Microbiology Papers in Press. Published January 29, 2015 as doi:10.1099/mic.0.000046

Microbiology Microcystin LR induced cytotoxicity and apoptosis in human embryonic kidney (HEK 293) and human kidney adenocarcinoma (ACHN) cell lines --Manuscript Draft-Manuscript Number:

MIC-D-14-00051R1

Full Title:

Microcystin LR induced cytotoxicity and apoptosis in human embryonic kidney (HEK 293) and human kidney adenocarcinoma (ACHN) cell lines

Short Title:

Microcystin LR induced toxicity in human embryonic kidney (HEK 293) and human kidney adenocarcinoma (ACHN) cell lines

Article Type:

Standard

Section/Category:

Environmental and Evolutionary Microbiology

Corresponding Author:

Poorna Piyathilaka University of Sri Jayewardenepura Nugegoda, SRI LANKA

First Author:

Poorna Piyathilaka

Order of Authors:

Poorna Piyathilaka Pathmalal Manage, PhD Kamani Tennekoon Nissanka De Silva Sameera Samarakoon Chanthirika Shanmuganathan

Abstract:

Microcystin-LR (MC-LR) is a potent hepatotoxin, and increasing evidence suggests that it might also induce kidney injury. The aim of present work was to evaluate the cytotoxicity and possible apoptotic effects of MC-LR on human embryonic kidney cell line (HEK-293) and human kidney adenocarcinoma cell line (ACHN). Cells were exposed for 24 h to pure MC-LR (1.0-200 µM) and the cytotoxic effects were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Sulphorhodamine B (SRB) cell viability assays. Cell viability in both cell lines were significantly decreased after treatment with MC-LR at 50 µM for 24 h (p

Microcystin-LR-induced cytotoxicity and apoptosis in human embryonic kidney and human kidney adenocarcinoma cell lines.

Microcystin-LR (MC-LR) is a potent hepatotoxin, and increasing evidence suggests that it might also induce kidney injury. The aim of the present work ...
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