Appl Microbiol Biotechnol (2014) 98:23–29 DOI 10.1007/s00253-013-5370-3
MINI-REVIEW
Molecular engineering of industrial enzymes: recent advances and future prospects Haiquan Yang & Jianghua Li & Hyun-dong Shin & Guocheng Du & Long Liu & Jian Chen
Received: 18 September 2013 / Revised: 28 October 2013 / Accepted: 30 October 2013 / Published online: 19 November 2013 # Springer-Verlag Berlin Heidelberg 2013
Abstract Many enzymes are efficiently produced by microbes. However, the use of natural enzymes as biocatalysts has limitations such as low catalytic efficiency, low activity, and low stability, especially under industrial conditions. Many protein engineering technologies have been developed to modify natural enzymes and eliminate these limitations. Commonly used protein engineering strategies include directed evolution, site-directed mutagenesis, truncation, and terminal fusion. This review summarizes recent advances in the molecular engineering of industrial enzymes and discusses future prospects in this field. We expect this review to increase interest in and advance the molecular engineering of industrial enzymes.
Keywords Enzymes . Directed evolution . Site-directed mutagenesis . Saturation mutagenesis . Truncation . Fusion H. Yang : J. Li : G. Du : L. Liu Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China H. Yang : J. Li : G. Du : L. Liu Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China J. Li : G. Du : L. Liu (*) : J. Chen Synergetic Innovation Center of Food Safety and Nutrition, Wuxi 214122, China e-mail: [email protected] H.
MINI-REVIEW
Molecular engineering of industrial enzymes: recent advances and future prospects Haiquan Yang & Jianghua Li & Hyun-dong Shin & Guocheng Du & Long Liu & Jian Chen
Received: 18 September 2013 / Revised: 28 October 2013 / Accepted: 30 October 2013 / Published online: 19 November 2013 # Springer-Verlag Berlin Heidelberg 2013
Abstract Many enzymes are efficiently produced by microbes. However, the use of natural enzymes as biocatalysts has limitations such as low catalytic efficiency, low activity, and low stability, especially under industrial conditions. Many protein engineering technologies have been developed to modify natural enzymes and eliminate these limitations. Commonly used protein engineering strategies include directed evolution, site-directed mutagenesis, truncation, and terminal fusion. This review summarizes recent advances in the molecular engineering of industrial enzymes and discusses future prospects in this field. We expect this review to increase interest in and advance the molecular engineering of industrial enzymes.
Keywords Enzymes . Directed evolution . Site-directed mutagenesis . Saturation mutagenesis . Truncation . Fusion H. Yang : J. Li : G. Du : L. Liu Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China H. Yang : J. Li : G. Du : L. Liu Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China J. Li : G. Du : L. Liu (*) : J. Chen Synergetic Innovation Center of Food Safety and Nutrition, Wuxi 214122, China e-mail: [email protected] H.
