432 reviews
$6 Stoning,P. L. (1989) Smd.j. Clir!.I&. Iwws~. 49 (Soppl.I93), 34-42 47 Seth,J.. Hanning, 1.. Bacoi:.1:. Ilways been an important aspect of biopolymer characterization. Molecular-weight measurements have been used to establish identity, analyse quaternary structure (i.e. the presence ofsubunits), prove homogeneity (i.e. the absence ofother biopolymers) and to detect moditicatiom su+ as glycosylation or proteolysis. Most molecular-mass estimates are made using sodium dodecyl sulphatc polyacrylamide gel electrophoresis (SDS-PAGE), or gel-permeation chromatography (GPC), calibrated with known standards. Both techniques are able to $ve useful indications of purity, relative molecular masses and approximate amounts of biopolymer present, by comparison among the different samples that the techare run alongside sample material. However, niques are unable to provide the mass accuracy and resolution really needed by the analyst. For example, loss of a few terminal amino acids tiom a pr?rr. ; or its modification by a single small mucin chain
$6 Stoning,P. L. (1989) Smd.j. Clir!.I&. Iwws~. 49 (Soppl.I93), 34-42 47 Seth,J.. Hanning, 1.. Bacoi:.1:. Ilways been an important aspect of biopolymer characterization. Molecular-weight measurements have been used to establish identity, analyse quaternary structure (i.e. the presence ofsubunits), prove homogeneity (i.e. the absence ofother biopolymers) and to detect moditicatiom su+ as glycosylation or proteolysis. Most molecular-mass estimates are made using sodium dodecyl sulphatc polyacrylamide gel electrophoresis (SDS-PAGE), or gel-permeation chromatography (GPC), calibrated with known standards. Both techniques are able to $ve useful indications of purity, relative molecular masses and approximate amounts of biopolymer present, by comparison among the different samples that the techare run alongside sample material. However, niques are unable to provide the mass accuracy and resolution really needed by the analyst. For example, loss of a few terminal amino acids tiom a pr?rr. ; or its modification by a single small mucin chain
