Vol. 59, No. 3
INFECTION AND IMMUNITY, Mar. 1991, p. 1148-1152 0019-9567/91/031148-05$02.00/0 Copyright © 1991, American Society for Microbiology
Mechanism of Action of Moraxella bovis Hemolysint KENNETH D. CLINKENBEARD* AND AMY E. THIESSEN
Department of Veterinary Pathology, College of Veterinary Medicine, Oklahoma State University, Stillwater, Oklahoma 74078 Received 3 October 1990/Accepted 6 December 1990
Bovine erythrocytes (RBCs) exposed to Moraxella bovis culture supernatants exhibited rapid leakage of intracellular K+ (95% in 10 min), slower cell swelling (1.20-fold increase in mean corpuscular volume in 20 min), and subsequent lysis (76% leakage of hemoglobin in 25 min). Incubation media made hypertonic by the addition of 75 mM carbohydrates with molecular diameters of 0.72 to 1.32 nm prevented hemolysin-induced RBC swelling, but incubation media made hypertonic by the addition of carbohydrates with molecular diameters of
INFECTION AND IMMUNITY, Mar. 1991, p. 1148-1152 0019-9567/91/031148-05$02.00/0 Copyright © 1991, American Society for Microbiology
Mechanism of Action of Moraxella bovis Hemolysint KENNETH D. CLINKENBEARD* AND AMY E. THIESSEN
Department of Veterinary Pathology, College of Veterinary Medicine, Oklahoma State University, Stillwater, Oklahoma 74078 Received 3 October 1990/Accepted 6 December 1990
Bovine erythrocytes (RBCs) exposed to Moraxella bovis culture supernatants exhibited rapid leakage of intracellular K+ (95% in 10 min), slower cell swelling (1.20-fold increase in mean corpuscular volume in 20 min), and subsequent lysis (76% leakage of hemoglobin in 25 min). Incubation media made hypertonic by the addition of 75 mM carbohydrates with molecular diameters of 0.72 to 1.32 nm prevented hemolysin-induced RBC swelling, but incubation media made hypertonic by the addition of carbohydrates with molecular diameters of
