J. Biochem., 80, 1001-1009 (1976)

Isolation and Characterization of Nerve Growth Factor from the Venom of Naja naja atra1 Shoei FURUKAWA and Kyozo HAYASHI Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto, Kyoto 606 Received for publication, May 4, 1976

Nerve growth factor was isolated from the venom of Naja naja atra by ion exchange and gel permeation chromatography and was found to be homogeneous by disc gel electrophoresis. The molecular weight was estimated to be approximately 20,000 by gel filtration and 22,000 by ultracentrifugation. This protein, which showed an isoelectric point of pH 7.02, probably consists of two subunits of equal molecular weight which are held together or interact with each other noncovalently. The biological activity survives treatment by a number of proteolytic enzymes, such as trypsin [EC 3.4.21.4], chymotrypsin [EC 3.4.21.1], and pepsin [EC 3.4.23.1].

Nerve growth factor (NGF) is a protein which promotes the growth of the sympathetic chain ganglia in vivo and a halo-like outgrowth of nerve fibers in vitro. This factor was originally discovered in certain mouse sarcomas

Isolation and characterization of nerve growth factor from the venom of Naja naja atra.

Nerve growth factor was isolated from the venom of Naja naja atra by ion exchange and gel permeation chromatography and was found to be homogeneous by...
1MB Sizes 0 Downloads 0 Views