Journal of Analytical Toxicology 2015;39:691 –697 doi:10.1093/jat/bkv100 Advance Access publication August 30, 2015
Article
LC –MS-MS Analysis of Urinary Biomarkers of Imazalil Following Experimental Exposures Moosa H. Faniband*, Margareta Littorin, Eva Ekman, Bo A.G. Jo¨nsson and Christian H. Lindh Division of Occupational and Environmental Medicine, Department of Laboratory Medicine, Faculty of Medicine, Lund University, SE-221 85 Lund, Sweden *Author to whom correspondence should be addressed. Email: [email protected]
Imazalil (IMZ) is a fungicide used in the cultivation of vegetables, such as cucumbers, in green houses or post-harvest on fruit to avoid spoilage due to fungal growth. Agricultural workers can be occupationally exposed to IMZ and the general public indirectly by the diet. The purpose of this study was to develop and validate an LC– MS-MS method for the analysis of IMZ in human urine. The method used electrospray ionization and selected reaction monitoring in the positive mode. Excellent linearity was observed in the range 0.5 – 100 ng/mL. The limit of detection of the method was 0.2 ng/mL, and the limit of quantitation 0.8 ng/mL. The method showed good within-run, between-run and between-batch precision, with a coefficient of variation
Article
LC –MS-MS Analysis of Urinary Biomarkers of Imazalil Following Experimental Exposures Moosa H. Faniband*, Margareta Littorin, Eva Ekman, Bo A.G. Jo¨nsson and Christian H. Lindh Division of Occupational and Environmental Medicine, Department of Laboratory Medicine, Faculty of Medicine, Lund University, SE-221 85 Lund, Sweden *Author to whom correspondence should be addressed. Email: [email protected]
Imazalil (IMZ) is a fungicide used in the cultivation of vegetables, such as cucumbers, in green houses or post-harvest on fruit to avoid spoilage due to fungal growth. Agricultural workers can be occupationally exposed to IMZ and the general public indirectly by the diet. The purpose of this study was to develop and validate an LC– MS-MS method for the analysis of IMZ in human urine. The method used electrospray ionization and selected reaction monitoring in the positive mode. Excellent linearity was observed in the range 0.5 – 100 ng/mL. The limit of detection of the method was 0.2 ng/mL, and the limit of quantitation 0.8 ng/mL. The method showed good within-run, between-run and between-batch precision, with a coefficient of variation
