BIOLOGY

OF

(1990)

REPRODUCTION

43,

Luteinizing

Hormone-Enhanced

784-787

with

and without

KURT

Department

A. ZUELKE

of Physiology The

Maturation

Protein

Supplementation

and

and

University

In Vitro

BENJAMIN

G.

Pharmacology, of Georgia,

Oocytes

BRACKETF1

College Athens,

of Bovine

of Veterinaiy

Georgia

Medicine

30602

ABSTRACT Luteinizing

hormone

by elevated

proportions

proportions

of ova

bLH-B-5,

100

proestrous

was

were

g/mlJ)

(Day

20)

shown

of oocytes fertilized than bovine

fertilized

in vitro

In TCM-199 serum

maturation

to enhance that

replaced

and

after

in vitro

(

alone the

< BSA,

of immature

reached

maturation

0.01). but

stages

(IVM)

Further similar

improvement

from

obtained in

vitro

after

modified

in

proportions

blastocysts.

into

oocytes

blastocyst

TCM-199 in 1W

(p

+ BSA followed

0.005)

(22.2%

ova

-

.

cattle

fertilization

(TCM-199