Lymphangiomyomatosis in the Atlantic Bottlenose Dolphin (Tursiops truncatus) Author(s): Arnold J. Rawson, M.D., Geoffrey W. Patton, B.S., John S.J. Brooks, M.D. Source: Journal of Wildlife Diseases, 28(2):323-325. Published By: Wildlife Disease Association DOI: http://dx.doi.org/10.7589/0090-3558-28.2.323 URL: http://www.bioone.org/doi/full/10.7589/0090-3558-28.2.323
BioOne (www.bioone.org) is a nonprofit, online aggregation of core research in the biological, ecological, and environmental sciences. BioOne provides a sustainable online platform for over 170 journals and books published by nonprofit societies, associations, museums, institutions, and presses. Your use of this PDF, the BioOne Web site, and all posted and associated content indicates your acceptance of BioOne’s Terms of Use, available at www.bioone.org/page/terms_of_use. Usage of BioOne content is strictly limited to personal, educational, and non-commercial use. Commercial inquiries or rights and permissions requests should be directed to the individual publisher as copyright holder.
BioOne sees sustainable scholarly publishing as an inherently collaborative enterprise connecting authors, nonprofit publishers, academic institutions, research libraries, and research funders in the common goal of maximizing access to critical research.
Journal
of Wildlife
Diseases,
Lymphangiomyomatosis (Tursiops
in the Atlantic
M.D.,’
Arnold J. Rawson,
The
ABSTRACT:
a
Geoffrey
first
in a marine
stranded
male
( Tursiops
disease
diastinal have
of lymphangiomyis reported
Atlantic
been
bottlenose This
this
of
lymph
of testosterone
ported
cases
females. Key dolphin, terone,
words: Tursiops truncatus, lymphangiomyomatosis, first case, case report.
phangio!eiom
yomatosis)
mon non-malignant proliferative process, case studies have been (Enzinger and Weiss, composed muscle nary
is an
lymph
vessels
parenchymal
nodes,
and
lymph
nodes
masses
and may
as
involve
as well.
The
is
involvement
may
fuse.
Affected
individuals
dyspnea
and
effusion cases
often
The
smooth
collection
muscle
in
1988),
ceptors on the (Brentani Exposure
for both estrogen smooth muscle et a!., 1984; to estrogens
sion as
of the
and
lesion
ovaniectomy,
one administration (McCarty et a!.,
1984).
females
Weiss,
or
studies
(sleek
dif-
ter
pleura! and
shown
who
building
went
(27#{176}10’35”N, 82#{176}29’50”W). saw the animal wash into into the water and moved
and
shore while authorities.
treatment.
animal
exhibited
with
only
slightly
foul
of brown
nasal
attempt)
and
and
bony
superficial breath,
exudate mild
small (1.0 cm). Respirations were regular and averaged 1 to 3/mm with a heart nate of 66 bpm. The dolphin was physiologically stabilized through rehydration and medication. Af-
with
(Enzinger
Key
abrasions amounts
intractable
et a!.,
been
was
retroperitonea!
nodular
re-
dolphin (Tursiops truncatus) As Tropical Storm Marco
evaluation
lymph
present
with
(Graham have
be
previous mammals. of lymphanbottlenose
pulmo-
cells are arranged about endothelium-lined spaces. Foci of lymphocytes and hemosidenin deposits may be seen throughout (Enzingen and Weiss, 1988). Involved lungs show evidence of bronchial obstruction. The
no
The animal was transported by stranding network personnel to Mote Marine Laboratory (Sarasota, Florida 34236, USA) and was placed in a natural seawater tank for
of smooth
mediastinal
contains
the animal to a nearby sandy another person went to notify
uncom-
within
literature
‘Mote Marine Laboratory, 1600 Laboratory Medicine, University
port of this !esion in marine Hence, the following report giomyomatosis in the Atlantic
on Casey A beachgoer the rocks
but life-threatening of which numerous reported in humans 1988). The lesion
of proliferating and
known
and
off Florida’s west coast the evening of 10 October 1990, a 295 cm, 193 kg male do!phin was found alive at a rocky gulf shore
bottlenose lung, testos-
(also
Lymphangiomyomatosis !ym
me-
nodes.
may significant since all redisease in humans occur in
pathologically
level
pro!if-
the
and
M.D.,2
of Pathology
The
dolphin
lungs
mesenteric
low
John S.J. Brooks,
from
progressive
the
probably
An extraordinarily
B.S.,’
case
involved
and
W. Patton,
florida 34236, USA; 2 Department Pennsylvania 19104, USA
mammal
truncatus).
erative
Dolphin
truncatus)
Thompson Parkway, Sarasota, of Pennsylvania, Philadelphia,
omatosis
Bottlenose
1992. pp. 323-325 Association 1992
28(2), Disease
© Wildlife
re-
and progesterone cells of the lesions Co!ley et a!., 1989). speeds the progres-
ing mal
an examination died and was
the
following
was
taking
food
day five, it was dedolphin to another recuperation. Dur-
on day examined
nine, the post-mortem
ani-
day.
At necropsy the lungs were described as !umpy and somewhat hard to cut. Lange firm areas suggestive of neoplasm were present and scattered cystic change was seen in the surrounding parenchyma. On
(Shen et al., 1987), whereand especially progesterpermit regression 1980).
section 323
the
firm
areas
consisted
of
uni-
JOURNAL
324
OF WILDLIFE
DISEASES,
VOL.
28, NO.
2, APRIL
1992
..1
,..
..-
-
.
FIGURE
.
1.
bottlenose showing
Section
dilated
surrounded 320
of a lung
dolphin by
with lymphatic
smooth
nodule
in an
Atlantic
channels muscle
of
various
bundles.
2.
FIGURE
bottlenose
lymphangiomyomatosis H&E.
sizes Bar
showing
smooth
lymphatic the
sm.
pale grayish were large dark
tissue, throughout foci, which proved
on microscopy Mediastina!
to be hemosiderin lymph nodes measured
in diameter firm grayish
and were composed tissue with dark
deposits.
Mesenteric
lymph
sured grossly
3 cm in diameter. normal; testicular
saved seen growth
for microscopy. in the other layer groups
and
in relation of small
scattered
Bar
in an
Atlantic to dilated
lymphocytes
lymphocytes
in in
the
80 sm.
firm areas, replaced.
lung There
parenchyma was were numerous
nodes
their
(Armed Washington, Number
3). In histologic
of lung
mea-
appeared was not
this animal specimens the Registry
Comparative Pathology Institute of Pathology, 20306, USA; Accession sections
the pale entirely
nodules clusters
interstitium
nodule
lymphangiomyomatosis
of similar hemosidenin
No abnormality organs. Counting (GLG) in the
(Hohn et a!., 1989) showed >20-yr-old. Representative this lesion were sent to
muscle
H&E.
lung
short fasciculi of smooth musc!e throughout. The muscle bundles were distinguished from collagen by means of the Masson Trichrome stain (Masson, 1929) and
Testes tissue
deposits. 4 cm
of
with
channels,
channel.
formly which
Section
dolphin
was of teeth was of of
Forces D.C. 2304654taken
from
nature
was
positive reaction muno-peroxidase Hrapchak, formed
confirmed
In some
1987).
small
by
for desmin technique nodular
means
of a
using the (Sheehan
imand
areas aggregates,
the
muscle while
elsewhere it surrounded small endothelium-lined vascular channels which contained proteinaceous fluid and occasional lymphocytes but no red blood cells. Between these channels ies filled with red foci were
were blood capillarblood cells. Numerous
of lymphocytes and scattered throughout
of hemosidenin the tissue (Figs.
SHORT
1, 2). In the lung parenchyma nipheny of the lesions were many dilated there
was
bronchi. patchy
at the pecystical!y
Elsewhere in the acute bronchopneumo-
lung
nia.
vived
and
the
amination.
COMMUNICATiONS
subsequent
We
325
post-mortem
particularly
asota Memorial Hospital Pathology Laboratory for
ex-
thank
the
and
Sarasota the lab
help
with
San-
analyses. Sections
vealed
mediastinal little remaining
of
very
structure.
The
placed
by
sels,
node
musc!e
was
similar of the
areas node, served.
the
and
to those lung. In
node However,
nodes relymph node
almost
fascicu!i,
lymphocytes,
posits
lymph
tota!ly
lymphatic hemosiderin
seen in the a mesentenic
structure there
was was
largely a focal
crease in smooth muscle surrounding phatic vessels, suggestive of involvement. Hormonal assays performed on serum
collected
the
first
estrogen