Nucleic Acids Research, Vol. 18, No. 24 7445
Nucleotide sequence of protein gene
a
Neurospora
crassa
ribosomal
K.A.Tarawneh, Z.Wang and S.J.Free* Department of Biological Sciences, State University of New York at Buffalo, Buffalo, NY 14260, USA Submitted October 24, 1990 We report the isolation and sequencing of a cDNA encoding a Neurospora crassa ribosomal protein. The cDNA was identified in a differential screening of a Neurospora cDNA library (3). The cDNA was 682 nucleotides in length and contained the translation start and stop codons. The cDNA was found to encode an arginine and lysine-rich protein of 12,935 molecular weight (119 amino acids). Computer analysis of the deduced amino acid sequence indicated that the cDNA encoded a protein which shows 70% amino acid identity with the rat ribosomal protein S26 (2) and 60% amino acid identity to a cloned Drosophila ribosomal protein (1). Characterization of the ribosomal protein mRNA levels indicated that the mRNA levels are regulated in concert with the availability of a utilizable carbon source.
REFERENCES 1. Itoh,N., Ohta,M., Kawasaki,T. and Yamashina,I. (1989) Nucl. Acids Res. 17, 441. 2. Kuwano,Y., Nakanishi,O., Nabeshima,Y.-I., Tanaka,T. and Ogata,K. (1985) J. Biochem. 97, 983-992. 3. McNally,M.T. and Free,S.J. (1988) Curr. Genet. 9, 273-277.
*
To whom correspondence should be addressed
EMBL accession no. X55637 M V K K R K AACAACGGCCGCAACAAGAAGGGCCGCGGCCACGTCAAGCCCATCCGCTGCTCCAACTGCTCG N N G R N K K G R G H V K P I R C S N C S
CGATGCACTCCCAAGGACAAGGCCATCAAGCGCTTCACCATCCGCAACATGGTTGAGTCCGCT R C T P K D K A I K R F T I R N M V E S A GCCATCCGTGATATCTCGGATGCCTCGGTGTTCGCTGAGTACACGGTTCCCAAGATGTACCTC A I R D I S D A S V F A E Y T V P K M Y L AAGCTGCAGTACTGCGTCTCTTGCGCCATTCACGGCAAGATTGTCCGTGTCCGTTCCCGCGAG K L Q Y C V S C A I H G K I V R V R S R E GCCCGCCGCAACCGTGCTCCTCCCCCGCGTGTCCGCTACAACAAGGACGGCAAGAAGGTCACC A R R N R A P P P R V R Y N K D G K K V T CCTACCCAGGGTGCTAAGACCGCCTAAATCAGGCGACCTTAGCGGCTTCCTTGCGACAAACCAG P T Q G A K T A CTTCGGGGTTAACAGGCGTTTTCTGCGTTGGTCGGCGTTTCGATTTTCTGGGGGTTTATATGGA ATGAACATGAGGCCGAAACGAGATGATATCATGACATGGCGCTTTTTTGCACAGAAGCAAATAA GCTGGCAATCACCGGATACCCAAACGGAATCGGAATGGGCATGGGTTCCTTTCGACGAGTTAAG GGATACCAAGCTAATAGTCTACCAATAATCGACGACCTTGAGTACACAAAAAAA....
Figure 1. Nucleotide sequence of a cDNA encoding a Neurospora ribosomal protein and the deduced amino acid sequence.
Nucleotide sequence of protein gene
a
Neurospora
crassa
ribosomal
K.A.Tarawneh, Z.Wang and S.J.Free* Department of Biological Sciences, State University of New York at Buffalo, Buffalo, NY 14260, USA Submitted October 24, 1990 We report the isolation and sequencing of a cDNA encoding a Neurospora crassa ribosomal protein. The cDNA was identified in a differential screening of a Neurospora cDNA library (3). The cDNA was 682 nucleotides in length and contained the translation start and stop codons. The cDNA was found to encode an arginine and lysine-rich protein of 12,935 molecular weight (119 amino acids). Computer analysis of the deduced amino acid sequence indicated that the cDNA encoded a protein which shows 70% amino acid identity with the rat ribosomal protein S26 (2) and 60% amino acid identity to a cloned Drosophila ribosomal protein (1). Characterization of the ribosomal protein mRNA levels indicated that the mRNA levels are regulated in concert with the availability of a utilizable carbon source.
REFERENCES 1. Itoh,N., Ohta,M., Kawasaki,T. and Yamashina,I. (1989) Nucl. Acids Res. 17, 441. 2. Kuwano,Y., Nakanishi,O., Nabeshima,Y.-I., Tanaka,T. and Ogata,K. (1985) J. Biochem. 97, 983-992. 3. McNally,M.T. and Free,S.J. (1988) Curr. Genet. 9, 273-277.
*
To whom correspondence should be addressed
EMBL accession no. X55637 M V K K R K AACAACGGCCGCAACAAGAAGGGCCGCGGCCACGTCAAGCCCATCCGCTGCTCCAACTGCTCG N N G R N K K G R G H V K P I R C S N C S
CGATGCACTCCCAAGGACAAGGCCATCAAGCGCTTCACCATCCGCAACATGGTTGAGTCCGCT R C T P K D K A I K R F T I R N M V E S A GCCATCCGTGATATCTCGGATGCCTCGGTGTTCGCTGAGTACACGGTTCCCAAGATGTACCTC A I R D I S D A S V F A E Y T V P K M Y L AAGCTGCAGTACTGCGTCTCTTGCGCCATTCACGGCAAGATTGTCCGTGTCCGTTCCCGCGAG K L Q Y C V S C A I H G K I V R V R S R E GCCCGCCGCAACCGTGCTCCTCCCCCGCGTGTCCGCTACAACAAGGACGGCAAGAAGGTCACC A R R N R A P P P R V R Y N K D G K K V T CCTACCCAGGGTGCTAAGACCGCCTAAATCAGGCGACCTTAGCGGCTTCCTTGCGACAAACCAG P T Q G A K T A CTTCGGGGTTAACAGGCGTTTTCTGCGTTGGTCGGCGTTTCGATTTTCTGGGGGTTTATATGGA ATGAACATGAGGCCGAAACGAGATGATATCATGACATGGCGCTTTTTTGCACAGAAGCAAATAA GCTGGCAATCACCGGATACCCAAACGGAATCGGAATGGGCATGGGTTCCTTTCGACGAGTTAAG GGATACCAAGCTAATAGTCTACCAATAATCGACGACCTTGAGTACACAAAAAAA....
Figure 1. Nucleotide sequence of a cDNA encoding a Neurospora ribosomal protein and the deduced amino acid sequence.
