Cancers 2015, 7, 763-783; doi:10.3390/cancers7020763 OPEN ACCESS

cancers ISSN 2072-6694 www.mdpi.com/journal/cancers Review

Mechanisms of Acquired Resistance to ALK Inhibitors and the Rationale for Treating ALK-positive Lung Cancer Hideko Isozaki 1, Nagio Takigawa 2,* and Katsuyuki Kiura 3 1

2 3

Department of Clinical Pharmaceutics, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama 700-8558, Japan; E-Mail: [email protected] Department of General Internal Medicine 4, Kawasaki Medical School, Okayama 700-8505, Japan Department of Allergy and Respiratory Medicine, Okayama University Hospital, Okayama 700-8558, Japan; E-Mail: [email protected]

* Author to whom correspondence should be addressed; E-Mail: [email protected]. Academic Editor: Siow Ming Lee Received: 28 February 2015 / Accepted: 21 April 2015 / Published: 30 April 2015

Abstract: The discovery of an echinoderm microtubule-associated protein-like 4 (EML4)anaplastic lymphoma kinase (ALK) fusion gene led to improved clinical outcomes in patients with lung cancer after the development of the first ALK-targeting agent, crizotinib. Some second-generation ALK tyrosine kinase inhibitors (TKIs), which might be more potent than crizotinib or effective on crizotinib-resistant patients, have been developed. Although these ALK-TKIs show an excellent response initially, most patients eventually acquire resistance. Therefore, careful consideration of the resistance mechanisms might lead to superior therapeutic strategies. Here, we summarize the history of ALK-TKIs and their underlying resistance mechanisms in both the preclinical and clinical settings. In addition, we discuss potential future treatment strategies in ALK-TKI-naï ve and -resistant patients with lung cancer harboring the EML4-ALK fusion gene. Keywords: lung cancer; EML4-ALK; crizotinib; ceritinib; alectinib; resistance

1. Introduction Several gene aberrations are known to cause different types of cancer. This knowledge has led to the development of molecular-targeted drugs, and cancer treatment has progressed impressively over the

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past decade. The first important breakthrough was the development of imatinib, a BCR-ABL tyrosine kinase inhibitor (TKI), for chronic myeloid leukemia (CML) patients harboring the Philadelphia chromosome, which results from a translocation between chromosomes 9 and 22. This drug achieved long-term complete remission, which has excited physicians around the world. After 8 years of follow up, the complete cytogenetic response rate was 83%, and 55% of patients were still receiving imatinib. Only 7% of the patients died from CML, and the overall survival (OS) rate was 85% [1,2]. Subsequently, understanding the correlation between a mutation in the epidermal growth factor receptor (EGFR) gene and the response to gefitinib improved treatment strategies for advanced non-small cell lung cancer (NSCLC) [3,4]. Maemondo et al. reported that treatment with gefitinib achieved a longer median progression-free survival (PFS) of 10.8 months and a higher response rate of 73.7% compared with standard chemotherapy in NSCLC patients with EGFR mutations [5]. Following the success of EGFR TKIs, the discovery of the echinoderm microtubule-associated protein-like 4 (EML4)-anaplastic lymphoma kinase (ALK) fusion gene in 2007 elicited a significant change in the therapeutic strategies for NSCLC patients harboring the specific aberrant gene [6]. ALK is located on chromosome 2, and its gene product plays a role in brain development and acts on specific neurons in the nervous system [7]. An aberration in the ALK gene was reported first in patients with anaplastic large-cell lymphoma and inflammatory myofibroblastic tumors with an ALK translocation and ALK amplification, respectively [8,9]. In addition to the EML4-ALK fusion gene in lung cancer, various ALK-related diseases have been reported, including familial neuroblastoma [10], renal cell carcinomas [11–14], esophageal squamous cell carcinomas [15,16], breast cancer, colonic adenocarcinomas [17], glioblastoma multiforme [18,19], and anaplastic thyroid cancer [20]. These tumors were sensitive to ALK-TKIs [21,22]. Treatment with the first-generation ALK-TKI crizotinib exhibited prominent efficacy and became the standard therapy for NSCLC patients harboring the EML4-ALK fusion gene. As stated above, the molecular-targeted agents identified to date have achieved outstanding efficacy. However, acquired (or initial) drug resistance is an inevitable problem. Herein we review the history of ALK-TKIs and their underlying resistance mechanisms. In addition, we summarize future therapeutic strategies for ALK-positive lung cancer patients. 2. Crizotinib Crizotinib, a small molecule compound, can inhibit multiple tyrosine kinases and was developed initially for targeting MET. The EML4-ALK fusion gene was discovered as a novel driver oncogene in 2007 [6], and crizotinib has received attention, because it can also inhibit the ALK tyrosine kinase. Crizotinib is highly effective in patients with ALK-positive NSCLC, similarly to the effects of imatinib in BCR-ABL-positive CML or of gefitinib in EGFR-mutated NSCLC. It was reported that crizotinib achieved a longer PFS than did standard chemotherapy in advanced or metastatic ALK-positive NSCLC patients when used in both the first- and second-line settings (Table 1). Although molecular-targeted compounds have elicited a good response in tumors expressing the specific target, crizotinib resistance also eventually occurs in almost all patients. The mechanisms of crizotinib resistance in patients and cell lines reported previously are summarized in Table 2 and Figure 1.

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765 Table 1. Clinical Trials.

Drugs Crizotinib

Ceritinib

Trial

Phase

Prior treatment with ALK-TKI

ORR

PFS

OS estimated 6 M: 87.9% 12 M: 74.8%

CNS disease

Reference

PROFILE 1001

1

No

143

60.8%

9.7 M

PROFILE 1005

2

No (chemotherapy: Yes)

439

53%

8.5 M

PROFILE 1007

3

No (platinum-based chemotherapy: Yes)

347 173 vs. 174

65% vs. 20%

7.7 vs. 3.0 M

12.2 vs. 12.1 M

[25]

PROFILE 1014

3

No

343 172 vs. 171

74% vs. 45%

10.9 vs. 7.0 M

probability 12 M: 84% vs. 79%

[26]

ASCEND-1

1

Yes (163/246)

246

58%

8.2 M

12 M: 65%

93.5% CR rate: 19.6%

27.7 M

12 M: 83% 24 M: 79%

55% CR rate: 2%

NA

ORR: 52%

[30]

10.9M

69% improved CNS disease

[31]

AF-001JP

1/2

No

Phase 1: 24 Phase 2: 46

AF-002JG

1/2

Yes

Phase 1: 47

Alectinib

AP26113

No. of patients

Gadgeel et al.

1/2

Yes

57

72%

[23] [24]

ORR: 54%

[27] [28,29]

ORR: objective response rate; PFS: progression free survival; CNS: central nervous system; CR: complete response; M: month; TKI: tyrosine kinase inhibitor.

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Mechanisms ALK amplification

ALK amplification + ALK mutation

CNG

a patient

CNG + G1269A

a patient

CNG + L1196M CNG + 1151Tins

ALK alteration

L1196M, C1156Y

F1174L

S1206Y, G1202R, L1196M

H3122CR1 cells (stepwise increase) H3122CR2 cells (stepwise increase) a patient Ba/F3 cells (transfected mutation) a patient Ba/F3 cells (transfected mutation) patients

Number of patients 1 of 11 patients (12 samples) 1 of 11 patients (12 samples)

Ba/F3 cells (transfected mutation)

L1196M, G1269A

patients

G1202R

a patient H3122 cells (transfected mutation)

L1152R

Reference [32] [32]

TAE684, AP26113, 17-AAG

[33]

17-AAG

[34]

1

[35]

1

[36] TAE684, 17-AAG

3 of 18 patients (19 samples)

[34] S1206Y： TAE684, alectinib, 17-AAG; G1202R： TAE684, 17-AAG; L1196M： TAE684, 17-AAG, alectinib; 1151Tins： TAE684, 17-AAG, alectinib (Agents above showing lower IC50 than crizotinib)

ALK mutation S1206Y, G1202R, L1196M, 1151Tins

Agents for overcoming the resistance

4 of 11 patients (12 samples) 1

[34]

[32] [37] [38]

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767 Table 2. Cont. Mechanisms L1152R

ALK mutation + EGFR activation

secretion of EGFR ligand (amphireglin)

Bypass track activation

1151Tins increased auto-phosphorylation of EGFR L858R retained phosphorylation of EGFR

EGFR activation

secretion of EGFR ligand (amphiregulin) and ErbB3 ligand (NRG1) increased auto-phosphorylation of EGFR

Material (patient or cell line) a patient DFCI076 cells (derived from the above-referenced patient) a patient a patient H3122 cells (external EGF) H2228 cells, H3122 cells (external EGF, TGF-α and HB-EGF)

Number of patients 1

Reference [38]

ALK inhibitor + PF299804

[38]

1 of 18 patients (19 samples)

[34]

1

[32] crizotinib + PF299804 or gefitinib

[38]

[39]

H3122CR3 (stepwise increase)

a patient

Agents for overcoming the resistance

crizotinib + gefitinib or erlotinib

1 of 9 patients

[34]

[34]

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Number of patients

Increased auto-phosphorylation of EGFR KIT amplification + SCF overexperssion

a patient

1 of 9 patients

KIT amplification

a patient

2 of 18 patients (19 samples)

increased phosphorylation of cKIT

H3122 (overexpressed cKIT + external SCF)

Mechanisms

Bypass track activation

EGFR + KIT activation

KIT activation

patients KRAS mutation

IGF-1R activation

G12V

increased phosphorylation of IGF-1R

CUTO-1 cells (derived from above patient; ALK-, KRAS+) a patient H3122 (external IGF-1R)

Agents for overcoming the resistance

Reference

[34]

[34] crizotinib + imatinib 2 of 11 patients (12 samples) (1 of 2 is intrinsic resistances)

[32]

[32] 1

[37] crizotinib + OSI-906

[37]

CNG: Copy number gain; TAE684, alectinib: ALK inhibitor; 17-AAG: HSP90 inhibitor; PF299804: HER1, 2, and 4 inhibitor; OSI-906: IGF1R inhibitor; IC50: inhibitory concentration of 50%.

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Figure 1. Mechanisms of resistance to crizotinib. 2.1. ALK Secondary Mutations The genetic alterations underlying the acquired resistance to molecular-targeted agents such as T315I in BCR-ABL or T790M in EGFR have been reported previously [40–42]. Approximately 50% of NSCLC patients who acquire resistance to gefitinib exhibit secondary mutations. Among the reported mutations, >90% of the resistant tumors have a T790M gatekeeper mutation, which is present in the binding site for competitive inhibitors in the adenosine triphosphate (ATP)-binding pocket of various kinases [43]. Additional mutation sites in EGFR-TKI resistant tumors have been reported only rarely. In contrast, crizotinib-resistant ALK-positive NSCLC tumors have multiple gene alterations both inside and outside the gatekeeper site, similar to imatinib-resistant gene alterations. Katayama et al. performed a comprehensive investigation of re-biopsy samples derived from 18 crizotinib-refractory patients and found secondary ALK mutations (S1206Y, G1202R, L1196M, and 1151Tins) in four patients [34]. Doeble et al. also observed two different mutations (L1196M and G1269A) within the tyrosine kinase domain in four of nine patients with acquired resistance to crizotinib [32]. Based on the two reports described above, various ALK secondary mutations might occur in ~30% of crizotinib-refractory patients (Figure 2). Furthermore, multiple gene alterations were observed in one patient. Choi et al. described two different secondary mutations in the kinase domain of EML4-ALK in tumor cells isolated from a crizotinib-refractory patient [35], one of which was the gatekeeper mutation L1196M, which corresponds to T315I in BCR-ABL and T790M in EGFR. The other mutation was C1156Y, which is located within the ALK kinase domain N-terminal to the αC-helix. These results

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suggest that secondary alterations to ALK in crizotinib-resistant patients can present with heterogeneity, even within a single tumor.

Figure 2. Approximate proportion of crizotinib-resistant mechanisms [32,34]. ALK secondary mutations include 1151Tins, L1152R, C1156Y, F1174L, L1196M, G1202R, S1206Y, and G1269A. Some second generation ALK inhibitors have been developed, including ceritinib and alectinib, which could overcome the ALK secondary mutations [44,45]. Tumors with different mutations exhibit different sensitivities to the agents. Thus, differing approaches according to the type of mutation are required to overcome crizotinib resistance, including ALK secondary mutations. To date, two ALK secondary mutations that seem to be refractory to ceritinib and/or alectinib have been reported: cells with the G1202R mutation were insensitive to ceritinib and alectinib, and cells with the F1174C mutation were insensitive to ceritinib [34,45,46]. The sensitivity of cells with F1174C mutations to alectinib has not been reported. The development of novel agents that are effective in NSCLC patients with the secondary mutations described above is expected in the near future. 2.2. ALK Amplification A gain in ALK gene fusion copy number was implicated as a mechanism of resistance to crizotinib in a cell line model [33]. Cells resistant to an intermediate dose of crizotinib (0.6 μM) developed an amplification in the ALK gene, which was retained in cells resistant to a high dose (1 μM) of crizotinib. Interestingly, cells that were completely resistant also harbored the gatekeeper mutation L1196M, which was not detected in the partially resistant cells. Doeble et al. also reported an ALK copy number gain in two re-biopsied samples [32]. One sample had an ALK secondary mutation (G1269A) together with ALK amplification, and the other had no secondary mutation but possessed an ALK copy number gain of 2.2-fold, which was the highest gain among the 10 samples examined in the study. Thus, ALK secondary mutations might occur when ALK-positive tumors harboring a low level of ALK amplification acquire high crizotinib resistance. In addition, if ALK amplification in the absence of a secondary mutation causes crizotinib resistance, a high level of amplification may result in the resistant tumor. A preclinical

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study demonstrated that a second-generation ALK inhibitor or an HSP90 inhibitor might abrogate this resistance [33,34]. 2.3. Loss of ALK In addition to secondary ALK mutations and ALK amplification, the loss of ALK or other alterations in driver oncogenes were reported in crizotinib-resistant tumors. Two of nine ALK-positive NSCLC tumors lost the EML4-ALK fusion gene after treatment failure with crizotinib [32]. Of these, one sample showed loss of ALK in the first re-biopsy performed 61 days after the initiation of crizotinib treatment. However, the ALK fusion gene was re-detected in a second re-biopsy after 113 days on crizotinib. It is difficult to prove loss of ALK in clinical samples, because ALK detection is affected easily by various factors. In addition, we always have to consider whether the specimens contain sufficient cancer cells for analysis and whether the processes used to obtain formalin-fixed paraffin-embedded sections and store samples are appropriate [47]. A recent report demonstrated that loss of the ALK fusion gene occurred in one crizotinib-refractory NSCLC patient using next generation sequencing [48]. Nevertheless, future investigations using preclinical models and more clinical samples are needed to determine whether loss of ALK is a mechanism of resistance to crizotinib. 2.4. EGFR Activation In addition to ALK gene alterations, other mechanisms of resistance to crizotinib include the activation of tyrosine kinase receptors such as EGFR, KRAS, or cKIT. Because these resistance mechanisms partially retain ALK survival signaling (namely the co-activation of ALK and other tyrosine kinase pathways), blocking both pathways is likely required to overcome this resistance. Sasaki et al. detected the secretion of amphiregulin, which is an EGFR ligand, in DFCI076 cells derived from a crizotinib-refractory patient [38]. DFCI076 cells had no ALK secondary mutations and displayed increased levels of activated EGFR compared with H3122 cells. They also confirmed that the presence of exogenous EGF caused crizotinib resistance in H3122 cells, suggesting that EGFR activation might be induced by increased EGFR ligand levels. Yamada et al. also revealed that paracrine EGFR ligands including EGF, TGF-α, and heparin-binding EGF-like growth factor (HB-EGF) caused crizotinib resistance [39]. Consistent with this phenomenon, Katayama et al. observed increased levels of EGFR mRNA, amphiregulin, and HER3 ligand (NRG1) in the resistant cells, in which activation of EGFR and HER3 was increased compared with the parental cells [34]. Four of nine crizotinib-resistant tumors expressed increased levels of phosphorylated EGFR compared with pre-resistant samples. In addition, a preclinical study showed that the combination of crizotinib and EGFR-TKIs might be effective against this resistance [34,38]. Katayama et al. found that NSCLC with an ALK secondary mutation (1151Tins) expressed activated EGFR [34]. Thus, the activation of alternative survival signaling pathways could coexist with ALK secondary mutations.

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2.5. cKIT Amplification Crizotinib resistance caused by amplification of the cKIT gene was reported in two samples derived from crizotinib-refractory patients [34]. One tumor expressed high levels of the KIT ligand, stem cell factor (SCF) in stromal cells within the solid component. H3122 cells overexpressing KIT were sensitive to crizotinib in the absence of exogenous SCF, although they remained resistant to crizotinib in the presence of SCF. Therefore, this type of resistance might require both activated tyrosine kinase receptor and presence of the ligand. Treatment with imatinib, a small molecule inhibitor of KIT, reversed the resistance phenotype completely, suggesting that imatinib is useful for treating KIT-overexpressing crizotinib-refractory NSCLC. 2.6. KRAS Mutation Oncogenic mutations within the KRAS gene (G12C or G12V) were reported in two of 11 NSCLC patients [32]. However, given that one patient had a short duration to progression despite treatment with crizotinib and harbored a KRAS G12C mutation ab initio, this case was considered to be intrinsic resistance. The other patient exhibited a KRAS G12V mutation; however, introducing a G12V mutation into the KRAS gene did not elicit crizotinib resistance in H3122 cells. Thus, it remains unclear whether the acquisition of a KRAS mutation could serve as a direct mechanism of acquired resistance to crizotinib. 2.7. IGF-1R Activation A previous study reported that expression of IGF-1R and insulin receptor substrate-1 (IRS-1), which is an adaptor protein that binds to IGF-1R or ALK, were related to crizotinib resistance [37]. Three tumor biopsies taken at the time of acquired resistance expressed increased levels of pIGF-1R compared with the respective pre-resistant tumors. Two of three samples had increased IRS-1 expression after crizotinib treatment compared with pretreatment. In one tumor, IGF-1R and IRS-1 mRNA levels were increased after crizotinib treatment. The preclinical study suggested that the combination of crizotinib and an IGF-1R inhibitor might overcome this resistance. They also found that there was no significant change in IRS-1 levels between before and after EGFR-TKI therapy in EGFR-mutant lung tumor. Namely, the changes in IRS-1 might be specific to ALK-positive lung cancer. 3. Ceritinib On April 29, 2014, the Food and Drug Administration (FDA) approved the use of ceritinib for the treatment of ALK-positive metastatic NSCLC patients who are intolerant to crizotinib or have progressed after crizotinib treatment. Ceritinib is a second-generation ALK-TKI that can block not only ALK, but also IGF-1R and INSR [37,49]. In addition, previously untreated patients with central nervous system (CNS) involvement also achieved a favorable response to ceritinib, with an overall response rate (ORR) of 72.3% and a PFS of 18.4 months [50]. In a phase 1 study of patients with advanced NSCLC harboring ALK rearrangements, ceritinib demonstrated good efficacy, with an ORR of 58% and a median PFS of 7.0 months [27]. Subgroup analyses revealed that ceritinib exhibited good activity in crizotinib-refractory patients (ORR, 56%) as well as in patients that had not received crizotinib (ORR, 62%). However, differences in the median PFS were observed among subgroups: the median PFS of

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crizotinib-refractory patients was 6.9 months, whereas the crizotinib-untreated group did not reach the median. Although ceritinib could salvage crizotinib-refractory patients temporally, recurrence was observed earlier in these individuals compared with crizotinib-naï ve patients. Thus, studies of ceritinib resistance will be very important. A preclinical study reported that ceritinib was sensitive to cell lines expressing ALK with ALK-TKIs-resistant mutations (L1196M, I1171T, S1206Y and G1269A), but could not inhibit the growth of cells with G1202R and F1174C mutations in ALK [46]. In a phase 1 study, ALK secondary mutations (1151Tins, L1196M or G1269A) were detected in refractory tumors after crizotinib-naï ve patients were treated with ceritinib. These results suggest that ceritinib could overcome resistance by acquiring L1196M or G1269A mutations, whereas ceritinib might paradoxically cause these mutations. In addition, a preclinical study showed that ceritinib could overcome alectinib resistance, including in tumors with ALK secondary mutations (I1171T and V1180L) [51]. In contrast, preclinical studies demonstrated that the resistance induced by L1196M and G1269A mutations which could occur in patients with ceritinib-resistance could be overcome by alectinib [44,45]. Therefore, alectinib might be useful in ceritinib-refractory patients. 4. Alectinib Alectinib is a selective and potent ALK inhibitor [44] that was approved in Japan on 4 July 2014. A recent study also revealed that the drug inhibited RET kinase [52]. In a Japanese phase 2 trial of ALK inhibitor-naïve patients with ALK-rearranged advanced NSCLC, alectinib achieved an excellent response rate of 93.5%, a long response duration, and an acceptable toxicity profile [28]. A total of 19.6% of patients achieved a complete response, and the 2-year progression-free survival rate was 76%. There was no progression in CNS lesions among patients with known CNS metastases at baseline [29]. A study performed in the United States in ALK-positive NSCLC patients treated previously with crizotinib (but not with other ALK inhibitors) demonstrated a good ORR of 54.5%. The preliminary results also suggested that alectinib might have antitumor activity against CNS lesions in patients with crizotinib-refractory disease [53]. Therefore, the FDA granted alectinib a “breakthrough therapy” designation for patients with metastatic NSCLC that progressed after treatment with crizotinib in 2013. A preclinical study demonstrated the efficacy of alectinib in intracranial metastases. Specifically, treating a mouse xenograft model formed from H2228 cells with alectinib resulted in regression of the brain tumors and provided a survival benefit. In a pharmacokinetic study in rats, alectinib achieved a high brain-to-plasma ratio [54]. Actually, some crizotinib-refractory patients with CNS metastasis had response to alectinib [55–58]. Alectinib also had substantial inhibitory potency against tumors with ALK secondary mutations (addition to gatekeeper mutation L1196M, 1151Tins, L1152R C1156Y, F1174L and G1269A). However, alectinib seems to be less potent against ALK G1202R mutations [45,59]. Katayama et al. discovered the V1180L ALK gatekeeper mutation in vitro and an I1171T ALK mutation in a tumor from a patient treated with crizotinib followed by alectinib. In another report, a patient with alectinib-resistance harbored the I1171T ALK mutation [60]. In addition, ALK-positive tumors harboring V1180L and I1171T mutations could be suppressed by the second-generation ALK inhibitors, ceritinib, AP26113, and ASP3026. Ceritinib and AP26113 were effective at inhibiting tumors expressing the V1180L and I1171T mutations, whereas ASP3026 seemed to be inactive against I1171T

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Ba/F3 murine cells [51]. As well as crizotinib-resistance, we would have to select the suitable drug in each alectinib-refractory patient with the ALK secondary mutation. MET amplification was observed in alectinib-refractory tumors, although tumor samples from before the alectinib treatment were not available [31]. The patient experienced a dramatic response to crizotinib, which strongly inhibits MET tyrosine kinase. Moreover, MET activation induced by hepatocyte growth factor (HGF) in an autocrine manner was observed in an alectinib-resistant cell line [61]. The cells were sensitive to crizotinib both in vitro and in vivo. Thus, crizotinib might be effective against crizotinib-naï ve tumors expressing MET activation after treatment with alectinib. Two randomized clinical trials comparing alectinib with crizotinib in the first-line setting are ongoing (J-ALEX and ALEX studies). In addition, a phase 2 study (UMIN000015984) of crizotinib monotherapy in patients with alectinib-refractory NSCLC harboring EML4-ALK would be interesting. 5. Other Novel ALK Inhibitors In addition to ceritinib and alectinib, other second-generation ALK inhibitors (AP26113, ASP3026, TSR-011, PF-06463922, RXDX-101, X-376, X-396, CEP-28122, and CEP-37440) have been developed to date (Table 3) [62]. These new agents are expected to exhibit efficacy in the CNS and might help overcome drug resistance. Table 3. ALK inhibitors. Drugs

Company

Other activity

Clinical trials

Crizotinib (PF02341066)

Pfizer

MET, ROS1

Phase 1, 2, 3

Ceritinib (LDK378)

Novartis

IGF1R, INSR

Phase 1, 2, 3

Alectinib (CH5424802)

Chugai, Roche

RET

Phase 1, 2, 1/2, 3

AP26113

Ariad

EGFR, ROS1

Phase 1/2

ASP3026 X-376 X-396

Astellas Xcovery

Phase 1 Phase 1 (X-396)

TSR-011

Tesaro

RXDX-101

Ignyta

ROS1 MET TRK-A, TRK-B, TRK-C ROS1 TRK-A, TRK-B, TRK-C

CEP-28122 CEP-37440 PF-06463922

Teva Pfizer

Phase 1/2a Phase 1

RSK2, RSK3, RSK4 Phase 1 (CEP-37440) ROS1

Phase 1/2

Status Approved by FDA (Auguet 2011) Clinically available in Japan (March 2012) Approved by FDA (May 2014) Breakthrough Therapy Designation (June 2013) Clinically available in Japan (July 2014) Breakthrough Therapy Designation (October 2014)

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AP26113 is a potent TKI that can inhibit both ALK, EGFR and ROS1 [63]. In an ongoing phase 1/2 study of AP26113 (NCT01449461), patients with ALK-positive NSCLC resistant to crizotinib demonstrated an ORR of 67%. Four of five patients with ALK-positive CNS metastatic lesions experienced tumor regression [64]. In a preclinical study using Ba/F3 cell lines expressing clinically identified EML4-ALK mutations, AP26113 was effective on crizotinib- or alectinib-resistant mutations (L1196M, V1180L, and I1171T) [63]. A phase 1 study of ASP3026, which inhibits ALK, ROS1, ACK and EGFR in vitro, showed a favorable safety profile in patients with advanced solid tumors despite ALK positivity (NCT01401504). The tumor response has not been reported [65]. As described previously, ASP3026 was effective in tumors expressing resistance mutations, including the ALK gatekeeper mutations L1196M in crizotinib-resistant and V1180L in alectinib-resistant preclinical models [51]. PF-06463922 is a small ATP competitive inhibitor of ALK/ROS1 that penetrates the blood-brain barrier in preclinical animal models. Thus, it exhibits high activity in crizotinib-resistant cells [66]. A phase 1/2 study in patients with ALK-positive and ROS1-positive NSCLC is ongoing (NCT01970865). X-376 and X-396, which suppress the kinase activity of ALK and MET, were developed. In contrast, crizotinib was shown to be a slightly more potent MET inhibitor than X-376 or X-396. In addition, X-396 could inhibit the crizotinib-resistant mutants L1196M and C1156Y potently [67]. Recently, mechanisms of resistance to X-376 were reported in a resistant cell line model (H3122 XR) established from parental H3122 cells. H3122 XR exhibited increased IGF-1R phosphorylation when IGF-1R was overexpressed. H3122 XR cells were sensitive to the combination of an IGF-1R inhibitor or anti-IGF-1R antibody and X-376 [37]. A phase 1 trial of X-396 is ongoing in patients with advanced solid tumors (NCT01625234). TSR-011 is a potent ALK-TKI. Although a phase 1/2 study (NCT02048488) is ongoing, four of six evaluable ALK-positive and crizotinib-pretreated patients achieved a response. Interestingly, the drug exhibited activity against tropomyosin-related kinase (TRK) A, B, and C receptor (encoded by NTRK1, NTRK2, and NTRK3, respectively) both in vitro and in vivo [68]. Because the driver oncogene of NTRK1 in NSCLC has been reported [69], the effects of TSR-011 in NSCLC with TRK rearrangements should be explored further. RXDX-101 also inhibits ALK, ROS1, TRK-A, TRK-B, and TRK-C. It is active against the ALK mutants responsible for crizotinib resistance (L1196M and C1156Y) and crosses the blood-brain barrier in a mouse brain metastatic model [70,71]. In an ongoing phase 1 study, NSCLC and neuroblastoma patients with ALK mutations, NSCLC patients with ROS1 mutations, and colorectal cancer patients with TRK-A mutations achieved a response without dose-limiting toxicity (NCT02097810) [72]. CEP-28122 is a selective active ALK inhibitor with favorable pharmaceutical and pharmacokinetic profiles against ALK-positive tumors in vitro and in vivo [73]. Although it has not been in clinical development, a phase 1 trial (NCT01922752) is ongoing using CEP-37440, which inhibits both ALK and focal adhesion kinase (FAK); however, the details have not yet been reported. 6. Conclusions and Future Therapeutic Strategies Since the FDA approved crizotinib in 2011, understanding of its efficacy, toxicity, and resistance has increased. Crizotinib is a key drug in the current therapeutic strategy for ALK-positive lung cancer. However, it has a limited response duration, and drug resistance must be considered. In addition, crizotinib has limited efficacy in the CNS. Therefore, second generation ALK-TKIs are required.

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Because there are a variety of crizotinib resistance mechanisms, various mechanisms should be assessed using resistant samples to provide appropriate treatment for patients. The most common mechanism of crizotinib resistance is a series of ALK secondary mutations. Unlike gefitinib resistance, ALK secondary mutations are miscellaneous, because each mutation has a different sensitivity to different second-generation ALK inhibitors. If a crizotinib-refractory patient has the L1196M ALK gatekeeper mutation, alectinib or an HSP90 inhibitor might be effective. In contrast, ceritinib, alectinib, AP26113, or PF-06463922 might be preferred in crizotinib-refractory patients with brain metastasis. In cases of crizotinib resistance involving EGFR or IGF-1R, ASP3026 (a dual ALK/EGFR inhibitor) or ceritinib (which inhibits ALK and IGF-1R) would be reasonable. Although crizotinib has achieved a higher response rate than chemotherapy in first- and second-line setting phase 3 trials, the OS between the crizotinib-arm and chemotherapy-arm are similar so far. In the second-line study, crizotinib was superior to chemotherapy (ORR, 65% vs. 20%, respectively; PFS, 7.7 months vs. 3.0 months) [25]. In the first line study, standard chemotherapy in ALK-positive patients achieved a good response (ORR, 74% in crizotinib-arm vs. 45% in chemotherapy-arm; PFS, 10.9 months vs. 7.0 months) [26]. The similar OS between the two arms might be a result of the effective treatment achieved using chemotherapy. Thus, advanced ALK-positive NSCLC patients should be treated with both ALK-TKIs and chemotherapy if they have a good performance status. Actually, there were so-called “super-responders” to pemetrexed among ALK-positive patients [25]. When a biomarker confirming the efficacy of pemetrexed in selected patients is identified and confirmed, chemotherapy based on precision medicine could be achieved. Although treatment with crizotinib is standard today, alectinib is also likely to be administered in the first line setting. In the near future, crizotinib or ceritinib might be selected in alectinib-refractory patients with activated MET or secondary mutations (V1180L or I1171T). In addition, alectinib might be acceptable in frail patients who are elderly or have a poor performance status, because the adverse effects of alectinib seem to be milder compared with those associated with crizotinib. On February 2, 2015, the anti-PD-L1 agent MPDL3280A received a breakthrough therapy designation from the FDA as a potential treatment for patients with PD-L1-positive NSCLC who progressed on platinum-based chemotherapy and an EGFR or ALK inhibitor. This suggests that immunotherapy might be useful for ALK-positive NSCLC patients who were treated with or without ALK-TKIs. Subsequently, the FDA granted approval to the anti-PD-1 agent nivolumab for the treatment of patients with metastatic squamous NSCLC with progression on or after platinum-based chemotherapy on March 4, 2015. PD-1 positive tumor infiltrating lymphocytes and PD-L1 expressing tumor cells were seen in 18 of 42 cases (43%) of which 8 cases lacked other biologic targets including EGFR mutations, HER2, cMET, ALK, or ROS1 rearrangements [74]. In addition, PD-L1 expression was high in NSCLC tumors with ALK translocations (n = 10) [75]. Studies on immune activation/T cell infiltration in response to ALK inhibitors will be needed in the future. In conclusion, the mechanisms of acquired resistance to ALK inhibitors are just beginning to be understood. They should be exactly elucidated. New molecular-targeted drugs and their mechanisms of resistance must be understood in detail, which would allow the appropriate therapeutic strategies to be applied.

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Conflicts of Interest Nagio Takigawa has received honoraria from Pfizer Inc. Japan, and Chugai Pharmaceutical. Katsuyuki Kiura has received honoraria from Pfizer Inc. Japan, Chugai Pharmaceutical, and Novartis Pharmaceuticals Japan. Other author declared no conflict of interest relevant to this study. References O’Brien, S.G.; Guilhot, F.; Larson, R.A.; Gathmann, I.; Baccarani, M.; Cervantes, F.; Cornelissen, J.J.; Fischer, T.; Hochhaus, A.; Hughes, T.; et al. Imatinib compared with interferon and low-dose cytarabine for newly diagnosed chronic-phase chronic myeloid leukemia. N. Engl. J. Med. 2003, 348, 994–1004. 2. Deininger, M.; O’Brien, S.G.; Guilhot, F.; Goldman, J.M.; Hochhaus, A.; Hughes, T.P.; Radich, J.P.; Hatfield, A.K.; Mone, M.; Filian, J.; et al. International randomized study of interferon vs. sti571 (IRIS) 8-year follow up: Sustained survival and low risk for progression or events in patients with newly diagnosed chronic myeloid leukemia in chronic phase (CML-CP) treated with imatinib. Blood 2009, 114, Abstract 1126. 3. Paez, J.G.; Janne, P.A.; Lee, J.C.; Tracy, S.; Greulich, H.; Gabriel, S.; Herman, P.; Kaye, F.J.; Lindeman, N.; Boggon, T.J.; et al. EGFR mutations in lung cancer: Correlation with clinical response to gefitinib therapy. Science 2004, 304, 1497–1500. 4. Lynch, T.J.; Bell, D.W.; Sordella, R.; Gurubhagavatula, S.; Okimoto, R.A.; Brannigan, B.W.; Harris, P.L.; Haserlat, S.M.; Supko, J.G.; Haluska, F.G.; et al. Activating mutations in the epidermal growth factor receptor underlying responsiveness of non-small-cell lung cancer to gefitinib. N. Engl. J. Med. 2004, 350, 2129–2139. 5. Maemondo, M.; Inoue, A.; Kobayashi, K.; Sugawara, S.; Oizumi, S.; Isobe, H.; Gemma, A.; Harada, M.; Yoshizawa, H.; Kinoshita, I.; et al. Gefitinib or chemotherapy for non-small-cell lung cancer with mutated EGFR. N. Engl. J. Med. 2010, 362, 2380–2388. 6. Soda, M.; Choi, Y.L.; Enomoto, M.; Takada, S.; Yamashita, Y.; Ishikawa, S.; Fujiwara, S.; Watanabe, H.; Kurashina, K.; Hatanaka, H.; et al. Identification of the transforming EML4-ALK fusion gene in non-small-cell lung cancer. Nature 2007, 448, 561–566. 7. ALK anaplastic lymphoma receptor tyrosine kinase [homo sapiens (human)]. Available online: http://218.245.4.153/cgi-bin/orf/gene.pl?geneid=238&species=9606 (accessed on 20 March 2015). 8. Morris, S.W.; Kirstein, M.N.; Valentine, M.B.; Dittmer, K.G.; Shapiro, D.N.; Saltman, D.L.; Look, A.T. Fusion of a kinase gene, ALK, to a nucleolar protein gene, NPM, in non-hodgkin’s lymphoma. Science 1994, 263, 1281–1284. 9. Griffin, C.A.; Hawkins, A.L.; Dvorak, C.; Henkle, C.; Ellingham, T.; Perlman, E.J. Recurrent involvement of 2p23 in inflammatory myofibroblastic tumors. Cancer Res. 1999, 59, 2776–2780. 10. Mosse, Y.P.; Laudenslager, M.; Longo, L.; Cole, K.A.; Wood, A.; Attiyeh, E.F.; Laquaglia, M.J.; Sennett, R.; Lynch, J.E.; Perri, P.; et al. Identification of ALK as a major familial neuroblastoma predisposition gene. Nature 2008, 455, 930–935. 1.

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