EXPERIMENTAL AND THERAPEUTIC MEDICINE 9: 961-966, 2015
microRNA-184 functions as tumor suppressor in renal cell carcinoma ZHENGMING SU1-3, DUQUN CHEN1,3,4, YIFAN LI1,4, ENPU ZHANG1,3, ZUHU YU1,4, TING CHEN1,2, ZHIMAO JIANG1,3, LIANGCHAO NI1,3, SHANGQI YANG1,3, YAOTING GUI1,3, JIONGXIAN YE1-3 and YONGQING LAI1-3 1
Department of Urology, Peking University Shenzhen Hospital, Institute of Urology of Shanghai PKU‑HKUST Medical Center, Shenzhen, Guangdong 518036; 2Shantou University Medical College, Shantou, Guangdong 515041; 3 Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen PKU-HKUST Medical Center, Shenzhen, Guangdong 518036; 4Anhui Medical University, Hefei, Anhui 230032, P.R. China Received April 10, 2014; Accepted January 12, 2015 DOI: 10.3892/etm.2015.2199 Abstract. microRNAs (miRNAs) are evolutionarily conserved, endogenous, small, noncoding RNA molecules of approximately 22 nucleotides in length that function as post‑transcriptional gene regulators. Their aberrant expression may be involved in human diseases, including cancer. Although miRNA‑184 (miR‑184) has been reported in other tumors, its function in renal cell carcinoma (RCC) is still unknown. The aim of the present study was to investigate the role of miR‑184 in RCC. The impacts of miR‑184 on cell migration, proliferation and apoptosis were evaluated using migration scratch, 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide (MTT) and flow cytometry assay. Our studies revealed that miR‑184 mimic significantly inhibits cell migration, suppresses cell proliferation and induces renal cancer cell apoptosis in vitro when compared with the negative control (P
microRNA-184 functions as tumor suppressor in renal cell carcinoma ZHENGMING SU1-3, DUQUN CHEN1,3,4, YIFAN LI1,4, ENPU ZHANG1,3, ZUHU YU1,4, TING CHEN1,2, ZHIMAO JIANG1,3, LIANGCHAO NI1,3, SHANGQI YANG1,3, YAOTING GUI1,3, JIONGXIAN YE1-3 and YONGQING LAI1-3 1
Department of Urology, Peking University Shenzhen Hospital, Institute of Urology of Shanghai PKU‑HKUST Medical Center, Shenzhen, Guangdong 518036; 2Shantou University Medical College, Shantou, Guangdong 515041; 3 Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen PKU-HKUST Medical Center, Shenzhen, Guangdong 518036; 4Anhui Medical University, Hefei, Anhui 230032, P.R. China Received April 10, 2014; Accepted January 12, 2015 DOI: 10.3892/etm.2015.2199 Abstract. microRNAs (miRNAs) are evolutionarily conserved, endogenous, small, noncoding RNA molecules of approximately 22 nucleotides in length that function as post‑transcriptional gene regulators. Their aberrant expression may be involved in human diseases, including cancer. Although miRNA‑184 (miR‑184) has been reported in other tumors, its function in renal cell carcinoma (RCC) is still unknown. The aim of the present study was to investigate the role of miR‑184 in RCC. The impacts of miR‑184 on cell migration, proliferation and apoptosis were evaluated using migration scratch, 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide (MTT) and flow cytometry assay. Our studies revealed that miR‑184 mimic significantly inhibits cell migration, suppresses cell proliferation and induces renal cancer cell apoptosis in vitro when compared with the negative control (P
