Isolation of Nascent Transcripts with Click Chemistry.

Steady-state levels of cellular RNA are determined by both transcriptional rate and RNA half-life. Commonly used methods for transcriptional analysis ...
283KB Sizes 4 Downloads 11 Views

Recommend Documents


Liposome functionalization with copper-free "click chemistry".
The modification of liposomal surfaces is of interest for many different applications and a variety of chemistries are available that makes this possible. A major disadvantage of commonly used coupling chemistries (e.g. maleimide-thiol coupling) is t

Nascent RNA transcripts facilitate the formation of G-quadruplexes.
Recent discovery of the RNA/DNA hybrid G-quadruplexes (HQs) and their potential wide-spread occurrence in human genome during transcription have suggested a new and generic transcriptional control mechanism. The G-rich sequence in which HQ may form c

Super-Resolution Imaging of Plasma Membrane Proteins with Click Chemistry.
Besides its function as a passive cell wall, the plasma membrane (PM) serves as a platform for different physiological processes such as signal transduction and cell adhesion, determining the ability of cells to communicate with the exterior, and for

Expedient Synthesis of SMAMPs via Click Chemistry.
A novel series of synthetic mimics of antimicrobial peptides (SMAMPs) containing triazole linkers were assembled using click chemistry. While only moderately active in buffer alone, an increase in antimicrobial activity against Staphylococcus aureus

Surface functionalization of exosomes using click chemistry.
A method for conjugation of ligands to the surface of exosomes was developed using click chemistry. Copper-catalyzed azide alkyne cycloaddition (click chemistry) is ideal for biocojugation of small molecules and macromolecules to the surface of exoso

Dual-functionalized nanostructured biointerfaces by click chemistry.
The presentation of biologically active molecules at interfaces has made it possible to investigate the responses of cells to individual molecules in their matrix at a given density and spacing. However, more sophisticated methods are needed to creat

A click chemistry approach to secosteroidal macrocycles.
A new synthetic pathway towards secosteroidal macrocycles was described via a reaction of cycloaddition as the key step. The characteristic (1)H and (13)C NMR spectroscopic features of the synthesized compounds are reported.

In vivo targeting through click chemistry.
Targeting small molecules to diseased tissues as therapy or diagnosis is a significant challenge in drug delivery. Drug-eluting devices implanted during invasive surgery allow the controlled presentation of drugs at the disease site, but cannot be mo

Photo-Triggered Click Chemistry for Biological Applications.
In the last decade and a half, numerous bioorthogonal reactions have been developed with a goal to study biological processes in their native environment, i.e., in living cells and animals. Among them, the photo-triggered reactions offer several uniq

Click Chemistry-Mediated Nanosensors for Biochemical Assays.
Click chemistry combined with functional nanoparticles have drawn increasing attention in biochemical assays because they are promising in developing biosensors with effective signal transformation/amplification and straightforward signal readout for