J. Neurovirol. DOI 10.1007/s13365-015-0349-8
NTPDase and 5′-nucleotidase activities in synaptosomes of rabbits experimentally infected with BoHV-5 Cássia B. da Silva 1 & Francine C. Paim 1 & Patricia Wolkmer 2 & Fátima H. Abdalla 3 & Fabiano B. Carvalho 3 & Heloísa H. Palma 2 & Camila B.E. Mello 1 & Eduardo F. Flores 4 & Cinthia M. Andrade 1 & Sonia T.A. Lopes 1
Received: 11 November 2014 / Revised: 21 April 2015 / Accepted: 28 April 2015 # Journal of NeuroVirology, Inc. 2015
Abstract Bovine herpesvirus type 5 (BoHV-5) is the causative agent of herpetic meningoencephalitis in cattle. The purinergic system is described as a modulator of the immune response and neuroinflammation. These functions are related to the extracellular nucleotides concentration. NTPDase and 5′-nucleotidase are enzymes responsible for controlling the extracellular concentration of adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP), and adenosine (ADO). The aim of this study is to determinate the ectonucleotidase activity in cortical synaptosomes and synaptosomes from the hippocampus of rabbits experimentally infected with BoHV-5. Rabbits were divided into four groups, two control groups (non-inoculated animals), and two infected groups (inoculated with BoHV-5). The infected groups received 0.5 ml of BoHV-5 suspension with 107.5TCID50 of viral strain SV-507/99, per paranasal sinuses, * Cássia B. da Silva [email protected] 1
Laboratório de Análises Clínicas Veterinárias e Laboratório de Bioquímica e Estresse Oxidativo, Departamento de Clínica de Pequenos Animais, Universidade Federal de Santa Maria (UFSM), Avenida Roraima 1000, Campus Universitário, Hospital Veterinário, Sala 103, 97105-900 Santa Maria, RS, Brazil
2
Laboratório de Bioquímica e Patologia Clínica Veterinária, Universidade de Cruz Alta, Rodovia Municipal Jacob Della Méa Km 5.6, 98020-290 Cruz Alta, RS, Brazil
3
Laboratório de Bioquímica Toxicológica e Laboratório de Bioquímica e Estresse Oxidativo, Universidade Federal de Santa Maria (UFSM), Avenida Roraima 1000, Campus Universitário, 97105-900 Santa Maria, RS, Brazil
4
Setor de Virologia, Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria (UFSM), Avenida Roraima 1000, Campus Universitário, Prédio 20, 97105-900 Santa Maria, RS, Brazil
and the control groups received 0.5 ml of minimum essential media per paranasal sinuses. Animals were submitted to euthanasia on days 7 and 12 post-inoculation (p.i.); cerebral cortex and hippocampus were collected for the synaptosomes isolation and posterior determination of the ectonucleotidase activities. The results showed a decrease (P < 0.05) in ectonucleotidase activity in synaptosomes from the cerebral cortex of infected rabbits, whereas an increased (P
NTPDase and 5′-nucleotidase activities in synaptosomes of rabbits experimentally infected with BoHV-5 Cássia B. da Silva 1 & Francine C. Paim 1 & Patricia Wolkmer 2 & Fátima H. Abdalla 3 & Fabiano B. Carvalho 3 & Heloísa H. Palma 2 & Camila B.E. Mello 1 & Eduardo F. Flores 4 & Cinthia M. Andrade 1 & Sonia T.A. Lopes 1
Received: 11 November 2014 / Revised: 21 April 2015 / Accepted: 28 April 2015 # Journal of NeuroVirology, Inc. 2015
Abstract Bovine herpesvirus type 5 (BoHV-5) is the causative agent of herpetic meningoencephalitis in cattle. The purinergic system is described as a modulator of the immune response and neuroinflammation. These functions are related to the extracellular nucleotides concentration. NTPDase and 5′-nucleotidase are enzymes responsible for controlling the extracellular concentration of adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP), and adenosine (ADO). The aim of this study is to determinate the ectonucleotidase activity in cortical synaptosomes and synaptosomes from the hippocampus of rabbits experimentally infected with BoHV-5. Rabbits were divided into four groups, two control groups (non-inoculated animals), and two infected groups (inoculated with BoHV-5). The infected groups received 0.5 ml of BoHV-5 suspension with 107.5TCID50 of viral strain SV-507/99, per paranasal sinuses, * Cássia B. da Silva [email protected] 1
Laboratório de Análises Clínicas Veterinárias e Laboratório de Bioquímica e Estresse Oxidativo, Departamento de Clínica de Pequenos Animais, Universidade Federal de Santa Maria (UFSM), Avenida Roraima 1000, Campus Universitário, Hospital Veterinário, Sala 103, 97105-900 Santa Maria, RS, Brazil
2
Laboratório de Bioquímica e Patologia Clínica Veterinária, Universidade de Cruz Alta, Rodovia Municipal Jacob Della Méa Km 5.6, 98020-290 Cruz Alta, RS, Brazil
3
Laboratório de Bioquímica Toxicológica e Laboratório de Bioquímica e Estresse Oxidativo, Universidade Federal de Santa Maria (UFSM), Avenida Roraima 1000, Campus Universitário, 97105-900 Santa Maria, RS, Brazil
4
Setor de Virologia, Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria (UFSM), Avenida Roraima 1000, Campus Universitário, Prédio 20, 97105-900 Santa Maria, RS, Brazil
and the control groups received 0.5 ml of minimum essential media per paranasal sinuses. Animals were submitted to euthanasia on days 7 and 12 post-inoculation (p.i.); cerebral cortex and hippocampus were collected for the synaptosomes isolation and posterior determination of the ectonucleotidase activities. The results showed a decrease (P < 0.05) in ectonucleotidase activity in synaptosomes from the cerebral cortex of infected rabbits, whereas an increased (P
