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Monitoring the ionic content of exhaled breath condensate in various respiratory diseases by capillary electrophoresis with contactless conductivity detection
This content has been downloaded from IOPscience. Please scroll down to see the full text. 2015 J. Breath Res. 9 027107 (http://iopscience.iop.org/1752-7163/9/2/027107) View the table of contents for this issue, or go to the journal homepage for more
Download details: IP Address: 131.170.6.51 This content was downloaded on 07/07/2015 at 08:26
Please note that terms and conditions apply.
J. Breath Res. 9 (2015) 027107
doi:10.1088/1752-7155/9/2/027107
paper
received
6 December 2014 re vised
20 February 2015
Monitoring the ionic content of exhaled breath condensate in various respiratory diseases by capillary electrophoresis with contactless conductivity detection
accep ted for publication
2 March 2015 published
6 May 2015
Michal Greguš1,2, František Foret1, Dagmar Kindlová3, Eva Pokojová3, Marek Plutinský3, Martina Doubková3, Zdeněk Merta3, Ilona Binková3, Jana Skřičková3 and Petr Kubáň1 1
Bioanalytical Instrumentation, CEITEC Masaryk University, Veveri 97, 602 00, Brno, Czech Republic Department of Chemistry, Masaryk University, Kotlarska 2, 611 37 Brno, Czech Republic 3 Department of Respiratory Diseases and TB, University Hospital Brno and Faculty of Medicine, Masaryk University Brno, 601 77 Brno, Czech Republic 2
E-mail: [email protected] Keywords: exhaled breath condensate, capillary electrophoresis, contactless conductivity detection, anions and cations, respiratory diseases
Abstract The analysis of an ionic profile of exhaled breath condensate (EBC) by capillary electrophoresis with contactless conductivity detection and double opposite end injection, is demonstrated. A miniature sampler made from a 2 ml syringe and an aluminium cooling cylinder was used for the fast collection of EBC (under one minute). Analysis of the collected EBC was performed in a 60 mM 2-(N-morpholino)ethanesulfonic acid, 60 mM L-histidine background electrolyte with 30 µM cetyltrimethylammonium bromide and 2 mM 18-crown-6 at pH 6, and excellent repeatability of migration times (RSD
Search
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My IOPscience
Monitoring the ionic content of exhaled breath condensate in various respiratory diseases by capillary electrophoresis with contactless conductivity detection
This content has been downloaded from IOPscience. Please scroll down to see the full text. 2015 J. Breath Res. 9 027107 (http://iopscience.iop.org/1752-7163/9/2/027107) View the table of contents for this issue, or go to the journal homepage for more
Download details: IP Address: 131.170.6.51 This content was downloaded on 07/07/2015 at 08:26
Please note that terms and conditions apply.
J. Breath Res. 9 (2015) 027107
doi:10.1088/1752-7155/9/2/027107
paper
received
6 December 2014 re vised
20 February 2015
Monitoring the ionic content of exhaled breath condensate in various respiratory diseases by capillary electrophoresis with contactless conductivity detection
accep ted for publication
2 March 2015 published
6 May 2015
Michal Greguš1,2, František Foret1, Dagmar Kindlová3, Eva Pokojová3, Marek Plutinský3, Martina Doubková3, Zdeněk Merta3, Ilona Binková3, Jana Skřičková3 and Petr Kubáň1 1
Bioanalytical Instrumentation, CEITEC Masaryk University, Veveri 97, 602 00, Brno, Czech Republic Department of Chemistry, Masaryk University, Kotlarska 2, 611 37 Brno, Czech Republic 3 Department of Respiratory Diseases and TB, University Hospital Brno and Faculty of Medicine, Masaryk University Brno, 601 77 Brno, Czech Republic 2
E-mail: [email protected] Keywords: exhaled breath condensate, capillary electrophoresis, contactless conductivity detection, anions and cations, respiratory diseases
Abstract The analysis of an ionic profile of exhaled breath condensate (EBC) by capillary electrophoresis with contactless conductivity detection and double opposite end injection, is demonstrated. A miniature sampler made from a 2 ml syringe and an aluminium cooling cylinder was used for the fast collection of EBC (under one minute). Analysis of the collected EBC was performed in a 60 mM 2-(N-morpholino)ethanesulfonic acid, 60 mM L-histidine background electrolyte with 30 µM cetyltrimethylammonium bromide and 2 mM 18-crown-6 at pH 6, and excellent repeatability of migration times (RSD
