Mutation Research 763–764 (2014) 28–38
Contents lists available at ScienceDirect
Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis journal homepage: www.elsevier.com/locate/molmut Community address: www.elsevier.com/locate/mutres
Nonhomologous DNA end joining and chromosome aberrations in human embryonic lung fibroblasts treated with environmental pollutants Pavel Rossner Jr. ∗ , Andrea Rossnerova, Olena Beskid, Nana Tabashidze, Helena Libalova, Katerina Uhlirova, Jan Topinka, Radim J. Sram Department of Genetic Ecotoxicology, Institute of Experimental Medicine AS CR, Prague, Czech Republic
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Article history: Received 18 January 2014 Received in revised form 25 February 2014 Accepted 7 March 2014 Available online 30 March 2014 Keywords: Benzo[a]pyrene Chromosome aberrations Double-strand DNA breaks Extractable organic matter Nonhomologous DNA end joining repair
a b s t r a c t In order to evaluate the ability of a representative polycyclic aromatic hydrocarbon (PAH) and PAHcontaining complex mixtures to induce double strand DNA breaks (DSBs) and repair of damaged DNA in human embryonic lung fibroblasts (HEL12469 cells), we investigated the effect of benzo[a]pyrene (B[a]P) and extractable organic matter (EOM) from ambient air particles
Contents lists available at ScienceDirect
Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis journal homepage: www.elsevier.com/locate/molmut Community address: www.elsevier.com/locate/mutres
Nonhomologous DNA end joining and chromosome aberrations in human embryonic lung fibroblasts treated with environmental pollutants Pavel Rossner Jr. ∗ , Andrea Rossnerova, Olena Beskid, Nana Tabashidze, Helena Libalova, Katerina Uhlirova, Jan Topinka, Radim J. Sram Department of Genetic Ecotoxicology, Institute of Experimental Medicine AS CR, Prague, Czech Republic
a r t i c l e
i n f o
Article history: Received 18 January 2014 Received in revised form 25 February 2014 Accepted 7 March 2014 Available online 30 March 2014 Keywords: Benzo[a]pyrene Chromosome aberrations Double-strand DNA breaks Extractable organic matter Nonhomologous DNA end joining repair
a b s t r a c t In order to evaluate the ability of a representative polycyclic aromatic hydrocarbon (PAH) and PAHcontaining complex mixtures to induce double strand DNA breaks (DSBs) and repair of damaged DNA in human embryonic lung fibroblasts (HEL12469 cells), we investigated the effect of benzo[a]pyrene (B[a]P) and extractable organic matter (EOM) from ambient air particles
