International Journal of Pediatric Otorhinolaryngology 79 (2015) 1172
Contents lists available at ScienceDirect
International Journal of Pediatric Otorhinolaryngology journal homepage: www.elsevier.com/locate/ijporl
Letter to the Editor Letter to the Editor regarding ‘‘Protective effect of resveratrol against cisplatin-induced ototoxicity in HEI-OC1 auditory cells’’
Conflict of interest
Dear Sir,
Financial disclosure
We would like to ask you give an opportunity to point out some aspects of the study of Lee et al. [1]. Their valuable study searched the effects of resveratrol against cisplatin-induced ototoxicity in vitro and pointed out that resveratrol may be effective in protection against cisplatin induced ototoxicity by inhibiting ROS production in HEI-OC1 cell culture; saying that it was the first study according to their knowledge. However, our team has also worked on otoprotective effects of resveratrol against cisplatin ototoxicity on the same cell line before and published it already in 2013 [2]. By using MTT, TUNEL analysis and transmission electron microscopy of cells in our study, we formerly found out that resveratrol in low doses had an in vitro otoprotective effect against cisplatin induced ototoxicity. Moreover, apoptosis related gene expression analyses by real time PCR showed that this effect was mainly related with down regulation of apoptotic genes. The authors also rightfully noted that, although their in vitro study confirmed the otoprotective effect of resveratrol, it should not be interpreted as being clinically effective for cancer patients and animal studies were necessary. Our team has conducted an in vivo research after our first study and our clinical results implied that, protective effect of orally administrated resveratrol against cisplatin ototoxicity was questionable. As shown both audiologically and electron microscopically, high doses of resveratrol worsened the hearing much more. Histopathologic changes were found to be less prominent only with low doses, but these findings never reflected to and correlated with the audiological tests [3]. We are happy that their study is in accordance with our two previous studies in many aspects; we would also like to say that the value of resveratrol for cancer patients needs to be uncovered.
http://dx.doi.org/10.1016/j.ijporl.2015.05.003 0165-5876/ß 2015 Elsevier Ireland Ltd. All rights reserved.
None declared.
None. References [1] S.H. Lee, H.S. Kim, Y.S. An, J. Chang, J. Choi, J. Im, Protective effect of resveratrol against cisplatin-induced ototoxicity in HEI-OC1 auditory cells, Int. J. Pediatr. Otorhinolaryngol. 79 (2015) 58–66. [2] Y. Olgun, Z. Altun, S. Aktas, P. Ercetin, G. Kirkim, M. Kiray, et al., Molecular mechanisms of protective effect of resveratrol against cisplatinium induced ototoxicity, J. Int. Adv. Otol. 9 (2) (2013) 145–152. [3] Y. Olgun, G. Kırkım, E. Kolatan, M. Kıray, A. Bagriyanik, A. Olgun, et al., Friend or foe? Effect of oral resveratrol on cisplatin ototoxicity, Laryngoscope 124 (3) (2014) 760–766.
Yu¨ksel Olgun* Dokuz Eylu¨l University, School of Medicine, Department of Otorhinolaryngology, Izmir, Turkey Zekiye Altun Dokuz Eylu¨l University, Institute of Oncology, Department of Basic Oncology, Izmir, Turkey Enis Alpin Gu¨neri Dokuz Eylu¨l University, School of Medicine, Department of Otorhinolaryngology, Izmir, Turkey *Corresponding
author. Tel.: +90 506 7021434 E-mail addresses: [email protected], [email protected] (Y. Olgun). 17 February 2015 Available online 14 May 2015
Contents lists available at ScienceDirect
International Journal of Pediatric Otorhinolaryngology journal homepage: www.elsevier.com/locate/ijporl
Letter to the Editor Letter to the Editor regarding ‘‘Protective effect of resveratrol against cisplatin-induced ototoxicity in HEI-OC1 auditory cells’’
Conflict of interest
Dear Sir,
Financial disclosure
We would like to ask you give an opportunity to point out some aspects of the study of Lee et al. [1]. Their valuable study searched the effects of resveratrol against cisplatin-induced ototoxicity in vitro and pointed out that resveratrol may be effective in protection against cisplatin induced ototoxicity by inhibiting ROS production in HEI-OC1 cell culture; saying that it was the first study according to their knowledge. However, our team has also worked on otoprotective effects of resveratrol against cisplatin ototoxicity on the same cell line before and published it already in 2013 [2]. By using MTT, TUNEL analysis and transmission electron microscopy of cells in our study, we formerly found out that resveratrol in low doses had an in vitro otoprotective effect against cisplatin induced ototoxicity. Moreover, apoptosis related gene expression analyses by real time PCR showed that this effect was mainly related with down regulation of apoptotic genes. The authors also rightfully noted that, although their in vitro study confirmed the otoprotective effect of resveratrol, it should not be interpreted as being clinically effective for cancer patients and animal studies were necessary. Our team has conducted an in vivo research after our first study and our clinical results implied that, protective effect of orally administrated resveratrol against cisplatin ototoxicity was questionable. As shown both audiologically and electron microscopically, high doses of resveratrol worsened the hearing much more. Histopathologic changes were found to be less prominent only with low doses, but these findings never reflected to and correlated with the audiological tests [3]. We are happy that their study is in accordance with our two previous studies in many aspects; we would also like to say that the value of resveratrol for cancer patients needs to be uncovered.
http://dx.doi.org/10.1016/j.ijporl.2015.05.003 0165-5876/ß 2015 Elsevier Ireland Ltd. All rights reserved.
None declared.
None. References [1] S.H. Lee, H.S. Kim, Y.S. An, J. Chang, J. Choi, J. Im, Protective effect of resveratrol against cisplatin-induced ototoxicity in HEI-OC1 auditory cells, Int. J. Pediatr. Otorhinolaryngol. 79 (2015) 58–66. [2] Y. Olgun, Z. Altun, S. Aktas, P. Ercetin, G. Kirkim, M. Kiray, et al., Molecular mechanisms of protective effect of resveratrol against cisplatinium induced ototoxicity, J. Int. Adv. Otol. 9 (2) (2013) 145–152. [3] Y. Olgun, G. Kırkım, E. Kolatan, M. Kıray, A. Bagriyanik, A. Olgun, et al., Friend or foe? Effect of oral resveratrol on cisplatin ototoxicity, Laryngoscope 124 (3) (2014) 760–766.
Yu¨ksel Olgun* Dokuz Eylu¨l University, School of Medicine, Department of Otorhinolaryngology, Izmir, Turkey Zekiye Altun Dokuz Eylu¨l University, Institute of Oncology, Department of Basic Oncology, Izmir, Turkey Enis Alpin Gu¨neri Dokuz Eylu¨l University, School of Medicine, Department of Otorhinolaryngology, Izmir, Turkey *Corresponding
author. Tel.: +90 506 7021434 E-mail addresses: [email protected], [email protected] (Y. Olgun). 17 February 2015 Available online 14 May 2015
