTAAGCTCAAGAAAATAGCAGGAAAA CTAAATATTCCCAAGATTGGTGGAAAGATAGTGCTCAATTTAGACCACTTGCTGGAATAT V N A G T V G T F S V P K L K K I A G K L N I P K I G G K I V L N L D H L L E Y R F K 300 360 AACCCACCACAGGATGGCATTTCAAATGTCATAGCAACACAAGTACAGTTTGAAGCGTGG TACAGTGGTGTCAAGCAAGCGTATGAGGTTGAAGATTCACAAATGGGAATTATTTTGAAT N P P Q D G I S N V I A T Q V Q F E A W Y S G V K Q A Y E V E D S Q M G I I L N A
D
N
R
420
480
GGCCTTATGGTGTGGTGCATAGAAAATGGCACATCAGGAGATTTACAAGGTGAGTGGACA ATGATGGATGGAGAGGAACAGGTGACATACCCTCTAAAACCCATCTTGGACAATGCAAAG G L M V W C I E N G T S G D L Q G E W T M M D G E E Q V T Y P L K P I L D N A K 540
600
CCAACATTCCGCCAAATAATGTCGCATTTTTCAGAAGTTGCAGAAGCCTACATTGAAAAG AGGAATGCAACAGAGAGGTACATGCCACGGTATGGACTTCAAAGGAACTTGACCGATTAT E A Y I E K R N A T E P T F R Q
I
M
S
H F S
E V A
R Y M
660
P R Y G E
L Q R N
L
T
D
Y
720
GGTTTGGCCAGGTATGCTTTTGACTTCTACAAACTGACTTCAAGAACTCCTGTGCGTGCT AGAGAAGCACACATGCAAATGAAGGCGGCAGCAGTTAGAGGCAAGTCAACTAGATTATTT G
L A
R Y A
F D
F Y K L T S E
R T
P
V
R
A
780
R
E A H M Q M K A A A V R G K S A
T
R
L
F 840
GGACTTGATGGCAATGTTGGAACAGACGAGGAGAACACAGAGAGACACACAGCAGGAGAT GTCAATCGTGATATGCACACCATGCTTGGTGTTCGTATTTAGAGTATccGTcTTTAAATT G
L D G N V G T D E
E N T E R H
T
A
G
D V N R D M H T M L G V 900
R
I
960
CTCTATAATTTGGCGTTACATTACTTAATACTATGTATTAGTGAGGTTTCACCTCCAGCA TTTTAAATTCAGTATGTGTATCATTCTCTCTACTCTGACAGGGTAAGcAGTTAGTGAGGT
1024 TGCCTCGTGTGAGCCTGATCTTTGTAGAGCGAG (A)3.
Fig. 1. Nucleotide sequence and deduced amino acid sequence of the BYMV coat protein gene. Amino acids differing in the coat protein sequence of the American BYMV isolate
*
are
shown below. The translation stop codon is underlined.
To whom correspondence should be addressed
4926 Nucleic Acids Research, Vol. 18, No. 16
Nucleotide sequence of cDNA encoding the BYMV coat protein gene K.Boye*, P.E.Jensen, B.M.Stummann and K.W.Henningsen Department of Genetics, The Royal Veterinary and Agricultural University, BOlowsvej 13, DK-1970 Copenhagen F, Denmark Submitted July 13, 1990
EMBL accession
cDNA representing the 3' end of the genomic RNA of a Danish isolate of Bean Yellow Mosaic Virus (BYMV, a potyvirus) has been cloned and sequenced. The location of the coat protein gene was determined by comparison with the coat protein gene of an American BYMV isolate (1). The coding regions are 94% identical in deduced amino acid sequences (fig. 1) and 96% identical in nucleotide sequences. The 3' non-translated regions are 92.5% identical in nucleotide sequences.
no.
X53684
ACKNOWLEDGEMENTS This work was supported by the Research Centre for Plant Biotechnology, Danish Biotechnology Programme 1987-90. Virus preparation was kindly supplied by the Biotechnology Group, Danish Research Service for Plant and Soil Science. REFERENCE 1. Hammond,J. and Hammond,R.W. (1989) J. Gen. Virol. 70, 1961-1974.
60 120 TCTGACCAAGAACAACTCAATGCAGGTGAGGAGAAGAAGGATAAAAAGAAAAAGAATGAA GAAAATCCTGATAAGAACTCTGAGGGGCAGAATAGCAGGCAAATAGTACCAGACAGAGAT S D Q E Q L N A G E E K K D K K K K N E E N P D K N S E G Q N S R Q I V P D R D G
R
N
D
S V
180 240 GTGAATGCAGGAACTGTTGGAACATTTTCAGTTCCTAAGCTCAAGAAAATAGCAGGAAAA CTAAATATTCCCAAGATTGGTGGAAAGATAGTGCTCAATTTAGACCACTTGCTGGAATAT V N A G T V G T F S V P K L K K I A G K L N I P K I G G K I V L N L D H L L E Y R F K 300 360 AACCCACCACAGGATGGCATTTCAAATGTCATAGCAACACAAGTACAGTTTGAAGCGTGG TACAGTGGTGTCAAGCAAGCGTATGAGGTTGAAGATTCACAAATGGGAATTATTTTGAAT N P P Q D G I S N V I A T Q V Q F E A W Y S G V K Q A Y E V E D S Q M G I I L N A
D
N
R
420
480
GGCCTTATGGTGTGGTGCATAGAAAATGGCACATCAGGAGATTTACAAGGTGAGTGGACA ATGATGGATGGAGAGGAACAGGTGACATACCCTCTAAAACCCATCTTGGACAATGCAAAG G L M V W C I E N G T S G D L Q G E W T M M D G E E Q V T Y P L K P I L D N A K 540
600
CCAACATTCCGCCAAATAATGTCGCATTTTTCAGAAGTTGCAGAAGCCTACATTGAAAAG AGGAATGCAACAGAGAGGTACATGCCACGGTATGGACTTCAAAGGAACTTGACCGATTAT E A Y I E K R N A T E P T F R Q
I
M
S
H F S
E V A
R Y M
660
P R Y G E
L Q R N
L
T
D
Y
720
GGTTTGGCCAGGTATGCTTTTGACTTCTACAAACTGACTTCAAGAACTCCTGTGCGTGCT AGAGAAGCACACATGCAAATGAAGGCGGCAGCAGTTAGAGGCAAGTCAACTAGATTATTT G
L A
R Y A
F D
F Y K L T S E
R T
P
V
R
A
780
R
E A H M Q M K A A A V R G K S A
T
R
L
F 840
GGACTTGATGGCAATGTTGGAACAGACGAGGAGAACACAGAGAGACACACAGCAGGAGAT GTCAATCGTGATATGCACACCATGCTTGGTGTTCGTATTTAGAGTATccGTcTTTAAATT G
L D G N V G T D E
E N T E R H
T
A
G
D V N R D M H T M L G V 900
R
I
960
CTCTATAATTTGGCGTTACATTACTTAATACTATGTATTAGTGAGGTTTCACCTCCAGCA TTTTAAATTCAGTATGTGTATCATTCTCTCTACTCTGACAGGGTAAGcAGTTAGTGAGGT
1024 TGCCTCGTGTGAGCCTGATCTTTGTAGAGCGAG (A)3.
Fig. 1. Nucleotide sequence and deduced amino acid sequence of the BYMV coat protein gene. Amino acids differing in the coat protein sequence of the American BYMV isolate
*
are
shown below. The translation stop codon is underlined.
To whom correspondence should be addressed
