2805
J. Sep. Sci. 2014, 37, 2805–2813
Raquel Sancho1,2 Arnau Novell1,2 Frantisek Svec3 ´ 1,2 Cristina Minguillon 1 Food
and Nutrition Torribera Campus, University of Barcelona, Santa Coloma de Gramenet, Barcelona, Spain 2 Laboratori de Qu´ımica ` Farmaceutica, Faculty of Pharmacy, University of Barcelona, Barcelona, Spain 3 Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA, USA Received June 11, 2014 Revised July 25, 2014 Accepted July 25, 2014
Research Article
Monolithic silica columns functionalized with substituted polyproline-derived chiral selectors as chiral stationary phases for high-performance liquid chromatography In this study, two polyproline-derived chiral selectors are bonded to monolithic silica gel columns. In spite of high chiral selector coverage, the derivatization was found to have only a slight effect on the hydrodynamics of the mobile phase through the column. The enantioseparation ability of the resulting chiral monolithic columns was evaluated with a series of structurally diverse racemic test compounds. When compared to analogous beadbased chiral stationary phases, higher enantioseparation and broader application domain were observed for monolithic columns. Moreover, the increase in flow rate produces a minor reduction of resolution, which permits to shorten analysis time. Additionally, increased loadability defines chiral polyproline derived monoliths as adequate for preparative chromatography. Keywords: Chiral selectors / Chiral stationary phases / Enantioseparation / PolyL-proline oligomers / Silica monoliths DOI 10.1002/jssc.201400640
1 Introduction As a result of the increasing demand for enantiomerically pure compounds, the chromatographic tools that permit the analysis and production of single enantiomers occupy a relevant position among the most striking developments in separation science. Monolithic materials, either polymeric or based on silica gel, have rapidly become a popular media in diverse separation areas, such as HPLC and CEC [1–4]. Monolithic beds provide a number of advantages in LC because of the flow characteristics that they offer. Backpressures can be maintained
J. Sep. Sci. 2014, 37, 2805–2813
Raquel Sancho1,2 Arnau Novell1,2 Frantisek Svec3 ´ 1,2 Cristina Minguillon 1 Food
and Nutrition Torribera Campus, University of Barcelona, Santa Coloma de Gramenet, Barcelona, Spain 2 Laboratori de Qu´ımica ` Farmaceutica, Faculty of Pharmacy, University of Barcelona, Barcelona, Spain 3 Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA, USA Received June 11, 2014 Revised July 25, 2014 Accepted July 25, 2014
Research Article
Monolithic silica columns functionalized with substituted polyproline-derived chiral selectors as chiral stationary phases for high-performance liquid chromatography In this study, two polyproline-derived chiral selectors are bonded to monolithic silica gel columns. In spite of high chiral selector coverage, the derivatization was found to have only a slight effect on the hydrodynamics of the mobile phase through the column. The enantioseparation ability of the resulting chiral monolithic columns was evaluated with a series of structurally diverse racemic test compounds. When compared to analogous beadbased chiral stationary phases, higher enantioseparation and broader application domain were observed for monolithic columns. Moreover, the increase in flow rate produces a minor reduction of resolution, which permits to shorten analysis time. Additionally, increased loadability defines chiral polyproline derived monoliths as adequate for preparative chromatography. Keywords: Chiral selectors / Chiral stationary phases / Enantioseparation / PolyL-proline oligomers / Silica monoliths DOI 10.1002/jssc.201400640
1 Introduction As a result of the increasing demand for enantiomerically pure compounds, the chromatographic tools that permit the analysis and production of single enantiomers occupy a relevant position among the most striking developments in separation science. Monolithic materials, either polymeric or based on silica gel, have rapidly become a popular media in diverse separation areas, such as HPLC and CEC [1–4]. Monolithic beds provide a number of advantages in LC because of the flow characteristics that they offer. Backpressures can be maintained
