Diabetologia (2014) 57:204–213 DOI 10.1007/s00125-013-3093-8

ARTICLE

NRF2 plays a protective role in diabetic retinopathy in mice Zhenhua Xu & Yanhong Wei & Junsong Gong & Hongkwan Cho & James K. Park & Ee-Rah Sung & Hu Huang & Lijuan Wu & Charles Eberhart & James T. Handa & Yunpeng Du & Timothy S. Kern & Rajesh Thimmulappa & Alistair J. Barber & Shyam Biswal & Elia J. Duh

Received: 15 August 2013 / Accepted: 7 October 2013 / Published online: 3 November 2013 # Springer-Verlag Berlin Heidelberg 2013

Abstract Aims/hypothesis Although much is known about the pathophysiological processes contributing to diabetic retinopathy (DR), the role of protective pathways has received less attention. The transcription factor nuclear factor erythroid-2-related factor 2 (also known as NFE2L2 or NRF2) is an important regulator of oxidative stress and also has anti-inflammatory effects. The objective of this study was to explore the potential role of NRF2 as a protective mechanism in DR. Methods Retinal expression of NRF2 was investigated in human donor and mouse eyes by immunohistochemistry. The effect of NRF2 modulation on oxidative stress was studied in the human Müller cell line MIO-M1. Non-diabetic and streptozotocin-induced diabetic wild-type and Nrf2 knockout mice were evaluated for multiple DR endpoints. Electronic supplementary material The online version of this article (doi:10.1007/s00125-013-3093-8) contains peer-reviewed but unedited supplementary material, which is available to authorised users. Z. Xu : Y. Wei : J. Gong : H. Cho : J. K. Park : H. Huang : L. Wu : C. Eberhart : J. T. Handa : E. J. Duh (*) Department of Ophthalmology, Johns Hopkins University School of Medicine, 400 N. Broadway, Baltimore, MD 21287, USA e-mail: [email protected] E.

NRF2 plays a protective role in diabetic retinopathy in mice.

Although much is known about the pathophysiological processes contributing to diabetic retinopathy (DR), the role of protective pathways has received ...
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