AIDS RESEARCH AND HUMAN RETROVIRUSES Volume 8, Number 8, 1992 Mary Ann Liebert, Inc., Publishers
Isotype of Anti-SIV Responses in Infected Rhesus Macaques in Animals Immunized by Mucosal Routes
and
Downloaded by Western Sydney University from www.liebertpub.com at 01/13/19. For personal use only.
JERRY R. McGHEE,' MASAFUMI YAMAMOTO,1 DONG WON KANG,1 JOHN H. ELDRIDGE,1 JIRI MESTECKY,1 ZINA MOLDOVEANU,1 RICHARD COMPANS,1 HIROSHI KIYONO,1 CHRISTOPHER MILLER,2 MARTA MARTHAS,2 AGEGNEHU GETTIE,2 and PRESTON A. MARX3
WE
have assessed the
isotype and level of SIV-specific
responses in sera and vaginal washes (VW) of rhesus macaques infected with SIVmac by either genital or systemic routes. In both groups, serum lgG anti-SIV antibodies appeared by 30 days after infection and continued to increase throughout
infection. Although elevations in total IgM and IgA were seen in infected animals, specific antibodies of these two isotypes were low. In terminally ill animals, both serum and VW contained IgG anti-SIV antibodies, suggesting that the VW responses were serum derived. These results demonstrated that both mucosal and systemic routes of SIVmac infection lead to an identical isotype pattern of anti-SIVma(;-specific responses. In separate studies, two groups of macaques were immunized with SIVmac in microspheres by intramuscular (i.m.) (8 animals) or oral (4 animals) routes (see Marx et al. Abstract). Primary i.m.
SIVmac
University of Alabama at Birmingham, UAB Station. Birmingham, 2CRPRC, Pedrick and Hutchison Roads, Davis, CA 95616. 'NMRPRL. Holiman Air Force Base, Building 1264, NM 88330.
immunization induced serum IgG anti-SIV responses, which enhanced by second and especially by the third immunization. Orally immunized macaques had essentially no serum antibodies, but some IgA anti-SIV responses were seen in gut wash and VW, especially after the third oral administration. Current studies are evaluating serum and mucosal antibody responses of i.m.-primed animals following either intratracheal or oral immunization. (This work was supported by U.S. PHS Grants Al 28147 and Al 30366). were
AL 35294.
1389
reprint requests to: Jerry R. McGhee University of Alabama
Address
UAB Station AL 35294
Birmingham,
Isotype of Anti-SIV Responses in Infected Rhesus Macaques in Animals Immunized by Mucosal Routes
and
Downloaded by Western Sydney University from www.liebertpub.com at 01/13/19. For personal use only.
JERRY R. McGHEE,' MASAFUMI YAMAMOTO,1 DONG WON KANG,1 JOHN H. ELDRIDGE,1 JIRI MESTECKY,1 ZINA MOLDOVEANU,1 RICHARD COMPANS,1 HIROSHI KIYONO,1 CHRISTOPHER MILLER,2 MARTA MARTHAS,2 AGEGNEHU GETTIE,2 and PRESTON A. MARX3
WE
have assessed the
isotype and level of SIV-specific
responses in sera and vaginal washes (VW) of rhesus macaques infected with SIVmac by either genital or systemic routes. In both groups, serum lgG anti-SIV antibodies appeared by 30 days after infection and continued to increase throughout
infection. Although elevations in total IgM and IgA were seen in infected animals, specific antibodies of these two isotypes were low. In terminally ill animals, both serum and VW contained IgG anti-SIV antibodies, suggesting that the VW responses were serum derived. These results demonstrated that both mucosal and systemic routes of SIVmac infection lead to an identical isotype pattern of anti-SIVma(;-specific responses. In separate studies, two groups of macaques were immunized with SIVmac in microspheres by intramuscular (i.m.) (8 animals) or oral (4 animals) routes (see Marx et al. Abstract). Primary i.m.
SIVmac
University of Alabama at Birmingham, UAB Station. Birmingham, 2CRPRC, Pedrick and Hutchison Roads, Davis, CA 95616. 'NMRPRL. Holiman Air Force Base, Building 1264, NM 88330.
immunization induced serum IgG anti-SIV responses, which enhanced by second and especially by the third immunization. Orally immunized macaques had essentially no serum antibodies, but some IgA anti-SIV responses were seen in gut wash and VW, especially after the third oral administration. Current studies are evaluating serum and mucosal antibody responses of i.m.-primed animals following either intratracheal or oral immunization. (This work was supported by U.S. PHS Grants Al 28147 and Al 30366). were
AL 35294.
1389
reprint requests to: Jerry R. McGhee University of Alabama
Address
UAB Station AL 35294
Birmingham,
