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Mutation Research, 48 (1977) 287--294 © Elsevier/North-Holland Biomedical Press
MUTAGEN SCREENING BY A SIMPLIFIED BACTERIAL FLUCTUATION TEST: USE OF MICROSOMAL PREPARATIONS AND WHOLE LIVER CELLS FOR METABOLIC ACTIVATION
M.H.L. GREEN, B.A. BRIDGES, A.M. ROGERS, G. HORSPOOL, W.J. MURIEL, J.W. BRIDGES 1 and J.R. F R Y 1
M.R.C. Cell Mutation Unit, University of Sussex, Falmer, Brighton BN1 9QG and I Department of Biochemistry, University of Surrey, Guildford, Surrey GU2 5XH (Great Britain) (Received November 16th, 1976) (Accepted February 3rd, 1977)
Summary We describe the use of a simplified bacterial fluctuation test to detect induced mutation, either incorporating liver microsomal or whole liver cell preparations. We have evaluated both types of test using three agents. The fluctuation assay seems somewhat slower, simpler and more sensitive than a conventional plating test with microsomes. A whole cell preparation appears marginally more effective than a microsomal fraction for metabolic activation of 9,10
Mutation Research, 48 (1977) 287--294 © Elsevier/North-Holland Biomedical Press
MUTAGEN SCREENING BY A SIMPLIFIED BACTERIAL FLUCTUATION TEST: USE OF MICROSOMAL PREPARATIONS AND WHOLE LIVER CELLS FOR METABOLIC ACTIVATION
M.H.L. GREEN, B.A. BRIDGES, A.M. ROGERS, G. HORSPOOL, W.J. MURIEL, J.W. BRIDGES 1 and J.R. F R Y 1
M.R.C. Cell Mutation Unit, University of Sussex, Falmer, Brighton BN1 9QG and I Department of Biochemistry, University of Surrey, Guildford, Surrey GU2 5XH (Great Britain) (Received November 16th, 1976) (Accepted February 3rd, 1977)
Summary We describe the use of a simplified bacterial fluctuation test to detect induced mutation, either incorporating liver microsomal or whole liver cell preparations. We have evaluated both types of test using three agents. The fluctuation assay seems somewhat slower, simpler and more sensitive than a conventional plating test with microsomes. A whole cell preparation appears marginally more effective than a microsomal fraction for metabolic activation of 9,10
