EXPERIMENTAL AND THERAPEUTIC MEDICINE 11: 1803-1808, 2016
miR141 expression is downregulated and negatively correlated with STAT5 expression in esophageal squamous cell carcinoma HONGWU TAN, YUNFENG ZHU, JILING ZHANG, LIJUN PENG and TAO JI Department of Gastroenterology, Linyi People's Hospital, Linyi, Shandong 276000, P.R. China Received October 6, 2014; Accepted October 29, 2015 DOI: 10.3892/etm.2016.3098 Abstract. The aim of the present study was to investigate the association between microRNA‑141 (miR141) and signal transducer and activator of transcription 5 (STAT5) expression levels in human esophageal squamous cell carcinoma (ESCC) and to investigate the effects of miR141 on ESCC cells. A total of 45 consecutive patients with ESCC were enrolled in the study. The expression of miR141 in ESCC tissue samples was detected using reverse transcription quantitative polymerase chain reaction (RT‑qPCR). The expression of STAT5 in the ESCC tissues was detected using immunohistochemical staining and western blotting. In addition, Eca109 cells were transfected with miR141 mimic, and the levels of STAT5 were detected using western blotting. The effects of miR141 on the proliferation, invasion and migration of the cells were also detected using MTT, scratch and Transwell invasion assays, respectively. The miR141 expression level in the ESCC tissue samples was significantly decreased compared with that in the adjacent normal tissues (P
miR141 expression is downregulated and negatively correlated with STAT5 expression in esophageal squamous cell carcinoma HONGWU TAN, YUNFENG ZHU, JILING ZHANG, LIJUN PENG and TAO JI Department of Gastroenterology, Linyi People's Hospital, Linyi, Shandong 276000, P.R. China Received October 6, 2014; Accepted October 29, 2015 DOI: 10.3892/etm.2016.3098 Abstract. The aim of the present study was to investigate the association between microRNA‑141 (miR141) and signal transducer and activator of transcription 5 (STAT5) expression levels in human esophageal squamous cell carcinoma (ESCC) and to investigate the effects of miR141 on ESCC cells. A total of 45 consecutive patients with ESCC were enrolled in the study. The expression of miR141 in ESCC tissue samples was detected using reverse transcription quantitative polymerase chain reaction (RT‑qPCR). The expression of STAT5 in the ESCC tissues was detected using immunohistochemical staining and western blotting. In addition, Eca109 cells were transfected with miR141 mimic, and the levels of STAT5 were detected using western blotting. The effects of miR141 on the proliferation, invasion and migration of the cells were also detected using MTT, scratch and Transwell invasion assays, respectively. The miR141 expression level in the ESCC tissue samples was significantly decreased compared with that in the adjacent normal tissues (P
