Nucleic Acids Research, Vol. 20, No. 16 4371
Minimizing deletion mutagenesis artifact during Taq DNA polymerase PCR by E.coli SSB Quin Chou* Cetus Corporation, 1400 Fifty-third street, Emeryville, CA 94608, USA Submitted May 28, 1992 Many artifacts during Taq DNA polymerase PCR have been reported, especially with PCR targets containing stable secondary structure (1, 2, 3, 4). The consequence with PCR targets containing stable secondary structure usually is either very low efficiency in PCR yield, or deletion mutagenesis in the PCR products (1, 2). Pallansch et al. have estimated that AG for such stable secondary structures calculated by the FOLD computer program is
Minimizing deletion mutagenesis artifact during Taq DNA polymerase PCR by E.coli SSB Quin Chou* Cetus Corporation, 1400 Fifty-third street, Emeryville, CA 94608, USA Submitted May 28, 1992 Many artifacts during Taq DNA polymerase PCR have been reported, especially with PCR targets containing stable secondary structure (1, 2, 3, 4). The consequence with PCR targets containing stable secondary structure usually is either very low efficiency in PCR yield, or deletion mutagenesis in the PCR products (1, 2). Pallansch et al. have estimated that AG for such stable secondary structures calculated by the FOLD computer program is
