0022-5347 /92/14 73-0798$03.00/0 Vol. 147, 798-803, March 1992 Printed in U.S.A.
THE JOURNAL OF UROLOGY
Copyright© 1992 by AMERICAN UROLOGICAL ASSOCIATION, INC.
NUCLEAR LOCALIZATION OF ANDROGEN RECEPTOR IN HETEROGENEOUS SAMPLES OF NORMAL, HYPERPLASTIC AND NEOPLASTIC HUMAN PROSTATE GERALD W. CHODAK,* DAVID M. KRANC, LIBERTAD A. PUY,t HIROYUKI TAKEDA, KATHRYN JOHNSON AND CHAWNSHANG CHANG:j: From the Department of Surgery/Urology and Ben May Institute, University of Chicago, Chicago, Illinois
AI\STRACT
To facilitate an understanding of how androgens participate in the genesis of human benign hyperplasia and carcinoma we assayed androgen receptor in the epithelium and stroma of human prostatic tissue from 57 patients. Immunohistochemical staining of human androgen receptor was performed on 106 sections of normal prostate, benign prostatic hyperplasia (BPH) and prostate cancer. To determine variability of androgen receptor staining sections taken from different portions of the gland were studied. Frozen tissue sections were incubated with monoclonal antiandrogen receptor antibodies and staining was completed by the indirect avidin-biotin peroxidase method. Antibody staining was found mainly in the nucleus of prostatic epithelial cells, although some stromal cells also showed positive staining. Unlike normal prostate, there was a heterogeneous distribution of androgen receptor in BPH and prostate cancer. The androgen receptor content in well differentiated adenocarcinoma epithelium was significantly higher compared to moderately (p 0.05
3
7
2.8 ± 0.5
2.5 ± 0.5
>0.05
No. Pts.
2
Statistical analysis by t test for paired samples. 31 * Corresponds to the total number of cassettes reviewed for each class of tissue studied. t Corresponds to the mean of the sum of the percentage of epithelial cells stained and the staining intensity of those cells. + P values are based on comparison of staining runs.
800
CHODAK AND ASSOCIATES
stromal components a subjective visual estimate on approximately 200 cells was made at X400. The percentage of cells stained was then reported as the number of positive staining cells of the total number counted. Statistical analyses were made by means of modified t test and t test for paired samples.31 RESULTS
Immunohistochemical staining of human androgen receptor was performed on 106 sections from 57 patients (tables 1 and 2). The samples showed normal prostate epithelium in 3 patients (ages 57, 65 and 69 years), BPH in 23 and previously untreated prostate cancer in 31 (ages 49 to 91 years). Immunohistochemical analysis of androgen receptor in normal prostate. The immunolocalization of androgen receptor in normal prostate is shown in figure 1. In all cases androgen receptor was uniformly distributed in the nuclei of epithelial cells. The 3 normal prostates contained a significantly higher mean (plus or minus standard deviation) score of receptor positive cells in the epithelium (percentage of cells stained 2 ± 0, staining intensity 2. 7 ± 0.6, table 1) than in the stroma (percentage of cells stained 1 ± 0, staining intensity 1 ± 0, p
THE JOURNAL OF UROLOGY
Copyright© 1992 by AMERICAN UROLOGICAL ASSOCIATION, INC.
NUCLEAR LOCALIZATION OF ANDROGEN RECEPTOR IN HETEROGENEOUS SAMPLES OF NORMAL, HYPERPLASTIC AND NEOPLASTIC HUMAN PROSTATE GERALD W. CHODAK,* DAVID M. KRANC, LIBERTAD A. PUY,t HIROYUKI TAKEDA, KATHRYN JOHNSON AND CHAWNSHANG CHANG:j: From the Department of Surgery/Urology and Ben May Institute, University of Chicago, Chicago, Illinois
AI\STRACT
To facilitate an understanding of how androgens participate in the genesis of human benign hyperplasia and carcinoma we assayed androgen receptor in the epithelium and stroma of human prostatic tissue from 57 patients. Immunohistochemical staining of human androgen receptor was performed on 106 sections of normal prostate, benign prostatic hyperplasia (BPH) and prostate cancer. To determine variability of androgen receptor staining sections taken from different portions of the gland were studied. Frozen tissue sections were incubated with monoclonal antiandrogen receptor antibodies and staining was completed by the indirect avidin-biotin peroxidase method. Antibody staining was found mainly in the nucleus of prostatic epithelial cells, although some stromal cells also showed positive staining. Unlike normal prostate, there was a heterogeneous distribution of androgen receptor in BPH and prostate cancer. The androgen receptor content in well differentiated adenocarcinoma epithelium was significantly higher compared to moderately (p 0.05
3
7
2.8 ± 0.5
2.5 ± 0.5
>0.05
No. Pts.
2
Statistical analysis by t test for paired samples. 31 * Corresponds to the total number of cassettes reviewed for each class of tissue studied. t Corresponds to the mean of the sum of the percentage of epithelial cells stained and the staining intensity of those cells. + P values are based on comparison of staining runs.
800
CHODAK AND ASSOCIATES
stromal components a subjective visual estimate on approximately 200 cells was made at X400. The percentage of cells stained was then reported as the number of positive staining cells of the total number counted. Statistical analyses were made by means of modified t test and t test for paired samples.31 RESULTS
Immunohistochemical staining of human androgen receptor was performed on 106 sections from 57 patients (tables 1 and 2). The samples showed normal prostate epithelium in 3 patients (ages 57, 65 and 69 years), BPH in 23 and previously untreated prostate cancer in 31 (ages 49 to 91 years). Immunohistochemical analysis of androgen receptor in normal prostate. The immunolocalization of androgen receptor in normal prostate is shown in figure 1. In all cases androgen receptor was uniformly distributed in the nuclei of epithelial cells. The 3 normal prostates contained a significantly higher mean (plus or minus standard deviation) score of receptor positive cells in the epithelium (percentage of cells stained 2 ± 0, staining intensity 2. 7 ± 0.6, table 1) than in the stroma (percentage of cells stained 1 ± 0, staining intensity 1 ± 0, p
