Albrecht v. Graefes Arch. klin. exp. Ophthal. 211, 85-93 (1979)
Graefes Archiv fur klinische und experimentelle
Ophthalmologie 9 by Springer-Verlag 1979
Leukocyte Migration Inhibitory Factor in Primary and Recurrent Ocular Infections by Herpes simplex-Virus Preliminary Results Gfinther Grabner I * and Reinhart Jarisch 2 1 Department of Ophthalmology II, University of Vienna (Head: Prof. Dr. H. Slezak), AKH, Alserstr. 4, A-1090-Vienna, Austria and 2 Department of Dermatology I, University of Vienna (Head: Prof. Dr. J. Tappeiner), Vienna, Austria
Summary. Using a direct assay of leukocyte migration inhibitory factor (LMIF), cell-mediated immunity to herpes simplex virus (HSV) antigen was measured in patients who had primary and recurrent ocular HSV infections. Those with primary and recurrent epithelial disease had a significantly lower production of LMIF than a control population that had had no herpetic infection for at least a year or longer (P 20%. A valid statistical comparison, however, between the two groups is not possible, since patients were tested at different times after the onset of the disease and rapid changes (within a few days) in L M I F production were described (O'Reilly et al., 1977). 4. A healing of the dendritic ulcer without complications (in 3 patients) was not necessarily combined with a permanently elevated level of L M I F , if this factor was produced in detectable levels at all. To reach statistically valid results, a time series analysis with a greater number of patients seems necessary. Figure 2 shows the MI with HSV antigen in 19 patients with recurrent ocular HSV infections. The 9 patients with deep stromal involvement (disciform keratitis, iridocyclitis) had a mean MI of 22.56+ 16.96% (range: 0-45%). This value was n o t significantly different from the one for the control group. In contrast, 10 patients with recurrent dendritic ulcers only, without significant inflammatory signs in the corneal stroma or the anterior uvea, had a mean MI of 3.2+_4.71% (range: 0-14%). Statistical comparison between the
Leukocyte Migration Inhibitory Factor
Migration inhibition with HSVantigen
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Fig. 1. Patients with primary ocular HSV infection (n = 17); m e a n MI = 11,47 +_ 14,59%
Migration inhibition 50% with HSV antigen
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two groups showed this difference to be significant (P