Lung Lesions in an Opossum (Didelphis virginiana) Associated with Capillaria didelphis Author(s): Daniel E. Snyder, Amir N. Hamir, Cathleen A. Hanlon, and Charles E. Rupprecht Source: Journal of Wildlife Diseases, 27(1):175-177. Published By: Wildlife Disease Association DOI: http://dx.doi.org/10.7589/0090-3558-27.1.175 URL: http://www.bioone.org/doi/full/10.7589/0090-3558-27.1.175
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Journal
Lung
Lesions
in an Opossum
Associated
with
Capillaria
(Dideiphis
of Wildlife
Diseases,
1991,
27(1),
pp.
175-177
virginiana)
dideiphis
Daniel E. Snyder,’ Amir N. Hamir,2 Cathleen A. Hanlon,3 and Charles E. Rupprecht,3 ‘U.S. Department of Agriculture, Agricultural Research Service, Animal Parasite Research Laboratory, POB 952, Auburn, Alabama 36831, USA; 2 University of Pennsylvania, School of Veterinary Medicine, New Bolton Center, 382 West Street Road, Kennett Square, Pennsylvania 19348-1692, USA; 3The Wistar Institute of Anatomy and Biology, 3601 Spruce St., Philadelphia, Pennsylvania 19104, USA
(Dideiphis virgion Parramore Island, Virwith the lung parasite Capillaria dideiphis. Lung lesions associated with this parasite were extensive and consisted of flumerous areas with central necrosis deep within the parenchyma. Necrotic foci were present around airways and consisted of a mixture of inflammatory cells and cellular debris, adult nematodes and many bipolar eggs. This parasite causes severe verminous pneumonia in the opossum. Key words: Capillanla dideiphis, nematode, ABSTRACT: An nl,ana) live-trapped ginia was infected
opossum,
Dideiphis
monary,
Virginia,
opossum
virginiana,
case
pathology,
line
data
collection
plete
necropsy
Except
been
found
in
the
or
organs
turgida,
spaces and within foci of the lungs virginiana)
or necrotic (Didelphis
southeastern
and
and
eosin
examination
eastern
in
pulmonary
sive
and
within
the
crotic
foci,
a mixture
and and
cellular many
proposed didelphis,
2).
Necrotic and
C.
Harkema,
a new species name, for this parasite, in
aerophilia
that
Capillaria contrast
is commonly
the trachea and bronchi vulpes) and other wild
1970;
found
of red fox carnivores.
to
ways
in
fected could
(Vulpes A pre-
phostrongylus wood et a!.,
1977).
hayesi This
The ramore
opossum Island,
75#{176}38’W) in
was August
of
an
live-trapped
Virginia 1989
tory
(USA: as part
Par-
sue
37#{176}32’N,
were
prominent action.
of base175
necrosis In
the
ne-
of inflammatory sections eggs were
cells
of nematodes, seen (Figs.
foci were present in some of the less
around severely
1, airaf-
bronchi ulcerated
3). Elsewhere in the lung, at from the necrotic foci, bron-
and
filled
marked rounding vessels.
opossum infected on
cells,
sized
central
parenchyma.
debris, bipolar
exten-
variably
areas, outlines of dilated be identified with focally
eggs
and
quite
many with
chioles were dilated, cosa, and a mixture
infections (Prestreport further de-
scribes lesions in the lung naturally and monospecifically with C. didelphis.
3 mm)
mucosa (Fig. some distance
vious report described the histopathologic lesions in the lung of opossum associated with concomitant C. didelphis and Didel-
sectioned microscopy.
were
of
deep
and
major
buffered
hematoxylin
lesions
Chitwood and Lichtenfels, 1972; Feldman et a!., 1972; Nettles et a!., 1975; Pnestwood et a!., 1977; Gandiner, 1982; Brown, 1988). Butterworth and Bevenley-Bunton (1977)
(Miller
of all
lesions were seen in the stomach tissue sections.
the
(1
States
the
gross lecarcass.
the
in
by light
areas
United
Turgida
neutral
with
consisted to
in
and
in paraffin,
stained
The
species
sections
in 10%
animal
other
seen
embedded
at 5 sm
Histopathologic lungs and
alveolar
gnanulomata of opossum
in the
have
fixed
vac-
to euthanasia.
no
were
tissue
for
The
prior
americana
parasites
were
rabies
et al., 1986). by an intraand a com-
nematode
Representative
pul-
sp.
two Cruzia
(Physaloptera) sions
as Cap-
bronchioles,
to oral
performed.
normal
for
stomach,
fonmalin, identified Capillaria
was
clinically
was
report.
Capi!lariid parasites illaria aerophili.a or
prior
cine field trials (Ruppnecht The animal was euthanized venous barbituate overdose
had hyperplastic muof mucus, inflamma-
bipolar
the
plugged
lumina.
nematode
There
was
also
hyperplasia of smooth muscle surbronchioles and associated blood Bronchus-associated lymphoid tispresent;
however,
feature Endogenous
of
the lipid
this
was
pulmonary pneumonia
not
a reas
176
JOURNAL OF WILDLIFE DISEASES, VOL. 27, NO. 1, JANUARY
FIGURE
delphis
1.
of parasites,
and
Low
induced
cellular
magnification
pulmonary eggs,
debris.
and
a mixture
Bar
of
lesion
=
200
di-
Capillanla
containing
1991
sections
of inflammatory
cells FIGURE
m.
3.
bris.
Dilated
bronchiole
didelphis
Capillanla
Mucosa
ulcerated.
and
of the Bar
=
described by with verminous in this
dilated
50
consisted
mucosal necrosis
Gastric
granulomata containing of adult
were
lung
tures
and
measurements
and
are FIGURE 2. Caplllanla dideiphis adults, eggs, inflammatory cells and cellular debris within monary parenchyma. Bar = 50 Mm.
and pul-
were
dissected
morphologic of these
corresponded
to the
given by (1977).
feaparasites
description
Buttenworth Representative
and
a porparasites National
Collection (Beltsvil!e, Maryland USA; accession number 81194). microscopic lesions described above
similar
to those
et a!. (1977). inflammatory didelphis
subareas of nema-
specimens of C. didelphis, including tion of formalin fixed lung with in situ, are deposited in the U.S. Parasite 20705, The
minor
eosinophilic
worms
fixed
eggs
of de-
is partially
with central degenerating
the
of C. didelphis Beverley-Burton
and
in opossums was not seen
lesions
a few
from and
bronchiole
Brown (1988) pneumonia of
todes. Portions
eggs
cells
m.
opossum.
and
containing
inflammatory
causes
reported
As these reaction extensive
by Prestwood
authors noted, the in response to C. damage
to the
SHORT
pulmonary
panenchyma.
monic
by this
damage
comitant sites can
burden be lethal
opossum animal
et
1969;
Vaki!zadeh
1979).
Opossum
may
harbor
and
associated
scribed aware their
in
has been used in several studies
C.
in the by
1971;
are
wild
other
pulmonary
lesions
D.
Hayes
for
field.
This
work
the
Jurcaught
Animal
as
C.
parasites erinary
was
Pennsylvania
debe
supported
News
Society
C. C.
BROWN,
1988.
in opossums Diseases
Endogenous
from
24:
of
Washington
37:
of
Severe
Wildlife
PRESTWOOD, FARREL. perimentally
and
Journal
Florida.
recombinant
E. W., AND M. BEVERLEY-BURTON. Capillanla didelphis n. sp. (Nematoda: Trichuroidea) from the opossum, Didelphis emginiana L. in Georgia. Canadian Journal of Zo-
BUTTERWORTH, 1977.
ology
55:
616-619.
M.,
CHITWOOD,
AND
Identification tions.
Experimental
J. H. parasitic
L.
helminths
1972.
SELF.
of
the
metazoa
in tissue 32:
M.
W.
Characteristics Virginia
1972.
LICHTENFELS.
Parasitology
D. B., J. A. MOORE,
FELDMAN,
J.
of
with
opossum
sec-
407-519. HARRIS,
virus
of
A.
HAMIR,
L.
T.
1986.
Oral
immu-
lo-
glycoprotein
Proceedings
Sciences
B.
CLICK-
(Procyon
rabies of
of Vet-
N.
of raccoons vaccine.
L.
of ex-
lungworm Journal
of
USA
83:
B. F., D. T. ROWLAND,
AND
J. C.
phis
emrgmniana,
ratory
Animal
as a
Care
J., B.
VAKILZADEH, AND
1969.
LEMAY.
J. C.
pulmonary
F.
LEMAY. adenomas Laboratory
The
the
7947-
D. B. HACKEL, Didel-
opossum,
laboratory
19:
animal.
SHERWOOD, 1971.
D.
B. HACKEL,
Experimental
in the
study
Animal
for
publication
7 February
of
(Didelphis
opossum Science
228.
Received
Labo-
494-499.
common
(Didelphis
H.
7950. SHERWOOD,
virgmniana). AND
acquired
vaccinia
Academy
419-420.
WUNNER,
protection a
National
H.
DA-
in an opossum. 11:
H. KOPROWSKI.
and
of
Wildlife
W.
North
W. H.
AND
529-532. WIKTOR,
C. E., T. J.
in
Helminthological
American
38:
Helminths
NETTLES, AND manifestations
naturally
Research
AND
Re-
36-39.
Diseases
in opossums.
tor)
the
parasitism
A. K., V. F. 1977. Pathologic
pneumonia
214-219.
Animal
18-21.
1975.
VIDSON.
nization
lipid
as a laboratory
F., A. K. PRESTWOOD,
V.
Vetof Pa-
p. 31.
marsupial
Proceedings of
NETTLES,
MAN,
CITED
Institute
Laboratory
22:
DIETZSCHOLD,
LITERATURE
Forces
The
animal of
C. C., AND H. HARKEMA. 1970. opossum.(Didelphis mrginiana)
erinary RUPPRECHT,
of Agriculture.
of Registry
D.C.,
1979.
infections
Department
Armed
International
search
Laboratory
Identification section.
Washington,
Journal
assis-
1982.
histologic
W.
animal.
Carolina.
177
183-189.
Pathology,
JURGELSKI,
MILLER,
North 22:
H. in
Carolina.
should interpret
technical
from
Science
GARDINER,
of the
parasites,
Thus, researchers infections as they
results. We thank part
a con-
as a labo(Sherwood
et al., that
didelphis,
above. of these
tance
and
virgmnmana)
thology,
1977).
The ratory al.,
parasite
pul-
of gastrointestinal parato opossum (Prestwood
et al.,
geiski,
Extensive
COMMUNICATIONS
1990.
21:
224-